Nuri YİĞİT, Tolga KANKILIÇ, Reyhan ÇOLAK, Ercüment ÇOLAK

Mesocricetus brandti Nehring, 1898 ve Mesocricetus auratus (Waterhouse, 1839) (Mammalia: Rodentia)’da Allozim Varyasyonlar› ve Genetik Farkl›l›klar

Anadolu ve ‹ran’da yay›l›fl gösteren Mesocricetus brandti ve Mesocricetus auratus türlerinin 5 populasyonunun allozim varyasyonu 20 gen lokusunun elektroforetik analiziyle incelenmifltir. Analiz edilen 20 gen lokusundan 11’i monomorfiktir ve bütün populasyonlarda ayn› allelde fikse olmufltur. 9 lokus polimorfiktir ve tek bir lokus M. auratus ve M. brandti populasyonlar› aras›nda de¤iflkenlik göstermifltir. Bütün populasyonlar için ortalama polimorfik lokus yüzdesi 24,7 ve 5,9 ile 41,2 aras›nda de¤iflmektedir. Fiksasyon indeksinin ortalama de¤eri FST = 0,0748 olup M. brandti populasyonlar›nda % 7 genetik farkl›l›k göstermektedir. Bu FST de¤eri orta derece genetik farkl›l›¤› göstermektedir. FST’den hesaplanan göç say›s› (Nm = 3,09) de¤eri etkili gen ak›fl›n›n oldu¤unu önermektedir. Bütün populasyonlar için (M. brandti ve M. auratus) ortalama heterozigotluk (gözlenen= Ho) 0,069’d›r ve 0,029 ile 0,118 aras›nda de¤iflmektedir. M. brandtii ve M. auratus’un ortalama Ho de¤erleri s›ras›yla Ho = 0,080 ve Ho = 0,029 olarak saptanm›flt›r. M. brandti populasyonlar› aras›nda Nei’nin genetik mesafesi D = 0,006 den D = 0,026’ya kadar de¤iflmektedir. Nei’nin genetik mesafesi iki tür aras›nada D = 0,102’den 0,122’e kadar de¤iflkenlik göstermektedir.

Anahtar Kelimeler:

Mesocricetus spp, allozim, varyasyon, Türkiye

Allozyme Variations and Genetic Differentiation in Mesocricetus brandti Nehring, 1898 and Mesocricetus auratus (Waterhouse, 1839) (Mammalia: Rodentia)

Allozyme variations were investigated by the electrophoretic analysis of 20 gene loci in 5 populations of Mesocricetus brandti and Mesocricetus auratus from Anatolia and Iran. Of the 20 loci analysed, 11 were monomorphic and fixed for the same allele in all 5 populations, 9 loci were polymorphic, and 1 locus differed between M. auratus and M. brandti populations. The overall mean of polymorphic loci for all the populations was 24.7% (range: 5.9%-41.2%). The mean fixation index value was FST = 0.0748, indicating a 7% genetic variation in the M. brandti populations. The obtained FST values indicated that there are moderate genetic differences between the populations of M. brandti. The finding that the number of migrants (Nm) was 3.09 also suggests effective gene flow across populations. The overall mean heterozygosity (Ho = direct count) for all populations (M. brandti and M. auratus) was 0.069 (range: 0.029-0.118 at different locations). The mean heterozygosity of M. brandti and M. auratus was Ho = 0.080 and Ho = 0,029, respectively. Nei's measure of genetic distance varied from D = 0.006 to D = 0.026 between populations of M. brandti. Nei's distances varied from D = 0.102 to D = 0.122 between M. auratus and M. brandti populations.

Keywords:

Mesocricetus spp., allozyme, variation, Turkey,

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