Gözde KONURAY, Zerrin ERGİNKAYA, Zerrin ALKAN, Emel ÜNAL TURHAN

Production of biosurfactant by lactic acid bacteria using whey as growth medium

The aim of this study was to produce biosurfactants from whey waste using Streptococcus thermophilus, Lactobacillusacidophilus, and Lactobacillus rhamnosus as well as to determine oil spreading, emulsification index, surface tension, and antiadhesiveproperties in these biosurfactants. Additionally, the capability of biosurfactant production from whey waste in the dairy industry wascompared with that of MRS broth, a commercial culture medium. The presence of biosurfactants by all lactic acid bacteria was detectedusing the oil spreading test. Zone diameter due to the surface activity of lactic acid bacteria strains ranged from 1.87 to 5.92 cm.Biosurfactants from both whey medium and MRS broth reduced surface tension. Differences between data from whey medium and MRSbroth were statistically insignificant in terms of the biomass, oil spreading, and surface tension of biosurfactants. Emulsification indexvalues recorded after 1 h, 24 h, and 1 week were significantly different and ranged from 19.50% to 58.00%. The highest emulsificationactivity was exhibited by L. acidophilus from whey medium in the first hours. A 10 mg/mL concentration of biosurfactants was ableto prevent S. aureus, P. aeruginosa, and E. coli adhesion 37.25%–52.5%, 10.25%–23.25%, and 5.32%–11.50%, respectively. E. coli wasmore resistant to the biosurfactants than the other pathogens were. On the other hand, biosurfactants from L. rhamnosus had the lowestantiadhesive effects. In general, biosurfactants from whey medium and MRS broth were similar in terms of antiadhesion properties.The present study showed that dairy wastes could be an appropriate medium for cost-effective biosurfactant production by lactic acidbacteria for the benefit of the food, pharmaceutical, and cosmetic industries.

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1. Fariq A, Saeed A. Production and biomedical applications of probiotic biosurfactants. Current Microbiology 2016; 72: 489- 495. doi: 10.1007/s00284-015-0978-4
2. Dubey K, Juwarkar A. Distillery and curd whey wastes as viable alternative sources for biosurfactant production. World Journal of Microbiology & Biotechnology 2001; 17: 61-69.
3. Dubey K, Juwarkar A. Determination of genetic basis for biosurfactant production in distillery and curd whey wastes utilizing Pseudomonas aeruginosa strain in BS2. Indian Journal of Biotechnology 2004; 3: 74-81.
4. Campos JM, Stamford TLM. Microbial biosurfactants as additives for food industries. American Institute of Chemical Engineers 2013; 29 (5): 1097-1107. doi: 10.1002/btpr.1796
5. Kaur S, Kaur P, Nagpal R. In vitro biosurfactant production and biofilm inhibition by lactic acid bacteria isolated from fermented food products. International Journal of Probiotics and Prebiotics 2015; 10 (1): 17-22.
6. Gudina EJ, Teixeira JA, Rodrigues LR. Biosurfactantproducing lactobacilli: screening, production profiles, and effect of medium composition. Applied and Environmental Soil Science 2011; 201254: 1-9. doi: 10.1155/2011/201254
7. Gomaa EZ. Antimicrobial activity of a biosurfactant produced by Bacillus licheniformis strain M104 grown on whey. Brazilian Archives of Biology and Technology 2013; 56 (2): 259-268.
8. Makkar RS, Cameotra, Banat IM. Advances in utilization of renewable substrates for biosurfactant production. AMB Express 2011; 1(5): 1-19. doi: 10.1186/2191-0855-1-5
9. Panesar PS, Kennedy JF. Biotechnological approaches for the value addition of whey. Critical Review of Biotechnology 2012; 32 (4): 327-348. doi: 10.3109/07388551.2011.640624
10. Golek P, Bednarski W, Lewandowska M. Characteristics of adhesive properties of Lactobacillus strains synthesizing biosurfactants. Polish Journal of Natural Science 2007; 22 (2): 333-342. doi: 2478/v10020-007-0031-6
11. Golek P, Bednarski W, Brzozowski B, Dziuba B. The obtaining and properties of biosurfactants synthesized by bacteria of the genus Lactobacillus. Annals of Microbiology 2009; 59 (1): 119- 126.
