Murat KAPLAN, Gülnur KALAYCI, Kemal PEKMEZ, Abdurrahman Anıl ÇAĞIRGAN, Buket ÖZKAN

Molecular detection and phylogenetic analysis of the honeybee (Apis mellifera) sacbrood virus in Turkey

Sacbrood virus (SBV) is widely distributed in honeybees. It is an infectious and contagious disease, which affects both thelarvae and adult stages of honeybees (Apis mellifera). This study reports the detection of sacbrood virus in Turkey in 2017. Phylogeneticanalysis based on partial sequences encoding the polyprotein gene was carried out in SBV-infected bees from four different apiaries.Phylogenetic analysis revealed noteworthy results. Although European-South American, Asian, and Korean genotypes have a maximumof 5.3% variance with each other, the variation rates between them and the Turkish genotype were 10.4%, 11.4%, and 12.5%, respectively.Sequences of Turkish isolates did not fit into the previously reported genotype groups; furthermore, they created a completely separatebranch according to phylogeny. This new branch was therefore named the Turkish genotype.

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1. Ball BV, Bailey L. Viruses. In: Morse RA, Flottum K, editors. Honey Bee Pests, Predators, & Diseases. Medina, OH, USA: The A. I. Root Company; 1997. pp. 11-31.
2. Bailey L. Recent research on honey bee viruses. Bee World 1975; 56: 55-64.
3. Chen YP, Siede R. Honey bee viruses. Adv Vir Research 2007; 70: 33-80.
4. Tentcheva D, Gauthier L, Zappulla N, Dainat B, Cousserans F, Colin ME, Bergoin M. Prevalence and seasonal variations of six bee viruses in Apis mellifera L. and Varroa destructor mite populations in France. Appl Environ Microbiol 2004; 70: 7185-7191.
5. Berenyi O, Bakonyi T, Derakhshifar I, Kögkberger H, Nowotny N. Occurrence of six honeybee viruses in diseased Austrian apiarie. Appl Environ Microbiol 2006; 72: 2414-2420.
6. Chen YP, Pettis JS, Collins A, Feldlaufer MF. Prevalence and transmission of honeybee viruses. Appl Environ Microbiol 2006; 72: 606-611.
7. Bailey L. Honey bee pathology. Annu Rev Entomol 1968; 13: 191-212.
8. Ghosh RC, Ball BV, Wilcocks MM, Carter MJ. The nucleotide sequence of sacbrood virus of the honey bee: an insect picornalike virus. J Gen Virol 1999; 80: 1541-1549.
9. Sguazza GH, Reynaldi FJ, Galosi CM, Pecoraro MR. Simultaneous detection of honey bee viruses by multiplex PCR. J Virol Methods 2013; 194: 102-106.
10. Altschul SF, Gish W, Miller W, Myers EW, Lipman DJ. Basic local alignment search tool. J Mol Biol 1990; 215: 403-410.
11. Tamura K, Stecher G, Peterson D, Filipski A, Kumar S. MEGA6: Molecular evolutionary genetics analysis version 6.0. Mol Biol Evol 2013; 30: 2725-2729.
12. Jones DT, Taylor WR, Thornton JM. The rapid generation of mutation data matrices from protein sequences. Comput Appl Biosci 1992; 8: 275-282.
13. Blanchard P, Guillot S, Antunez K, Köglberger H, Kryger P, Miranda JR, Franco S, Chauzat MP, Thiery R, Ribiere M. Development and validation of a real-time two-step RT-qPCR TaqMan assay for quantitation of sacbrood virus (SBV) and its application to a field survey of symptomatic honey bee colonies. J Vir Methods 2014; 197: 7-13.
14. White GF. Sacbrood. US Department of Agriculture Bulletin No: 431. Washington, DC, USA: Department of Agriculture; 1917.
15. Grabensteiner E, Ritter W, Carter MJ, Davison S, Pechhacker H, Kolodziejek J, Boecking O, Derakhshifar I, Moosbeckhofer R, Licek E et al. Sacbrood virus of the honeybee (Apis mellifera): rapid identification and phylogenetic analysis using Reverse Transcription PCR. Clin Diagn Lab İmmunol 2001; 8: 93-104.
16. Antunez K, D’Alessandro B, Corbella E, Ramallo G, Zunino P. Honeybee viruses in Uruguay. J Invertebr Pathol 2006; 93: 67-70.
17. Allen MF, Ball BV. The incidence and world distribution of honey bee viruses. Bee World 1996; 77: 141-162.
18. Kukielka D, Sanchez-Vizcaino JM. One-step real-time quantitative PCR assays for the detection and field study of sacbrood honeybee and acute bee paralysis viruses. J Virol Methods 2009; 161: 240-246.
19. Choe SE, Nguyen TT, Hyun BH, Noh JH, Lee HS, Lee CH, Kang SW. Genetic and phylogenetic analysis of South Korean sacbrood virus isolates from infected honey bees (Apis cerena). Vet Microbiol 2012; 157: 32-40.