Pistacia atlantica gum aqueous extract modulates humoral and cellular immune responses in BALB/c mice
Natural herbal products contain different compounds that can modulate immune and inflammatory diseases. This investigation aimed to examine the influence of Pistacia atlantica aqueous extract on cellular and humoral immunity in mice following the exposure to sheep red blood cells (SRBCs). For this purpose, male BALB/c mice (n = 40) were randomly assigned into 4 equivalent groups. The animals received 1 × 109 SRBCs in complete Freund?s adjuvant (CFA) intraperitoneally 3 times with two-week intervals. Five days after the final injection, blood samples were taken from the animals. Additionally, 1 × 109 SRBCs were also injected into the mice?s left hind footpad 48 h before the blood collection. Three groups of mice received various oral doses of the extract (100, 200, and 400 mg/kg) for 52 consecutive days after the study onset. Measurements were performed on the levels of humoral and cellular immune responses, splenocytes? ability to uptake neutral red, respiratory burst, and cell viability. The mice receiving Pistacia atlantica in all concentrations displayed significantly low delayed type hypersensitivity (DTH) reactions in a dose-dependent manner compared to the control animals. The treated mice also presented a dose-dependent, significantly lower average anti-SRBC titer than that in the control animals. IL-17 and IL-10 cytokine levels respectively decreased and increased significantly in the mice that received Pistacia atlantica compared to the control group. Splenocyte proliferation and respiratory burst declined significantly in mice treated with Pistacia atlantica as opposed to the normal control group but phagocytosis ability was significantly decreased. The immunomodulatory impacts of Pistacia atlantica might be partially caused by immunity diversion from proinflammatory cytokine IL-17 into antiinflammatory cytokine IL-10.
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- 1. Soraya H, Moloudizargari M, Aghajanshakeri Sh, Javaherypour
S, Mokarizadeh A et al. Angiogenic effect of the aqueous extract
of Cynodon dactylon on human umbilical vein endothelial cells
and granulation tissue in rat. DARU Journal of Pharmaceutical
Sciences 2015; 29 (23): 10. doi: 10.1186/s40199-015-0093-x
- 2. Laniosz V, Lehman JS, Poland GA, Wetter DA. Literaturebased immunization recommendations for patients requiring
immunosuppressive medications for autoimmune bullous
dermatoses. International Journal of Dermatology 2016; 55 (6):
599-607. doi: 10.1111/ijd.13140
- 3. Gustavsen MT, Midtvedt K, Lønning K, Jacobsen T, Reisæter
AV et al. Evaluation of tools for annual capture of adherence to
immunosuppressive medications after renal transplantation a
single-centre open prospective trial. Transplant International
2019; 32 (6): 614-625. doi: 10.1111/tri.13412
- 4. Dudics S, Langan D, Meka RR. Natural products for the
treatment of autoimmune arthritis: their mechanisms of action,
targeted delivery, and interplay with the host microbiome.
International Journal of Molecular Sciences 2018; 19 (9):
E2508. doi: 10.3390/ijms19092508
- 5. Zamawe C, King C, Jennings HM. Effectiveness and safety of
herbal medicines for induction of labour: a systematic review
and meta-analysis. British Medical Journal Open 2018; 17: 8
(10): e022499. doi: 10.1136/bmjopen-2018-022499
- 6. Jamshidi-Kia F, Lorigooini Z, Amini-Khoei H. Medicinal
plants: past history and future perspective. Journal of Herbmed
Pharmacology 2018; 7 (1): 1-7. doi: 10.15171/jhp.2018.01
7. Xiao Ch, Guan Q, Tan Y. Medical plants and immunological
regulation. Journal of Immunology Research 2018; 2018:
9172096. doi.org/10.1155/2018/9172096
- 8. Fan W, Zheng P, Wang Y. Analysis of immunostimulatory
activity of polysaccharide extracted from Yu-Ping-Feng in vitro
and in vivo. Biomedicine & Pharmacotherapy 2017; 93: 146-
155. doi: 10.1016/j.biopha.2017.05.138
- 9. Khodavaisy S, Rezaie S, Noorbakhsh F. Effects of Pistacia
atlantica subsp. kurdica on growth and aflatoxin production
by Aspergillus parasiticus. Jundishapur Journal of Microbiology
2016; 9 (7): e35452. doi: 10.5812/jjm.35452
- 10. Sharifi M, Hazell SL. GC-MS analysis and antimicrobial activity
of the essential oil of the trunk exudates from Pistacia atlantica
kurdica. Indian Journal of Pharmaceutical Sciences 2011; 3 (2):
1364-1367. doi: 10.1007/s10600-014-0959-z
- 11. Taran M, Mohebali M, Esmaeli J. In vivo efficacy of gum
obtained Pistacia atlantica in experimental treatment of
cutaneous leishmaniasis. Iranian Journal of Public Health 2010;
39 (1): 36-41.
