Quantitative estimation of residues of neem compounds (NfC and NC) and dimilin (IGR) in treeted Callosobruchus analis F. and contact media (Filter paper and glass) by HPLC

Employing high performance liquid chromatography, quantitative estimation of neem compounds, NfC and NC, was carried out in treated insects, Cailosobruchus analis, and in contact media, in comparison with an IGR, Diflubenzuron (Dimilin). Adult specimens of Cailosobruchus analis were treated with impregnated filter paper mimicking an absorbent surface and treated glass mimicking a non-absorbent surface of grain storing containers. In the case of filter paper impregnation,.the recovery of NfC was 4.57 ug/10 ul and 1.28 ug/10 pi in treated insect samples, and 0.71 ug/10 ul and 0.50 ug/10 ul in treated filter paper samples after 24 and 144 h of treatment, respectively. Recovery of NC was 0.71 ug/10 ul after 24 h and no trace was found after 144 h in treated insect samples, whereas treated filter paper samples showed 0.21 ug/10 ul and 0.07 ug/10 ul recovery after 24 and 144 h of treatment, respectively. The recovery of Dimilin was 0.14 ug/10 ul and 0.08 ug/10 ul in treated insect samples, and 0.36 ug/10 ul and 0.14 ug/10 ul in treated filter paper samples after 24 and 144 h of treatment, respectively. In the case of the glass film method, NC treated insects showed 1.42 ug/10 ul and 0.71 ug/10 ul recovery, while the glass rinsed samples showed 0.71 ug/10 ul and 0.07 ug/10 ul recovery after 24 and 144 h of treatment, respectively. Dimilin treated C. analis showed 0.84 ug/10 ul and 0.33 ug/10 ul recovery after 24 and 144 h of treatment, while the glass rinsed samples showed 0.12 ug/10 ul recovery after 24 h and no trace was found after 144 h of treatment. NfC extract showed no significant effect by this method and therefore samples were not analysed.

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