Mycoplasma arginini : high frequency involvement in goat pneumonia
Mycoplasmas are an important class of bacteria associated with caprine pneumonia. Some of the main species involved are M. mycoides subsp. capri, M. capricolum subsp. capricolum, and M. capricolum subsp. capripneumoniae. In addition, there are frequent reports of M. arginini involvement. In the present study an attempt was made to see the association of various mollicutes in caprine pneumonia. In total 244 pneumonic goat lung samples collected over a 10-year period (2003-2012) were screened for the isolation and identification of associated mollicutes. The identification was done on the basis of biochemical tests, growth inhibition test, PCR, and sequencing. The most frequent isolates were Acholeplasma (20/244, i.e. 8.19%) and Mycoplasma arginini (13/244, i.e. 5.3%), followed by M. mycoides subsp. capri (1/244) and M. agalactiae (1/244). Seven representative isolates of M. arginini were subjected to partial sequencing of the rpoB gene. On phylogenic analysis, M. arginini isolates were found to be highly identical, exhibiting >98% identity with that of the standard M. arginini (ATCC 23243) strain. The results encourage exploration of the role of M. arginini in goat pneumonia in much detail, due to its high frequency involvement.
___
- Akwuobu CA, Ayling RD, Chah KF, Oboegbulem SI. Studies
into the prevalence of
Mycoplasma
species in small ruminants
in Benue State, North-central Nigeria. Trop Anim Health Prod
2014; 46: 1087-1092.
- Nicholas RAJ. Improvements in the diagnosis and control of
disease of small ruminants caused by Mycoplasmas. Small
Rum Res 2002; 45: 145-149.
- Azizi S, Tajbakhsh E, Rezaii A, Nekouei SH, Namjoo AR. The
role of
Mycoplasma ovipneumoniae
and
Mycoplasma arginini
in
pneumonic lungs of slaughtered sheep. Revue Méd Vét 2011;
162: 310-315.
- Ayling RD, Nicholas RAJ. Diseases of sheep. In: Mycoplasma
Respiratory Infections. 4th ed. Oxford, UK: Blackwell
Publishing; 2007. pp. 231-235.
- Gonçalves R, Mariano I, Núñez A, Branco S, Fairfoul G,
Nicholas R. Atypical non-progressive pneumonia in goats. Vet
J 2010; 183: 219-221.
- Kumar A, Rahal A, Chakraborty S, Verma A K, Dhama K.
Mycoplasma agalactiae
, an etiological agent of contagious
agalactia in small ruminants: a review. Vet Med Int 2014;
http://dx.doi.org/10.1155/2014/286752.
- Elfaki MG, Abbas B, Mahmoud OM, Kleven SH. Isolation and
characterization of
Mycoplasma arginini
from camels (
Camelus
dromedarius
) with pneumonia. Comp Immunol Microbiol
Infect Dis 2002;
25: 49-57.
- Gupta S, Chahota R, Bhardwaj B, Malik P, Verma S, Sharma
M. Identification of Chlamydiae and Mycoplasma species in
ruminants with ocular infections. Lett Appl Microbiol 2015;
60: 135-139.
- Alley MR, Quinlan JR, Clarke JK. The prevalence of
Mycoplasma ovipneumoniae
and
Mycoplasma arginini
in the
respiratory tract of sheep. New Zeal Vet J 1975; 23: 137-141.
- Chima JC, Ojo MO, Molokwu JU, Okewole PA. Characterisation
of mycoplasmas isolated from genital tract infections of sheep
in Nigeria. Rev Sci Tech Off Int Epiz 1995; 14: 865-871.
- Niang M, Rosenbusch RF, Lopez-Virella J, Kaeberle ML.
Differential serologic response to
Mycoplasma ovipneumoniae
and
Mycoplasma arginini
in lambs affected with chronic
respiratory disease. J Vet Diagn Invest 1999; 11: 34-40.
