In this study the reference ranges belonging to some hematological parameters of Myotis myotis and Myotis blythii, collected from three localities in Turkey in 2012 and 2013, are given for the first time. Bats were caught using a special bat net and they were released after the required amount of blood sample was taken. In Myotis myotis, erythrocyte diameter was recorded as 5.04 μm and the number of erythrocytes and leukocytes in 1 mm3 of blood was 11.35 × 106 and 4865, respectively. While the hemoglobin level was 14.9 g/dL, the hematocrit value was measured as 57.0%. The other values were as follows: mean cell volume (MCV) 52.40 fL, mean cell hemoglobin (MCH) 13.70 pg, mean cell hemoglobin concentration (MCHC) 23.30%, and amount of total protein 10.5 g/L. In Myotis blythii specimens, erythrocyte diameter was recorded as 5.69 μm and the number of erythrocytes and leukocytes in 1 mm3 of blood was 14.52 × 106 and 4296, respectively. While the hemoglobin level was 13.09 g/dL, the hematocrit value was measured as 60.20%. The other values measured as follows: MCV 43.67 fL, MCH 9.70 pg, MCHC 21.70%, and amount of total protein 10.625 g/L.