Babu Lal JANGIR, Raj Bahadur BIND, Pawan KUMAR, Ramesh SOMVANSH

Pathological studies and detection of different bovine papilloma virus types in buffalo cutaneous warts

Bovine papillomatosis is a common viral disease of cattle but is relatively less common in buffaloes. Bovine papilloma virus(BPV) -1 and -2 are mostly associated with cutaneous warts in buffaloes. The aim of the present work was to study the pathomorphologyand association of different BPV types in buffalo cutaneous warts. A total of 10 cases were included in the present study, which revealedthat growths were either single or multiple, variable in shape and size, and present at different body sites. Histopathologically, thesewere categorized as papilloma, fibropapilloma, and fibroma. Polymerase chain reaction (PCR) revealed the presence of BPV-1, -2, and-5 DNA. Quantitative real-time PCR showed varying amounts of viral DNA copy numbers. On immunohistochemistry, nuclear Ki-67expression was observed mainly in the cells of suprabasal layers. In conclusion, DNA of BPV-1, -2, and -5 was detected either alone ortheir mixed infections. Nuclear Ki-67 immunoreactivity in the suprabasal layer indicated that this is the cellular proliferation site.

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