12. Fracchia L, Cavallo M, Allegrone G, Martinotti MG. A Lactobacillus-derived biosurfactant inhibits biofilm formation of human pathogenic Candida albicans biofilm producers. In: Mendez-Vilas A (editor). Current Research, Technology and Education Topics in Applied Microbiology and Microbial Biotechnology: Microbiology Series NO2, Volume 1. Badajoz, Spain: Formatex; 2010. pp. 827-837.
13. Kermanshahi RK, Peymanfar S. Isolation and identification of Lactobacilli from cheese, yoghurt and silage by 16s rDNA gene and study of bacteriocin and biosurfactant production. Jundishapur Journal of Microbiology 2012; 5 (4): 528-532. doi: 10.5812/jjm.3444
14. Cornea CP, Roming FI, Sicuia OA, Voaides C, Zamfir M et al. Biosurfactant production by Lactobacillus spp. strains isolated from Romanian traditional fermented food products. Romanian Biotechnological Letters 2016; 21 (2): 11312-11320.
15. Gomes MZ, Nitschke M. Evaluation of rhamnolipid and surfactin to reduce the adhesion and remove biofilms of individual and mixed cultures of food pathogenic bacteria. Food Control 2012; 25: 441-447. doi: 10.1016/j.foodcont.2011.11.025
16. Tarım Orman ve Köy İşleri Bakanlığı. Gıda Maddeleri Muayene ve Analiz Yöntemleri. Tarım Orman ve Köy İşleri Bakanlığı, Gıda İşleri Genel Müdürlüğü, Genel Yayın No: 62, Ankara, Turkey: 1983 (in Turkish).
17. AOAC. Official Methods of Analysis. 15th ed. Washington DC, USA: Association of Official Analytical Chemists, 1990.
18. Rodrigues LR, Moldes A, Teixeira J, Oliveira R. Kinetic study of fermentative biosurfactant production by Lactobacillus strains. Biochemical Engineering Journal 2006; 109-116. doi: 10.1016/j.bej.2005.06.001
19. Rodrigues LR, Teixeira JA, Mei HC, Oliveira R. Physicochemical and functional characterization of a biosurfactant produced by Lactococcus lactis 53. Colloids and Surfaces B: Biointerfaces 2006; 79-86. doi: 10.1016/j.colsurfb.2006.03.003
20. Gudina EJ, Rocha V, Teixeira JA, Rodrigues LR. Antimicrobial and antiadhesive properties of a biosurfactant isolated from Lactobacillus paracasei ssp. paracasei A20. Letters in Applied Microbiology 2010; 50: 419-424. doi: 10.1111/j.1472- 765X.2010.02818.x
21. Augustin M, Hippolyte MT. Screening of biosurfactants properties of cell-free supernatant of cultures of Lactobacillus spp. isolated from a local fermented milk (pendidam) of Ngaoundere (Cameroon). International Journal of Engineering Research and Applications 2012; 2 (5): 974-985.
22. Yin H, Qiang J, Jia Y, Ye J, Peng H et al. Characteristics of biosurfactant produced by Pseudomonas aeruginosa S6 isolated from oil-containing wastewater. Process Biochemistry 2009; 44: 302-308. doi: 10.1016/j.procbio.2008.11.003
23. Youssef NH, Duncan KE, Nagle DP, Savage KN, Knapp RM et al. Comparison of methods to detect biosurfactant production by diverse microorganisms. Journal of Microbiological Methods 2004; 56: 339-347. doi: 10.1016/j.mimet.2003.11.001
24. Karthik L, Kumar G, Rao KVB. Comparison of methods and screening of biosurfactant producing marine actinobacteria isolated from nicobar marine sediment. The IIOAB Journal 2010; 1 (2): 34-38.
