Samikshya SARANGI, Anuradha GUPTA, Neelam BANSAL, Varinder UPPAL

Seasonal variations in histomorphology and histochemistry of the prostate gland of buffalo bulls

The prostate glands of buffalo bulls were collected from an abattoir immediately after slaughter and were fixed in 10% neutral buffered formalin for paraffin block preparation by an acetone-benzene schedule. Paraffin sections of 3-5 μm were obtained on glass slides with a rotary microtome and were stained with various histomorphological and histochemical stains. The distributions of glandular acini and ducts were variable throughout the length of the pelvic urethra. The disseminate part of the prostate gland was derived from the epithelium of the pelvic urethra and was a compound tubulo-alveolar type of gland. The glandular acini comprised three types of cells, i.e. principal (serous cells), mucous, and narrow dark cells. In winter, the lumen of the acini became wider than in summer. The amount of glandular tissue decreased from winter to summer, whereas a reverse pattern was observed in the stroma. The prostatic concretions were larger and centrally placed during winter but became peripheral in summer. The average epithelial height of prostatic acini varied significantly between seasons. The activity of neutral mucopolysaccharides, basic proteins, lipids, and phospholipids was higher in winter than summer, whereas that of acid mucopolysaccharides was higher in summer than winter.

Keywords:

Prostate histomorphology, histochemistry, seasonal, buffalo,

PDF

___

1. Taneja VK, Birthal PS. Role of buffalo in food security in Asia. Asian Buffalo Magazine 2004; 1 (1): 4-13.
2. Abou-Elhamd AS, Salem AO, Selim AA. Histomorphological studies on the prostate gland of donkey Equus asinus during different seasons. Journal of Histology 2013; 2013: 643287.
3. Chopra R. Post-natal development of the prostate gland in buffalo (Bubalus bubalis): A histomorphochemical study. MVSc, Punjab Agricultural University, Ludhiana, Punjab, India, 1998.
4. Eurell J, Frappier BL. Dellmann’s Textbook of Veterinary Histology. 6th ed. New York, NY, USA: Wiley-Blackwell; 2006.
5. Suri S. Gross, Histological and histochemical changes in male accessory genital glands of Gaddi goat and Gaddi sheep - A seasonal study. PhD, Chaudhary Sarwan Kumar Himachal Pradesh Krishi Vishwavidyalaya, Palampur, Himachal Pradesh, India, 2007.
6. Luna LG. Manual of Histologic Staining Methods of the Armed Forces Institute of Pathology. 3rd ed. New York, NY, USA: McGraw-Hill Book Company; 1968.
7. Sheehan DC, Hrapchak BB. Theory and Practice of Histochemistry. Saint Louis, MO, USA: CV Mosby Co.; 1973.
8. Chayen J, Butcher RG, Bitensky L, Poulter LW. A Guide to Practical Histochemistry. Edinburgh, UK: Oliver and Boyd; 1969.
9. Snedecor GW, Cochran WG. Statistical methods. 9th ed. Ames, IA, USA: Iowa State University Press; 1994.
10. Adhikary GN, Begum MIA, Islam MN, Islam KM, Rauf SMA. Morpho-histometric evaluations of pre-pubertal, pubertal and post pubertal prostate glands of indigenous bulls (Bos indicus) of Bangladesh. International Journal of Biological & Pharmaceutical Research 2015; 6 (8): 586-592.
11. Sudhakar LS. Histological and histochemical changes in the male accessory genital glands of Murrah buffalo during postnatal development. PhD, Haryana Agricultural University, Hisar, India, 1982.
12. Roy KS, Pawar HS, Saigal RP. Histomorphological, histochemical and histoenzymological studies on prostate gland of ram (Ovis aries). Indian Journal of Animal Science 1985; 55 (12): 983-986.
13. Pathak A, Katiyar RS, Sharma DN, Farooqui MM, Prakash A. Gross anatomical, histological and histochemical studies on the postnatal development of the prostate gland of Gaddi goat. International Journal of Morphology 2012; 30 (2): 731-739.
14. Chandramouly KN. Studies on the histology of prostate in the Indian buffalo (Bos bubalis). Mysore Journal of Agricultural Sciences 1971; 5: 32-38.
15. Chandrapal S. Gross histological and histochemical studies on the male genital system of buffalo (Bubalus bubalis). PhD, Agra University, Agra, Uttar Pradesh, India, 1976.
16. Sambyal RS. Histomorphological and histochemical studies on the accessory genital glands of male camel (Camelus dromedarius). MVSc, Haryana Agricultural University, Hisar, India, 1989.
17. Guraya SS. Biology of Ovarian Follicles in Mammals. Berlin, Germany: Springer Verlag; 1985.
18. Gupta AN, Singh Y. Histological and histochemical studies on the prostate gland of goat. Indian Journal of Animal Science 1982; 52: 82-89.
19. Pyne SK, Sinha RD, Chauhan HVS. Histochemical study on the bulbo-urethral gland of normal and vasectomized goats (Capra hircus). Indian Veterinary Journal 1991; 68: 145-147.