Ramazan URANLI, Osman PARLAK, Ebru GÖNCÜ

Investigation of the inluence of the juvenile hormone analogue fenoxycarb on major hemolymph proteins of the silkworm Bombyx mori during the last larval instar

Fenoksikarb 0-ethyl N-(2-(4-pheoxyphenoxy)-ethyl) karbamat, ipekböceği Bombyx mori’yi de içeren farklı böcek türlerinde en etkili juvenil hormon analoğudur. Bu çalışmada, fenoksikarb kullanılarak ipekböceği Bombyx mori’d e beşinci larval evre süresince temel hemolenf proteinleri üzerine juvenil hormonun etkisi ve bu proteinlerle juvenil hormon arasındaki bağlantının aydınlatılması amaçlanmıştır. Bombyx mori’de 5. larval evrede topikal fenoksikarb uygulamaları 4. larval deri değişiminden hemen sonra (0.gün) ve pupa öncesi periyodun başlangıcında (6.gün) gerçekleştirilmiştir. 0 günde fenoksikarb uygulaması her iki eşeyde de 5. larval evreyi uzatmış ve total hemolenf protein içeriğinin artışını uyarmıştır. Altıncı gün fenoksikarb uygulamaları sonrasında larval evrenin süresinde herhangi bir farklılık olmamıştır. Elektroforetik olarak belirlenen temel hemolenf proteinleri şu şekilde gruplandırılmıştır: 30K proteinleri, depo proteinleri (72 kDa, 76 kDa), vitellogenin (178 kDa, 42 kDa), lipoforin (230 kDa). Çalışmamızın sonucunda temel hemolenf proteinlerinin fenoksikarb’a hassasiyetlerinin farklı olduğu; uygulama gününün ve eşey faktörünün önemli olduğu sonucuna varılmıştır

İpekböceği Bombyx mori’de, son larval evre süresince juvenil hormon analoğu fenoksikarb’ın temel hemolenf proteinleri üzerine etkisinin araştırılması

Fenoxycarb 0-ethyl N-(2-(4-pheoxyphenoxy)-ethyl) carbamate is the most potent juvenile hormone analogue (JHA) against a variety of insect species including the silkworm Bombyx mori. In this study, we aimed to demonstrate the efects of this juvenile hormone on major hemolymph proteins of Bombyx mori during the ith larval instar, by using fenoxycarb and clarifying the interactions between these proteins and the juvenile hormone. Topical applications of fenoxycarb to the ith instar larvae of Bombyx mori were performed immediately ater the fourth ecdysis (on day 0) and at the beginning of the prepupal period (on day 6). Fenoxycarb application on day 0 increased the length of instar in both sexes and induced high total protein content in the hemolymph. When fenoxycarb application was performed on day 6, there was no diference in the length of larval instar and a similar total protein amount was reached with control. Detected hemolymph proteins are grouped as follows by using sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS- PAGE): storage proteins (72 kDa-76 kDa), 30kDa proteins, lipophorin (230 kDa), and vitellogenins (178 kDa and 42 kDa). Our results suggest that major hemolymph proteins have diferent sensitivities to fenoxycarb and that application days and sex are more important factors to get response.

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