25. Sarin S, Khamsri B, Sarin C. Isolation of biosurfactantproducing bacteria with antimicrobial activity against pathogens. Environment Asia 2011; 4 (1): 1-5. doi: 10.14456/ ea.2011.1
26. Ramnani P, Kumar SS, Gupta R. Concomitant production and downstream processing of alkaline protease and biosurfactant from Bacillus licheniformis RG1: bioformulation as detergent additive. Process Biochemistry 2005; 40: 3352-3359. doi: 10.1016/j.procbio.2005.03.056
27. Accorsini FR, Muttan MJR, Lemas EGM, Benincasa M. Biosurfactant production by yeasts using soybean oil and glycerol as low cost substrate. Brazilian Journal of Biotechnology 2012; 116-125. doi: 10.1590/S1517-83822012000100013
28. Mehdi H, Giti E. Investigation of alkane biodegradation using the microtiter plate method and correlation between biofilm formation, biosurfactant production and crude oil biodegradation. International Biodeterioration & Biodegradation 2008; 62: 170-178. doi: 10.1016/j. ibiod.2008.01.004
29. Bek Y, Efe E. Araştırma ve Deneme Metodları. Adana, Turkey: Ç.Ü. Ziraat Fakültesi, 1988 (in Turkish).
30. Rodrigues LR, Teixeira J, Oliveira R. Low-cost fermentative medium for biosurfactant production by probiotic bacteria. Biochemical Engineering Journal 2006; 135-142. doi: 10.1016/j. bej.2006.09.012
31. Rodrigues L, Teixeira J, Oliveira R, Mei HC. Response surface optimization of the medium components for the production of biosurfactants by probiotic bacteria. Process Biochemistry 2006; 41: 1-10. doi: 10.1016/j.procbio.2005.01.030
32. Gudina EJ, Fernandes EC, Teixeira JA, Rodrigues LR. Antimicrobial and anti-adhesive activities of cell-bound biosurfactant from Lactobacillus agilis CCUG31450. The Royal Society of Chemistry 2015; 5: 90960-90968. doi: 10.1039/ c5ra11659g
33. Potowary R, Potowary K, Kalita MC, Deka S. Utilization of paneer whey waste for cost-effective production of rhamnolipid biosurfactant. Applied Biochemistry and Biotechnology 2016; 180: 383-399. doi: 10.1007/s12010-016-2105-9
34. Satpute SK, Mone NS, Das P, Banpurkar AG, Banat IM. Lactobacillus acidophilus derived biosurfactant as a biofilm inhibitor: a promising investigation using microfluidic approach. Applied Science 2018; 8: 1-14. doi: 10.3390/ app8091555
35. Merghni A, Dallel I, Noumi E, Kadmi Y, Hentati H et al. Antioxidant and antiproliferative potential of biosurfactants isolated from Lactobacillus casei and their anti-biofilm effect in oral Staphylococcus aureus strains. Microbial Pathogenesis 2017; 104: 84-89. doi: 10.1016/j.micpath.2017.01.017
36. Vera ECS, Azevedo POS, Dominguez JM, Oliveira RPS. Optimization of biosurfactant and bacteriocin-like inhibitory substance (BLIS) production by Lactococcus lactis CECT-4434 from agroindustrial waste. Biochemical Engineering Journal 2018; 133: 168-178. doi: 10.1016/j.bej.2018.02.011
37. Gomaa EZ. Antimicrobial and anti-adhesive properties of biosurfactant produced by lactobacilli isolates, biofilm formation and aggregation ability. Journal of General Applied Microbiology 2013; 59: 425-436.
38. Rienzo MAD, Stevenson P, Marchant R, Banat IM. Antibacterial properties of biosurfactants against selected gram-positive and -negative bacteria. FEMS Microbiology Letters 2016; 363: 1-8. doi: 10.1093/femsle/fnv224