Özlem ÇAKICI, Gürsel ERGEN

Light and scanning electron microscope investigations on the structure of the proventriculus in Melanogryllus desertus (Pallas) (Orthoptera: Gryllidae)

Bu çalışma 2005-2008 yılları arasında Ege Üniversitesi Kampüsü bünyesindeki Omurgasız Kültür ve Araştırma Laboratuvarında gerçekleştirildi. Bu çalışmada, Melanogryllus desertusta proventrikulus morfolojisini ışık ve taramalı elektron mikroskobuyla incelemeyi amaçladık. Genel proventrikulus morfolojisi sklerotik apendislerin altı internal sırada düzenlenmesiyle diğer Gryllidae türlerine benzerdir. Yapısal olarak, sklerotik apendisler yuvarlak dişçiklerle donanan tek orta diş ve iki tane lateral dişten oluşmuştur. Genelde dört orta dişçik bulunmasına rağmen sayısı beş ya da altı olabilir. Ayrıca sklerotik apendisler arasında sklerotik loblar ve sklerotik uzantılar mevcuttur ve yoğun kitin kıllıdır.

Melanogryllus desertus (Pallas) (Orthoptera: Gryllidae) ta proventrikulusun yapısı üzerine ışık ve taramalı elektron mikroskobu araştırmaları

This study was carried out between the years 2005 and 2008 in the Invertebrate Culture and Research Laboratory at Ege University, Turkey. In this work, we aimed to investigate the proventriculus morphology of Melanogryllus desertus using light and scanning electron microscopy. The general morphology of the proventriculus resembles other Gryllidae species in that sclerotized appendices are arranged in six internal rows. Structurally, sclerotized appendices are composed of a middle tooth and two lateral teeth, both adorned with denticles rounded in shape. Although four middle denticles are found in general, their numbers vary to as many as five or six. In addition, there are sclerotized lobes and sclerotized partitions among sclerotized appendices, and they are densely setose.

PDF

___

Bailey, L., 1952. The action of the proventriculus of the worker honeybee, Apis mellifera L. Journal of Experimental Biology, 29: 310-317.
Bland, R. G. & D. C. F. Rentz, 1991. Studies in australian Gryllacrididae: the proventriculus as a taxonomic character. Invertebrate Taxonomy, 5: 443-455.
Caetano, F. H., 1988. Anatomia, histologia e histoquimica do sistema digestivo e excretor de opererias de formigas (Hymenoptera, Formicidae). Naturalia, 13: 129-174.
Fontanetti, C. S. & E. Zefa, 2000. Morphological characterization of the proventriculus of Gryllus assimilis Fabricius (Orthoptera, Gryllidae). Revista Brasileira de Zoology 17 (1): 193-198.
Fontanetti, C. S., E. Zefa, F. Passetti & A. Mesa, 2002. Morphological characterization and comparative analysis of the proventriculus from three species of Endecous Saussure, 1878 (Orthoptera: Gryllidae: Phalangopsinae). Entomotropica 17 (1): 15-23.
Judd, W. W., 1948. A comparative study of the proventriculus of orthopteroid insects with reference to its use in taxanomy. The Canadian Journal of Research 26 (D): 93-161.
Humason, G. L., 1979. Animal tissue techniques, 4 th edn. WH Freeman, San Francisco
Lebrun, D., 1985, Structures digestives et régimes alimentaires des Termites. Actes des Colloques Insectes Sociaux 2: 43-44.
Lebrun, D. & A. Lequet, 1985. Relations entre le régimes alimentaires et la structure du gésier des termites. Bulletin Societe Science Nature Quest France 7 (3): 126-139.
Li, Z.Y., B. Ren, Y. Zou, J. Zhang, & Y. Wang, 2011. The study of proventricular micromorphological characterization of ten Grylloidea species (Orthoptera: Grylloidea) from China. Zootaxa 2906: 52-60.
Lodos, N., 1975. Türkiye Entomolojisi genel, uygulamalı, faunistik (Ders Kitabı) Ege Üniversitesi Ziraat Fakültesi Yayınları No: 282 Bornova-İzmir.
Mesa, A., P. G. Novo, T. Rhoat, & C. Portugal, 2004. Morphological characters and karyology of Miogryllus piracicabensis Piza (Orthoptera: Gryllidae). Neotropical Entomology 33 (3): 337-340.
Nation, J. L., 1983. A new method using hexamethyldisilazane for preparation of soft insect tissues for scanning electron microscopy. Staining Technology 58: 347-351.
Nation, J. L., 2002. Insect Physiology and Biochemistry, CRC Press, Boca Raton, FL, 485p.
Peng, Y. S. & J. M. Marston, 1986. Filtering mechanism of the honey bee proventriculus. Physiological Entomology 11: 433-439.
Roche, R. K. & D. E. Wheeler, 1997. Morphological specializations of the digestive tract of Zacryptocerus rohweri (Hymenoptera: Formicidae). Journal of Morphology 234: 253-262.
Serrao, J. E., 2001. A comparative study of the proventricular structure in corbiculate apine (Hymenoptera, Apidae). Micron 32: 379-385.
Serrao, J. E., 2005. Proventriculus structure in solitary bees (Hymenoptera: Apoidea). Organism Diversity Evolution 5: 125-133.
Serrao, J. E., 2007. Proventricular structure in the bee tribe Augochlorini (Hymenoptera: Halictidae) Organism Diversity Evolution 7: 175-180.
Snoddgrass, R. E., 1935. Principles of Insect Morphology, Mc Graw Hill Book Co., Inc, New York, 667 pp.
Strobel, S., H. R. P Miller & A. Ferguson, 1981. Human intestinal mucosal mast cells: evaluation of fixation and staining techniques. Journal of Clinical Pathology 34: 851-858.
Szinwelski, N., M. S. Rodrigues, M. R. Pereira, J. E. Serrao, & C. F. Sperber, 2009. Proventriculus of three nemobiinae crickets (Ortoptera: Grylloidea: Trigonidiidae). Journal of Orthoptera Research 18 (1): 59-63.
Woodring, J. & M. W. Lorenz, 2007. Feeding, nutrient flow and functional gut morphology in the cricket Gryllus bimaculatus. Journal of Morphology 268: 815-825.