Öznur ASLAN, Aytaç AKÇAY, İlknur BEKDİK KARACA, Ömer Orkun DEMİRAL, Kadir Semih GÜMÜŞSOY

Seroprevalence of caseous lymphadenitis in Kangal Akkaraman sheep

In this study, the seroprevalence of Corynebacterium pseudotuberculosis, the causative agent of caseous lymphadenitis (CLA)infection, in Kangal Akkaraman sheep flocks was examined. For this purpose, blood samples were collected from 25 different sheep flocks (n = 379). According to the ELISA results, the numbers of CLA-positive and CLA-negative samples were 138 (36.4%) and 241 (63.6%), respectively. CLA positivity was observed in 35.7% of the females (n = 241), 37.7% of the males (n = 138), 40.2% of the sheep with clinical symptoms (n = 184), and 32.8% of the sheep with no clinical symptoms (n = 195) (P > 0.05). We concluded that C. pseudotuberculosis is a disease observed widely in the Kangal Akkaraman sheep breed in Turkey. To the best of our knowledge, this is the first study reporting the seroprevalence of CLA in Kangal Akkaraman sheep, a breed whose first official herdbook was only recently established.

PDF

___

1. Yerli Hayvan Irk ve Hatlarının Tescili Hakkında Tebliğ (Tebliğ No: 2004/39)de Değişiklik Yapılmasına Dair Tebliğ (Tebliğ No: 2012/61) T.C. Resmi Gazete, 28384, 14 August 2012 (in Turkish).
2. Fontaine MC, Baird GJ. Caseous lymphadenitis. Small Rumin Res 2008; 76: 42-48.
3. Dorella FA, Pacheco LG, Oliveira SC, Miyoshi A, Azevedo V. Corynebacterium pseudotuberculosis: microbiology, biochemical properties, pathogenesis and molecular studies of virulence. Vet Res 2006; 37: 201-218.
4. Baird GJ, Fontaine MC. Corynebacterium pseudotuberculosisand its role in ovine caseous lymphadenitis. J Comp Pathol 2007; 137: 179-210.
5. Batey RG. The effect of caseous lymphadenitis on body condition and weight of Merino mutton carcasses. Aust Vet J 1986; 63: 268.
6. Alonso JL, Simon MC, Girones O, Musquiz JL, Ortega C, Garcia J. The effect of experimental infection with Corynebacterium pseudotuberculosis on reproduction in adult ewes. Res Vet Sci 1992; 52: 267-272.
7. Paton MW, Walker SB, Rose IR, Watt GF. Prevalence of caseous lymphadenitis and usage of caseous lymphadenitis vaccines in sheep flocks. Aust Vet J 2003; 81: 91-95.
8. İlhan Z, Akan M, İzgur M, Akay Ö, Keçeli H. Detection of antibodies elicited to Corynebacterium pseudotuberculosisin sheep by hemolysis inhibition test. Veteriner Hekimleri Mikrobiyoloji Dergisi Elektronik Versiyonu 2001; 1: 1-9 (in Turkish with English abstract).
9. Ribeiro D, Dorella FA, Pacheco LGC, Seyffert N, Castro TLP, Portela RWD, Meyer R, Miyoshi A, Luvizotto MCR, Azevedo V. Subclinical diagnosis of caseous lymphadenitis based on ELISA in sheep from Brazil. J Bacteriol Parasitol 2013; 4: 1-4.
10. Stoops SG, Renshaw HW, Thilsted JP. Ovine caseous lymphadenitis: disease prevalence, lesion distribution, and thoracic manifestations in a population of mature culled sheep from western United States. Am J Vet Res 1984; 45: 557-561.
11. Zavoshti RF, Khoojine ABS, Helan JA, Hassanzadeh B, Heydari AA. Frequency of caseous lymphadenitis in sheep slaughtered in an abattoir in Tabriz: comparison of bacterial culture and pathological study. Comp Clin Path 2012; 21: 667-671.
12. Çetinkaya B, Karahan M, Atil E, Kalin R, De Baere T, Vaneechoutte M. Identification of Corynebacterium pseudotuberculosis isolates from sheep and goats by PCR. Vet Microbiol 2002; 88: 75-83.
13. Aydın N. Corynebacterium pseudotuberculosis ovis suşlarının ve ekzotoksinlerinin antijenik özellikleri üzerinde araştırmalar. PhD, Ankara University, Ankara, Turkey, 1977 (in Turkish).
14. Erganiş O, Kaya O, Ateş M, İstanbulluoğlu E. Konya EBK kombinasında kesilen koyunlardaki apseli lenf yumruları üzerine mikrobiyolojik ve serolojik incelemeler. Veterinarium 1990; 1: 8-11 (in Turkish).
15. Muz A, Eröksüz K, Öngör R, Ertaş HB, Kaya A, Kalender H. Elazığ Et ve Balık Kurumu mezbahasında kesilen koyunlardaki apseli preskapular lenf yumruları zerinde mikrobiyolojik, serolojik ve patolojik incelemeler. Fırat Üniversitesi Sağlık Bilimleri Dergisi 1995; 9: 204-211 (in Turkish).
16. Paton MW. Caseous lymphadenitis. In: Proceedings of the Fourth International Congress for Sheep Veterinarians; Armidale, Australia; 1997. p. 121.
17. Pépin M, Pardon P, Marly J, Lantier F. Corynebacterium pseudotuberculosis infection in adult ewes by inoculation in the external ear. Am J Vet Res 1988; 49: 459-463.
18. Saunders VF, Redacliff LA, Berg T, Hornitzky M. Multiplex PCR for the detection of Brucella ovis, Actinobacillus seminisand Histophilus somni in ram semen. Aust Vet J 2007; 85: 72-77.
19. Al-Gaabary MH, Osman SA, Oreiby AF. Caseous lymphadenitis in sheep and goats: Clinical, epidemiological and preventive studies. Small Ruminant Res 2009; 87: 116-121.
20. Batey RG, Speed CM, Kobes CJ. Prevalence and distribution of caseous lymphadenitis in feral goats. Aust Vet J 1986; 63: 33-36.
21. Renshaw HW, Graff VP, Gates NL. Visceral caseous lymphadenitis in thin ewe syndrome: isolation of Corynebacterium, Staphylococcus and Maraxella spp. from internal abscesses in emaciated ewes. Am J Vet Res 1979; 40: 1110-1114.
22. Ellis TM, Sutherland SS, Wilkinson FC, Mercy AR, Paton MW. Role of lung Corynebacterium pseudotuberculosis lesions in the transmission of this bacterium to other sheep. Aust Vet J 1987; 64: 261-263.
23. Robertson JP. Studies on diagnosis, epidemiology and immunity of Corynebacterium pseudotuberculosis infection in sheep. MPhil, Murdoch University, Murdoch, Australia, 1980.
24. İzgür M, Akan M, İlhan Z. Microorganisms isolated from cases of caseous lymphadenitis. Ankara Üniversitesi Veteriner Fakültesi Dergisi 1999; 46: 43-50.
25. Saeed EMA, Alharbi KB. Morels disease and caseous lymphadenitis: a literature review with special reference to Saudi Arabia. IOSR Journal of Agriculture and Veterinary Science 2014; 7: 76-86.