Determination of some biochemical parameters of worker honeybees (Apis mellifera L.) belonging to different age groups

The catalase activity, total protein, and total RNA levels of worker bees of different age groups (4, 9, 15, 20, and 24 days old) within the same honeybee colony were studied. The catalase activities of group I (4 days), group II (9 days old), group III (15 days old), group IV (20 days old), and group V (24 days old) were 4.10 ± 0.59, 4.32 ± 0.57, 4.32 ± 0.57, 4.41 ± 0.49, and 4.47 ± 0.48 kU/g, respectively. There were significant differences in total protein and total RNA levels among the different age groups of workers. The calculated total protein levels of group I, group II, group III, group IV, and group V were 22.28 ± 0.77, 21.53 ± 0.59, 20.95 ± 1.05, 18.73 ± 0.93, and 18.24 ± 1.83 g/dL, respectively. The total RNA levels of group I, group II, group III, group IV, and group V were 22.42 ± 0.16, 21.26 ± 0.12, 19.30 ± 0.08, 16.22 ± 0.08, and 11.37 ± 0.07 &mu;g/&mu;L, respectively. The results show that there was no significant difference among the average values of all age groups in catalase activity (P > 0.05), but significant differences were calculated among the total protein values of all age groups (P < 0.05).

Determination of some biochemical parameters of worker honeybees (Apis mellifera L.) belonging to different age groups

The catalase activity, total protein, and total RNA levels of worker bees of different age groups (4, 9, 15, 20, and 24 days old) within the same honeybee colony were studied. The catalase activities of group I (4 days), group II (9 days old), group III (15 days old), group IV (20 days old), and group V (24 days old) were 4.10 ± 0.59, 4.32 ± 0.57, 4.32 ± 0.57, 4.41 ± 0.49, and 4.47 ± 0.48 kU/g, respectively. There were significant differences in total protein and total RNA levels among the different age groups of workers. The calculated total protein levels of group I, group II, group III, group IV, and group V were 22.28 ± 0.77, 21.53 ± 0.59, 20.95 ± 1.05, 18.73 ± 0.93, and 18.24 ± 1.83 g/dL, respectively. The total RNA levels of group I, group II, group III, group IV, and group V were 22.42 ± 0.16, 21.26 ± 0.12, 19.30 ± 0.08, 16.22 ± 0.08, and 11.37 ± 0.07 &mu;g/&mu;L, respectively. The results show that there was no significant difference among the average values of all age groups in catalase activity (P > 0.05), but significant differences were calculated among the total protein values of all age groups (P < 0.05).

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  • Aebi H (1984). Catalase in vitro assay methods. Method Enzymol 105: 121–126.
  • Chomzynski P, Sacchi N (1987). Single-step method of RNA isolation by acid guanidinium thiocyanatephenol–chloroform extraction. Anal Bio Chem 162: 156–159.
  • Corona M, Hughes KA, Weaver DB, Robinson GE (2005). Gene expression patterns associated with queen honey bee longevity. Mech Ageing Dev 126: 1230–1238.
  • Lowry O, Rosebrough NJ, Farr AL, Randall RJ (1951). Protein measurements with the Folin phenol reagent. J Biol Chem 193: 265–275.
  • Orr WC, Sohal RS (1992). The effects of catalase gene overexpression on life span and resistance to oxidative stress in transgenic Drosophila melanogaster. Arch Biochem Biophys 297: 35–41.
  • Peiren N, Graaf DC, Vanrobaeys F, Danneels EL, Devreese B, Van Beeumen J, Jacobs FJ (2008). Proteomic analysis of the honey bee worker venom gland focusing on the mechanisms of protection against tissue damage. Toxicon 52: 72–83.
  • Peters L, Zhu-Salzman K, Pankiw T (2010). Effect of primer pheromones and pollen diet on the food producing glands of worker honey bees (Apis mellifera L.). J Insect Phys 56: 132– 137.
  • Strachecka A, Krauze M, Olszewski K, Borsuk G, Paleolog J, Merska M, Chobotow J, Bajda M, Grzywnowicz K (2014a). Unexpectedly strong effect of caffeine on the vitality of western honeybees (Apis Mellifera). Biochemistry 79: 1192–1201.
  • Strachecka A, Olszewski K, Paleolog J, Borsuk G, Bajda M (2014b). Coenzyme Q10 treatments influence the lifespan and key biochemical resistance systems in the honeybee, Apis mellifera. Arch Insect Biochem Physiol 86: 165–179.
  • Taylor DJ, Robinson GE, Logan BJ, Laverty R, Mercer AR (1992). Changes in brain amine levels associated with the morphological and behavioural development of the worker honeybee. J Comp Physiol A 170:715–721.
  • Wegener J, Huang ZY, Lorenz MW, Bienefeld K (2009). Regulation of hypopharyngeal gland activity and oogenesis in honey bee (Apis mellifera) workers. J Insect Phys 55: 716–725.
  • Weirich GF, Collins AM, Williams VP (2002). Antioxidant enzymes in the honey bee, Apis mellifera. Apidology 33: 3–14.
  • Zhang L, Li J, Wu (2007). Profile analysis of the proteome of the egg of the high royal jelly producing bees (Apis mellifera L.). Agri Sci Chi 6: 1138–1148.
Turkish Journal of Zoology-Cover
  • ISSN: 1300-0179
  • Yayın Aralığı: Yılda 6 Sayı
  • Yayıncı: TÜBİTAK
Sayıdaki Diğer Makaleler

Determination of some biochemical parameters of worker honeybees (Apis mellifera L.) belonging to different age groups

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