Morphological and molecular characterization of Myxobolus puntiusii n. sp. (Cnidaria: Myxosporea) infecting Puntius sophore Hamilton, 1822 from Ranjit Sagar Wetland, Punjab (India)

The pool barb,Puntius sophoreHamilton, 1822, is a tropical freshwater cyprinid fish native to inland waters in Asia. Herein,a novel myxozoan from plasmodia on the caudal fin ofP. sophorefrom Ranjit Sagar Wetland in Punjab, India is described. Myxosporeswere consistent with the genusMyxobolus , ellipsoidal in frontal view, lemon-shaped in lateral view; length 7.76 ± 0.28 μm, width 5.36± 0.15 μm. There were two polar capsules of unequal size: a larger polar capsule, length 3.01 ± 0.08 μm, width 1.83 ± 0.11 μm, with 6 7turns of the polar filament, and a smaller polar capsule, length 1.71 ± 0.15 μm, width 0.94 ± 0.07 μm, with 3 4 turns of the polar filament.The 1183 bp 18S rDNA sequence was up to 95% similar toMyxobolusspp. from other cyprinid fishes in India. Given the novel host,unique myxospore morphology, and 18S rDNA sequence, we proposeMyxobolus puntiusiin. sp.

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Akhmerov AK (1954). On closely related species of a new myxosporean genus. Dokl Akad Nauk SSS 97: 1101-1103.
Allamuratov B (1966). New species of parasitic protozoa found in the fish of South-Surkham Reservoir. Uzb Biol Zh 10: 58-60.
Altschul SF, Gish W, Miller W, Myers EW, Lipman DJ (1990). Basic local alignment search tool. J Mol Biol 215: 403-410.
Atkinson SD, Bartoová-Sojková P, Whipps CM, Bartholomew JL (2015). Approaches for characterizing myxozoan species. In: Okamura B, Gruhl A, Bartholomew JL, editors. Myxozoan Evolution, Ecology and Development. Cham, Switzerland: Springer International Publishing, pp. 111-124.
Attri R (2015). Taxonomic evaluation of myxozoan parasites infecting fresh water fishes in Punjab. PhD, Punabi University, Patiala, Punjab, India.
Bartholomew JL, Whipple MJ, Stevens DG, Fryer JL (1997). The life cycle of Ceratomyxa shasta , a myxosporean parasite of salmonids, requires a freshwater polychaete as an alternate host. J Parasitol 83: 859-868.
Basu S, Haldar DP (2003). Observations on two new thelohanelloid species (Myxozoa: Bivalvulida) from Indian major carps of West Bengal, India. J Parasitol App Anim Biol 12: 15-24.
Chen CL, Ma CL (1998). Fauna Sinica. Myxozoa, Myxosporea. Beijing, China: Science Press Beijing.
Gupta A, Kaur H (2016). Morphological, histopathological and molecular characterization of Thelohanellus theinensis n. sp. (Cnidaria: Myxosporea) infecting an Indian major carp, Labeo bata in a cold water wetland in Punjab (India). J Parasit Dis: 1-10. doi:10.1007/s12639-016-0856-4.
Hall T (2011). BioEdit: An important software for molecular biology. GERF Bulletin of Biosciences 2: 60-61.
Kaur H, Gupta A (2015). Genetic relatedness provides support for a species complex of myxosporeans infecting the Indian major carp, Labeo rohita . Anim Biol 65: 337-347.
Kaur H, Gupta A (2017). Molecular and phylogenetic characterization of Thelohanellus boggoti Qadri, 1962 (Cnidaria, Myxosporea, Bivalvulida) infecting the fin of Indian minor carp Labeo dero (Hamilton, 1822). Mol Biol Res Comm 6: 13-21.
Kaur H, Katoch A, Gupta M (2014a). Thelohanellus filli sp. n., a pathogenic myxosporean infecting gills of cultured carp, Labeo rohita (Hamilton 1822) in Punjab, India. Species 10: 31-38.
Kaur H, Singh R (2009). A new myxosporean species, Myxobolus eirasi sp. nov., a known species M. venkateshi Seenappa and Manohar (1981) from the India major carp fish Cirrhina mrigala (Ham). Protistol 6: 126-130.
Kaur H, Singh R (2011d). Two new species of Myxobolus (Myxozoa: Myxosporea: Bivalvulida) infecting Indian freshwater fishes in Punjab wetlands (India). Parasitol Res 108: 1075-1082.
