Nikhat YASMIN, M. Farhanullah KHAN, Muhammad Samiullah CHANNA, Zeeshan ALI

Effects of a Neem Sample on Protein Patterns of Bactrocera cucurbitae

Bactrocera cucurbitae (Coquillett) adults were reared with 5% honey solution, sugar, and protein-hydrolysate. Females were provided to lay eggs on larval rearing media in a bottle gourd. Twenty third-instar larvae of the same size and age were exposed to filter papers treated with varying doses of a neem sample in 90 mm diameter petri dishes for 24 h. A probit-mortality curve was drawn to determine the LC50 of the sample. LC50 dose of the sample was calculated as 5.6%. Thin layer chromatography was used to determine the effects of neem sample on the protein patterns of the treated insect. The Rf-values of separated proteins (peptides) were determined and compared to those of the untreated control.

Anahtar Kelimeler:

Bactrocera cucurbitae, insect rearing, protein patterns, Azadirachta indica

Effects of a Neem Sample on Protein Patterns of Bactrocera cucurbitae

Keywords:

Bactrocera cucurbitae, insect rearing, protein patterns, Azadirachta indica,

PDF

___

Agarwal, M.L., Sarma, D.D. and Raman, O. 1987. Melon fruit fly and its control. Indian Horticulture 32: 10-11.
Ahmed, S.O. and Naqvi, S.N.H. 1985. Toxicity and effects of Dimilin on protein pattern of Aedes aegypti. Proc. Entomol. Soc. 14&15: 119-132.
Akhtar, K., Naqvi, S.N.H. and Azmi, M.A. 1987. Insect growth regulating effect of tetranotripenoid (NID) and 3rd instar larvae of Musca domestica. Pak. J. Entomol. 2: 27-33.
Board on Science and Technology for International Development (BOSTID). 1992. Neem: A Tree for solving Global Problem. USA, National Research Council, National Academy Press, Washington D.C. PP.141.
Dhillon, M.K., Singh, R., Naresh, J.S. and Sharma, H.C. 2005. The melon fruit fly, Bactrocera cucurbitae: A review of its biology and management. 16pp. Journal of Insect Science 5: 40, available online: insectscience.org/5.40
Finney, D.J. 1964. Probit Analysis. Cambridge University Press, Cambridge.
Ganesaligan, V.K. 1993. Effects of neem derivatives on larvae of Plutella xylostella in low volume application. Pak. J. Entomol. 8: 47-55.
Jahan, M., Ahmed, I. and Naqvi, S.N.H. 1990. Toxicity of Juliflorine and Margosan-O™ against 3rd instar larvae of Musca domestica L. and its after effect on Morphogenesis. Proc. Pakistan Congr. Zool. 10: 293-300.
Khan, M.F. and Ahmed, S.M. 2000. Toxicity of neem fruit extract & seed oil against Oxycarenus sp. (Heteroptera) pest of cotton crop. Acta Biologica Cracoviensia (Zoologica), Poland. 42: 17-21.
Khan, M.F. and Ahmed, S.M. 2003. Comparative Toxicity of neem fruit extract and Cypermethrin against Tribolium confusum H. and Papilio demoleus L. Philippine J. Sci. 132:109-114.
Khan, M.F., Ahmed. I., Jahan, M., Yasmin, N., Qadri, S.S. and Naqvi, S.N.H. 2007. Toxicological studies of Methoprene (ZR 515) and Dicrotophos as compared to neem extract RB-b and estimation of cholinesterase against Drosophila melanogaster M. J. Exp. Zool., India 10: 121-124.
Khan, M.F., Khan, M.Z., Azmi, M.A. and Qadri, S.S. 1999. An introduc- tion to teratogenic effects of gamma radiation for fruit flies control. Al-Hayat 4: 53-54.
Munir, A., Yasmin, N., Tabassum, R., Naqvi, S.N.H. and Tasneem, S. 1997. Toxicity of SDS and BF (plant extracts) in comparison with Cypermethrin against 3rd instar larvae of citrus butterfly. Pakistan J. Entomol., Karachi. 12: 23-28.
Naqvi, S.N.H. 1996. Prospects and development of a neem based pesticide in Pakistan. Proc. Pakistan Congr. Zool. 16: 325-338.
Naqvi, S.N.H. and Aslam, M. 1996. Phagodeterrent effects of neem preparation against grasshoppers. Proc. Pak. Congr. Zool. 16: 33-36.
Naqvi, S.N.H. and Schmidt, G.H. 1993. Comparative effect of three neem fractions (tetranortriterpenoids) on Musca domestica L. larvae. Pakistan J. Entomol., Karachi. 8: 5-14.
Naqvi, S.N.H. and Tabassum, R. 1992. Probable development of resistance against neem extracts (RB-a) and Cyfluthrin (Solfac 10%) in Musca domestica L. (PCSIR strain). Pakistan J. Entomol., Karachi. 7: 9-16.
Naqvi, S.N.H., Nurulain, S.M., Azmi, M.A. and Asdaque, S.T. 1989. Effect of neem fractions and malathion against white flies Aleurobus barodensis on bringal crop Solanum melongena. Sarhad J. Agric. 5: 25-28.