- Prayson MJ, Venkatarayappa I, Srivastava M, Northern I,
Burdette SD. Deep infection with
Mycoplasma arginini
in an
open femur fracture secondary to an African lion bite: a case
report. Injury Extra 2008; 39: 243-246.
- Watanabe M, Hitomi S, Goto M, Hasegawa Y.
Bloodstream infection due to
Mycoplasma arginini
in an
immunocompromised patient. J Clin Microbiol 2012; 50:
3133-3135.
- Olaogun OM, Kanci A, Barber SR, Tivendale KA, Markham PF,
Marenda MS, Browning GF. Genetic diversity of
Mycoplasma
arginini
isolates based on multilocus sequence typing. Vet
Microbiol 2015; 180: 123-128.
- Gagea MI, Bateman KG, Shanahan RA, van Druemel T,
McEwen BJ, Carman S, Archambault M, Caswell JL. Naturally
occurring
Mycoplasma bovis
-associated pneumonia and
polyarthritis in feedlot calves. J Vet Diagn Invest 2006; 18: 29-
40.
- Chazel M, Tardy F, Grand DL, Calavas D, Poumarat F.
Mycoplasmoses of ruminants in France: recent data from the
national surveillance network. BMC Vet Res 2010; 6: 32-39.
- Erno H, Stipkovits L. Bovine mycoplasmas: cultural and
biochemical studies, II Acta Vet Scand 1937; 14: 450-463.
- Aloutto BB, Witter RG, Williams CO, Faber JE. Standardized
bacteriological techniques for the characterization of
mycoplasma species. Int J Syst Bacteriol 1970; 20: 35-38.
- Clyde WA. Mycoplasma species identification based upon
growth inhibition by specific antisera. J Immunol 1964; 92:
958-965.
- Sambrook J, Russel DW. Molecular Cloning. 3rd ed. New York,
NY, USA: Cold Spring Harbor Laboratory Press; 2001.
- van Kuppeveld FJM, Van Der Logt JTM, Angulo AF, Zoest MJV,
Quint WGV, Niesters HGM, Galama JMD, Melchers WJG.
Genus- and species-specific identification of mycoplasmas by
16S rRNA amplification. Appl Environ Microbiol 1992; 58:
2606-2615.
- Sillo P, Pinter D, Ostorhazi E, Mazan M, Wikonkal N, Ponyai
K, Volokhov DV, Chizhikov VE, Szathmary S, Stipkovits L et
al. Eosinophilic fasciitis associated with
Mycoplasma arginini
infection. J Clin Microbiol 2012; 50: 1113-1117.
- Hernandez L, Lopez J, St-Jacques M, Ontiveros L, Acosta
J, Handel K.
M. mycoides
subsp.
capri
associated with goat
respiratory disease and high flock mortality. Can Vet J 2006;
47: 366-369.
- Tola S, Angioi A, Rocchigiani AM, Idini G, Manunta D, Galleri
G, Leori, G. Detection of
Mycoplasma agalactiae
in sheep milk
samples by polymerase chain reaction. Vet Microbiol 1997; 54:
17-22.
- Saad NM, Hameed KGA. Detection of
Mycoplasma
species in
raw milk of lactating animals in Assiut and Qena city of Egypt.
Vet World 2012; 5: 80-85.
- Banerjee M, Singh N, Gupta PP. Isolation of mycoplasmas and
acholeplasmas from pneumonic lesions in sheep and goats in
India. Zentralbl Vet Med (B) 1979; 26: 689-695.
- Ammar AM, Eissa SI, El-Hamid MIA, Ahmed HA, ElAziz
AEE. Advanced study on mycoplasmas isolated from sheep.
Zag Vet J 2008; 36: 128-137.
- Goltz JP, Rosendal S, McCraw BM, Ruhnke HL. Experimental
studies on the pathogenicity of
Mycoplasma ovipneumoniae
and
Mycoplasma arginini
for the respiratory tract of goats. Can
J Vet Res 1986; 50: 59-67.