Kaur H, Singh R (2012a). A synopsis of the species of Myxobolus Bütschli, 1882 (Myxozoa: Bivalvulida) parasitizing Indian fishes and a revised dichotomous key to myxosporean genera. Syst Parasitol 81: 17-37.
Kaur H, Singh R, Katoch A, Attri R, Dar SA, Gupta A (2016). Species diversity of the genus Thelohanellus Kudo , 1933 (Myxozoa: Bivalvulida) parasitizing fishes in Indian subcontinent. J Parasit Dis: 1-8. doi:10.1007/s12639-016-0836-8.
Kent ML, Andree KB, Bartholomew JL, El-Matbouli M, Desser SS, Devlin RH, Feist SW, Hedrick RP, Hoffman RW, Khattra J et al. (2001). Recent advances in our knowledge of the Myxozoa. J Eukaryot Microbiol 48: 395-413.
Kimura M (1980). A simple method for estimating evolutionary rate of base substitution through comparative studies of nucleotide sequences. J Mol Evol 16: 111-120.
Landsberg JH, Lom J (1991). Taxonomy of the genera of the Myxobolus / Myxosoma group (Myxobolidae: Myxosporea), current listing of species and revision of synonyms. Sys Parasitol 18: 155-186.
Liu XH, Batueva MD, Zhao YL, Zhang JY, Zhang QQ, Li TT, Li AH (2016). Morphological and molecular characterisation of Myxobolus pronini n. sp. (Myxozoa: Myxobolidae) from the abdominal cavity and visceral serous membranes of the gibel carp Carassius auratus gibelio (Bloch) in Russia and China. Parasit Vectors 9: 562. doi: 10.1186/s13071-016-1836-3.
Lom J, Dykovà I (1992). Myxosporidia (Phylum Myxozoa). In: Protozoan Parasites of Fishes. Developments in Aquaculture and Fisheries. Lom J and Dykovà I, editors. Amsterdam, the Netherlands: Elsevier, pp. 159-235.
Pote LM, Hanson LA, Shivaji R (2000). Small subunit ribosomal RNA sequences link the cause of proliferative gill disease in channel catfish to Henneguya n. sp. (Myxozoa: Myxosporea). J Aquat Anim Health 12: 230-240.
Ronquist F, Huelsenbeck JP (2003). Bayesian phylogenetic inference under mixed models. Biogeosciences 19: 1572-1574.
Sakiti N, Blanc E, Marques A, Bouix G (1991). Myxosporidies (Myxozoa: Myxosporea) du genre Myxobolus Bütschli, 1882 parasites de poisons cichlidae du lae Nokoue au Benimm (Afrique de l Ouest). J Afr Zool 105: 173-186.
Shulman SS (1966). Myxosporidia of the USSR . Moscow and Leningrad, Russia: Nauka.
Shulman SS (1984). Parasitic protozoa. In: Key to Parasites of Freshwater Fishes of the USSR. Vol. I. Bauer ON, editor. Leningrad, Russia: Nauka.
Singh R, Kaur H (2015). Two new and one already known species of the genus Thelohanellus (Myxozoa: Myxosporea: Bivalvulida) parasitizing fresh water fishes in wetlands of Punjab, India. Biologia 70: 85-93.
Székely C, Cech G, Chaudhary A, Borzak R, Hridaya S S, Molnár K (2014). Myxozoan infections of the three Indian major carps in fish ponds around Meerut, UP, India, with descriptions of three new species, Myxobolus basuhaldari sp. n., M. kalavatiae sp. n. and M. meerutensis sp. n., and the redescription of M. catlae and M. bhadrensis. Parasitol Res 114: 1301-1311.
Tamura K, Peterson D, Peterson N, Stecher G, Nei M Kumar S (2013). MEGA6: Molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance and maximum parsimony methods. Mol Biol Evol 28: 2731-2739.
Yakovchuk TA (1979). A new species of the genus Myxobolus (Myxosporidia: Myxobolidae) from the gill filaments of the carp. Parasitol 13: 635-636.
Yokoyama H, Liyanage YS, Sugai A, Wakabayashi H (1998). Hemorrhagic thelohanellosis of color carp caused by Thelohanellus hovorkai (Myxozoa: Myxosporea). Fish Pathol 33: 85-89.
Zhao Y, Sun C, Kent ML, Deng J, Whipps CM (2008). Description of a new species of Myxobolus (Myxozoa: Myxobolidae) based on morphological and molecular data. J Parasitol 94: 737-742.