Naqvi, S.N.H., Rani, S., Tabassum, R. and Ahmed, I. 1996a. Toxicity of neem extracts (RB-a & RB-b) in comparison with Azodrin, Birlane and Cyfluthrin against Dacus cucurbitae (Diptera: Tephritidae). Pakistan. J. Pl. Sci. 2: 209-214.
Naqvi, S.N.H., Raza, S. and Khan, M.F. 1992. Toxicity of neem extract and susceptibility of different populations of Musca domestica against Malathion and DDT. Zoologica Pakistan. 4: 43-50.
Naqvi, S.N.H., Tabassum, R. and Khan, M.F. 1999. Efficacy of neem formulations against susceptible and resistant strains of Musca domestica in comparison with conventional pesticides. Pak. J. Entomol. 14: 9-12.
Naqvi, S.N.H., Tabassum, R. and Khan, M.Z. 1996b. Toxicity determination of neem compounds (NfC & NC) and Dimilin by two methods against Callosobrochus analis. 2nd Entomol. Congr., Islamabad. (1996).
Naqvi, S.N.H., Temuri, K.H., Nurulain, S.M. and Tabassum, R. 1994. Toxicity and IGR effects of neem fractions in Aedes aegypti (PCSIR strain). Pak. J. Entomol. 9: 83-90.
Naqvi, S.N.H., Temuri, K.H., Nurulain, S.M., Ahmed, I. and Larson, R.O. 1995. Toxicity and effect of neem fractions (RBU-9, RB-b and Margosan-O™) on Phosphatases and Protein Patterns of Culex fatigans (K.U. strain). Pakistan. J. Pharm. 12: 49-52.
Nurulain, S.M., Naqvi, S.N.H. and Tabassum, R. 1994. Synergistic formulation of neem product and its IGR effect on Musca domestica L. (PCSIR Strain). Pakistan J. Entomol. Karachi. 9: 43- 50.
Nurulain, S.M., Tabassum, R. and Naqvi, S.N.H. 1989. Toxicity of neem fractions and Malathion against Oxycarenus lugubris Motsch (Dusky cotton bug) wild strain (Heteroptera: Lygaeidae). Pakistan J. Entomol., Karachi 4: 13-24.
Nurulain, S.M., Tabassum, R., Naqvi, S.N.H., Khan, M.Z. and Khan, M.F. 1997. Change in nucleic acids (RNA & DNA) contents of Musca domestica L. under the effects of neem Compounds and DDVP. Pakistan J. Pharmacol. 14: 57-62.
Rizvi, S.A., Yasmin, N., Zaidi, R.H. and Ahmed, S.O. 1986. Toxicity and effects of juvenile hormone analogue on the protein patterns of Oxyrhachis taranada. Pak. J. Entomol. 1: 7-16.
Schmutterer, H. 1995. The Neem tree. pub: VCH New York, Weinheim, U.S.A., Japan, & U.K.
Sharma, H.C., Leuschner, K., Sankaram, A.V.B., Gunaseshar, D., Marthandamurthi, M., Shaskariah, K., Subramanyan, M. and Sultana, N. 1984. Insect antifeedants and growth inhibitors from Azadirachta indica. Proc. 2nd Int. Neem Conf. (Rauischholzhausen 1983) GTZ, Eschborn, Germany. pp. 291-320.
Sombatsiri, K. and Temboonkeat, K. 1987. Efficacy of an improved neem kernel extract in the control of Spodoptera litura and Plutella xylostella in laboratory conditions and field trials. Proc. 3rd Int. Neem Conf. Nairobi 1986, pp. 195-204.
Sombatsiri, K. and Tigvattanont, S. 1984. Effects of neem extracts on some insect pests of economic importance in Thailand. Proc. 2nd Int. Neem Conf. 1983, pp. 95-100.
Tabassum, R., Jahan, M. and Naqvi, S.N.H. 1994. Determination of toxicity of Malathion and RB-a, neem extract against Tribolium castaneum adult. Neem Newsletter 11: 7-9.
Vargas, R.I. and Carey, J.R. 1990. Comparative survival and demo- graphic statistics for wild oriental fruit fly, Med fruit fly and melon fruit fly on papaya J. Econ. Entomol. 83: 1344-1349.
Vollinger, M. 1987. A possible development of resistance against neem seed kernel extract and deltamethrin in Plutella xylostella. 3rd Proc. Int. Neem. Conf. Nairobi 543-54.
Vollinger, M. 1992. Untersuchungen zur resistentzbildung gegen insektizide wirkstoffe des niem baumes Azadirachta indica A. juss im verleich mit synthetischen insektiziden bei zucht populationen der kohlmotte. Plutella xylostella L. (Lepidoptera, Plutellidae). PhD thesis, Univ. Giessen, Germany.
Vollinger, M. 1995. Studies of the probability of development of resistance of Plutella xylostella to neem products, In: The Neem tree, ed: Schmutterrer, H. pp. 477-483., pub: V.H.C., Weinheim, U.S.A., Japan, & U.K.
Yasmin, N., Jafri, S.M.H., Khan, M.F., Nurulain, S.M., Asdaque, T.S. and Naqvi, S.N.H. 1994. Toxic effects of Cyfluthrin on protein patterns of American cockroach. Pak. J. Entomol. 9: 79-82
Yasmin, N., Khan, M.F., Jahan, M., Naqvi, S.N.H. and Tabassum, R. 1995. Toxicological studies of Trichlorfon (OP-insecticide) and RB-a (neem extract) against adults of Drosophila melanogaster (Diptera: Drosophilidae) Pakistan. J. Entomol. Karachi. 10: 65- 68.