The Effects of in Ovo Insulin-Like Growth Factor-1 on Embryonic Development of Musculus Longus Colli Dorsalis in Japanese Quail

The objective of the present study was to evaluate the effects of insulin-like growth factor-1 (IGF-1) on the histological development of the thoracic part of the m. longus colli dorsalis (m. spinalis thoracis) in Japanese quail embryos. A single in ovo dose of recombinant human IGF-1 (rhIGF-1) (100 ng embryo-1) was administered through the blunt end of eggs via a single hole made with a dental drill bit, without penetrating the chorioallantoic membrane. For histological evaluation, the embryos were collected daily from days 7 to 16 of embryonic development (E). In ovo administration of rhIGF-1 increased the diameter of muscle fibers on E7, 9, 10, 11, and 13. Additionally, in ovo rhIGF-1 also increased the number of muscle fibers (P < 0.001). It was concluded that rhIGF-1 accelerated skeletal muscle development in the quail embryos.

Anahtar Kelimeler:

Quail, skeletal muscle development, rhIGF-1, in ovo

The Effects of in Ovo Insulin-Like Growth Factor-1 on Embryonic Development of Musculus Longus Colli Dorsalis in Japanese Quail

Keywords:

Quail, skeletal muscle development, rhIGF-1, in ovo,

PDF

___

Dodson, M.V., Allen, R.E., Hossner, K.L.: Ovine somatomedin, multiplication-stimulating activity, and insulin promote skeletal muscle satellite cell proliferation in vitro. Endocrinology, 1985; 117: 2357-2363.
Girbau, M., Gomez, J.A., Lesniak, M.A., de Pablo F.: Insulin and insulin-like growth factor-1 both stimulate metabolism, growth and differentiation in the post-neurula chick embryo. Endocrinology, 1987; 121:1477-1482.
Kocamis, H., Kirkpatrick-Keller, D.C., Klandorf, H., Killefer, J.: In ovo administration of recombinant human insulin-like growth factor-1 (rhIGF-1) alters postnatal growth and development of the broiler chicken. Poult. Sci., 1998; 77: 1913-1919.
Luna, L. G.: Manual of Histologic Staining Methods of the Armed Forces Institute of Pathology. New York, McGraw-Hill Book Comp, 3rd. ed., 72-100, 1968.
Minitab: Minitab Reference Manual. Release 10 for Windows Minitab Inc., USA, 1994.
Kabukçu, M.A.: Sağlık Sosyal ve Fen Bilimlerinde Uygulamalı İstatistik. Selçuk Üniv. Ziraat Fak., Tarım Ekonomi Bölümü, Konya, 1994.
McQueeney, K., Dealy, C.N.: Roles of insulin-like growth factor-I (IGF-I) and IGF-I binding protein-2 (IGFBP2) and -5 (IGFBP5) in developing chick limbs. Growth Horm IGF Res. 2001; 11: 346- 363.
Hamburger, V., Hamilton, H.L.: A. Series of normal stages in the development of chick embryo. J. Morphol., 1951; 88: 49-92.
Grim, M.: Differentiation of myoblasts and relationship between somites and the wing bud of the chick embryo. Z. Anat. Entwicklungsgesch., 1970; 132: 260-271.
Sweeney, L.J., Kenndey, J.M., Zak, R., Kokjohn, K., Kelly, S.W.: Evidence for expression of a common myosin heavy chain phenotype in future fast and slow skeletal muscle during initial stages of avian embryogenesis. Dev. Biol. 1989; 133: 361-374.
Michael, M.I., El-Mekkawy, D.A., Ibrahim, L.L., El-Zayat, M.A., El- Wakil, A.A.: Development of the wing in embryonic stages of the chick and quail 11-histological studies. J. Egypt. Ger. Soc. Zool., 1998; 25: 83-111.
O’Neill, M.C.: Growth and differentiation during myogenesis in the chick embryo. Dev. Biol. 1987; 120: 465-480.
McDonald, K.A., Lakonishok, M., Horwitz, A.F.: Alpha v and alpha 3 integrin subunits are associated with myofibrils during myofibrillogenesis. J. Cell Sci., 1995; 108: 2573-2581.
George, J.C., Berger, A.J.: Avian Myology. Academic Press, New York, 25-110, 1966.
Hodges, R.D.: The Histology of the Fowl. Academic Press, New York, 243-295, 1974.
Sağlam, M., Aştı, R. N., Özer, A.: Genel Histoloji. Yorum Matbaacılık, Ankara, 6. Baskı, 245-274, 2001.
Murray, R.K., Mayes, P.A., Granner, D.K., Rodwell V.W.: Harper’ın Biyokimyası. Barış Kitabevi, İstanbul, 595-614, 1993.
Van Wyk, J.J.: The somatomedins: Biological actions and physiological control mechanisms. In: Li, C.H. Ed. Hormonal Proteins and Peptides. Academic Press, New York, 82-125, 1984.
Vasilatos-Younken, R., Scanes, C.G.: Growth hormone and insulin-like growth factors in poultry growth: required, optimal or ineffective? Poult. Sci., 1991; 70: 1764-1780.
Kikuchi, K., Buonomo, F.C., Kajimoto, Y., Rotwein, P.: Expression of insulin-like growth factor-1 during chicken development. Endocrinology, 1991; 128: 1323-1328.
Mathews, L.S., Hammer, R.E., Behringer, R.R., D'Ercole, A.J., Bell, G.I., Brinster, R.L., Palmiter, R.D.: Growth enhancement of transgenic mice expressing human insulin-like growth factor1. Endocrinology. 1988; 123: 2827-2833.