Zübeyir DEVRAN, İbrahim MISTANOĞLU, Tevfik ÖZALP

Farklı sayıda gerçek yapraklı döneme sahip domates fidelerinin Meloidogyne incognita (Kofoid & White, 1919) Chitwood, 1949’ya tepkileri

Domates dünyada yetiştiriciliği yapılan en önemli sebzelerden birisidir. Kök-ur nematodları domateste önemli düzeyde ekonomik kayıplara neden olmaktadır. Kök-ur nematodlarına dayanıklı domates çeşitlerinin geliştirilmesi en önemli mücadele yöntemidir. Domateste kök-ur nematodlarına dayanıklılık biyolojik testler ve moleküler markörler tarafından belirlenebilmektedir. Moleküler markörler ıslah programlarında dayanıklılık geninin tespitinde yaygın şekilde kullanılmaktadır. Bununla birlikte dayanıklılık geninin performansının belirlenmesi için biyolojik testler gereklidir. Nematod virülensliği, nematod sayısı, toprak sıcaklığı ve fidenin dönemi gibi farklı parametreler biyolojik testin performansını etkilemektedir. Bu çalışmada, farklı sayıda gerçek yaprak dönemine sahip domates fidelerinin M. incognita’ nın S6 izolatına tepkisi kontrollü koşullar altında karşılaştırılmıştır. Fideler M. incognita inokulasyonuna farklı farklı tepkiler göstermiştir. Sonuçlar, biyolojik test için domates fidesinin döneminin önemli olduğunu ve nematod testi için dördüncü gerçek yapraklı döneme sahip fidelerin en uygun olduğunu göstermiştir. Bu sonuçlar, domates ıslahında kullanılacak olan kök-ur nematodlar testlerinin optimizasyonuna yardım edecektir.

Anahtar Kelimeler:

Biyolojik test, Meloidogyne, domates fidesi, reaksiyon

Response of tomato seedlings with different number of true leavesto Meloidogyne incognita (Kofoid & White, 1919) Chitwood, 1949

Tomato is one of the most important vegetables grown throughout the world. Root-knot nematodes cause significant economic yield losses in tomato. Development of tomato cultivars which are resistant to root-knot nematodes is the most effective management method. In tomato, resistance to root-knot nematodes is determined by bioassay and molecular markers. Molecular markers are commonly used to screen for resistance genes in breeding programs. However, bioassays are also required for identification of the performance of resistance genes. Different parameters such as stage of seedling, soil temperature, nematode quantity and nematode virulence effect performance of bioassays. In the present study, the response of tomato seedlings with different numbers of true leaves to Meloidogyne incognita isolate S6 was compared under controlled conditions. Seedlings showed different reactions to M. incognita inoculation. The results indicated that stage of tomato seedlings can be important for bioassay and that tomato seedlings with four true leaves are best for nematode testing. These results will help in the optimization of root-knot nematode tests used in tomato breeding.

Keywords:

Bioassay, Meloidogyne, tomato seedling, reaction,

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