- 12. Mahjoub F, Akhavan Rezayat K, Yousefi M, Mohebbi M, Salari
R. Pistacia atlantica Desf. a review of its traditional uses,
phytochemicals and pharmacology. Journal of Medicine and
Life 2018; (3): 180-186. doi: 10.25122/jml-2017-0055
- 13. Shakarami Z, Esmaeili Gouvrchin Ghaleh H, Mansouri Motlagh
B, Sheikhian A, Jalali Kondori B. Evaluation of the protective
and therapeutic effects of Pistacia atlantica gum aqueous extract
on cellular and pathological aspects of experimental asthma in
Balb/c mice. Avicenna Journal of Phytomedicine 2019; 9 (3):
248-259. doi: 10.22038/AJP.2019.12310
- 14. Tanideh N, Masoumi S, Hosseinzadeh M. Healing effect of
Pistacia atlantica fruit oil extract in acetic acid-induced colitis
in rats. Iranian Journal of Medical Sciences 2014; 39 (6): 522-
528.
- 15. Abtahi Froushani SM, Zarei L, Esmaeili Gouvarchin Ghaleh H,
Mansori Motlagh B. Estragole and methyleugenol-free extract
of Artemisia dracunculus possesses immunomodulatory effects.
Avicenna Journal of Phytomedicine 2016; 6 (5): 526-534.
- 16. Abtahi Froushani SM, Esmaili Gourvarchin Galeh H. New
insight into the immunomodulatory mechanisms of tretinoin
in NMRI mice. Iranian Journal of Basic Medical Sciences 2014;
17 (9): 632-637.
- 17. Esmaili Gourvarchin Galeh H, Abtahi Froushani SM,
Afzale Ahangaran N, Naji Hadai S. Effects of educated
monocytes with xenogeneic mesenchymal stem cell–derived
conditioned medium in a mouse model of chronic asthma.
Immunological Investigations 2018; 47 (5):504-520. doi:
10.1080/08820139
- 18. Jabbari N, Zarei L, Esmaeili Govarchin Galeh H,
Mansori Motlagh B. Assessment of synergistic effect of
combining hyperthermia with irradiation and calcium
carbonate nanoparticles on proliferation of human breast
adenocarcinoma cell line (MCF-7 cells). Artificial Cells, Blood
Substitutes, and Biotechnology 2018; 46 (Suppl. 2): 364-372.
doi: 10.1080/21691401
- 19. Esmaili Gouvarchin Galeh H, Delirezh N, Abtahi Froushani
SM, Afzale Ahangaran N. Calcitriol modulates the effects of
the supernatants of bone-marrow–derived mesenchymal stem
cells on neutrophil functions. Turkish Journal of Biology 2014;
38: 365-370. doi: 10.3906/biy-1310-40
- 20. Askenasy N. Mechanisms of autoimmunity in the non-obese
diabetic mouse: effector/regulatory cell equilibrium during
peak inflammation. Immunology 2016; 147 (4): 377-88. doi:
10.1111/imm.12581
- 21. Wang L, Wang FS, Gershwin ME. Human autoimmune
diseases: a comprehensive update. Journal of Internal Medicine
2015; 278 (4): 369-95. doi: 10.1111/joim.12395
- 22. Hetta M, Moawad A, Hamed M, Ismail A. In vitro and in vivo
hypolipidemic activity of spinach roots and flowers. Iranian
Journal of Pharmaceutical Research 2017; 16 (4): 1509-
1519. doi: 10.22037/IJPR.2017.2113
- 23. Kim JH, Kismali G, Gupta SC. Natural products for the
prevention and treatment of chronic inflammatory diseases:
integrating traditional medicine into modern chronic diseases
care. Evidence-Based Complementary and Alternative
Medicine 2018; 2018: 9837863. doi: 10.1155/2018/9837863
- 24. Shahouzehi B, Sepehri G, Sadeghiyan S, Masoomi-Ardakani
Y. Effect of Pistacia atlantica resin oil on anti-oxidant,
hydroxyprolin and VEGF changes in experimentally-induced
skin burn in rat. World Journal of Plastic Surgery 2018; 7 (3):
357-363. doi: 10.29252/wjps.7.3.357
- 25. Ross AC. Vitamin A and retinoic acid in T cell related
immunity. American Journal of Clinical Nutrition 2012; 96 (5):
1166S-1172S. doi: 10.3945/ajcn.112.034637
- 26. Kobayashi K, Kaneda K, Kasama T. Immunopathogenesis
of delayed-type hypersensitivity. Microscopy Research and
Technique 2001; 53: 241-245. doi:10.1002/jemt.1090
- 27. Murdaca G, Colombo BM, Puppo F. The role of Th17
lymphocytes in the autoimmune and chronic inflammatory
diseases. Internal and Emergency Medicine 2011; 6 (6): 487-
495. doi: 10.1007/s11739-011-0517-7
- 28. Tigno-Aranjuez JT, Lehmann PV, Tary-Lehmann M.
Dissociated induction of cytotoxicity and DTH by CFA and
CpG. Journal of Immunotherapy 2009; 32 (4): 389-398. doi:
10.1097/CJI.0b013e31819d79a7
- 29. Saraiva M, O’Garra A. The regulation of IL-10 production by
immune cells. Nature Reviews Immunology 2010; 10 (3): 170-
181. doi: 10.1038/nri2711
- 30. Brockmann L, Soukou S, Steglich B. Molecular and functional
heterogeneity of IL-10-producing CD4+ T cells. Nature
Communications 2018; 9 (1): 5457. doi: 10.1038/s41467-018-
07581-4