Mis ahtapot, Eledone moschata (Lamarck 1799), (Cephalopoda: Octopodidae) için 2-fenoksietanolün anestezik olarak etkisi

Mis ahtapot Eledone moschata (Lamarck, 1799) için 2-fenoksietanol (2-PhOH)’ün anestetik olarak etkisi incelendi. 2-PhOH’ün farklı dozları (1.0, 1.2, 1.4, 1.6 ve 1,8 mL L–1) 20 litrelik şeffaf kabın içinde bulunan ve sürekli havalandırılan 10 litre deniz suyunda (15,3 °C sıcaklıkta, tuzluluk ‰ 37,2, pH 8,2 ve O2 8 mg L–1) hazırlandı. Her denemede altı ahtapot kullanıldı. Her hayvan için anestezi zamanı, ki vantuzların yapışma kabiliyetinin kaybolması (Safha A3) olarak tanımlandı, belirlendikten sonra ahtapotlar vakit geçirilmeden plastik ayıltma tanklarına(içinde 430 L iyi havalandırılmış deniz suyu bulunan) nakledildi, ayılma normal nefes alış verişi (Safha R4; 12-13 nefes dak–1) olarak tanımlandı, burada 48 saat boyunca ölüm olup olmayacağı gözlendi. Kullanılan 2-PhOH konsantrasyonlar içinde bayılma ve ayılma zamanlarında istatistiksel olarak önemli farklar vardı (ANOVA, P < 0,05). Mis ahtapot, 1,2, 1,4 ve 1,6 mL L–1 2-PhOH konsantrasyonlarında etkili şekilde anestezi edildi (P > 0,05) ve 48 saat içinde ölüm gözlenmedi. Ancak, anesteziden 24 saat sonra 1.0 mL L–1 2-PhOH konsantrasyonunda % 50 ölüm ve 1,8 mL L–1 2-PhOH konsantrasyonunda 48 saat içinde % 100 ölüm oldu. Ayılma zamanına bağlı olarak 1,6 mL L–1 2-PhOH konsantrasyonu mis ahtapotun anestezisi için en etkili doz olarak görüldü

Anahtar Kelimeler:

Anestetik, 2-fenoksietanol, Eledone moschata, mis ahtapot

Efficacy of 2-phenoxyethanol as an anaesthetic for the musky octopus, Eledone moschata (Lamarck 1799), (Cephalopoda: Octopodidae)

The efficacy of 2-phenoxyethanol (2-PhOH) as an anaesthetic for the musky octopus, Eledone moschata (Lamarck, 1799), was examined. Different doses (1.0, 1.2, 1.4, 1.6, and 1.8 mL L-1) of 2-PhOH were prepared in 20 L transparent plastic containers containing 10 L of continually aerated seawater (pH 8.2, O2 8 mg L-1, salinity 37.2 per thousand at 15.3 °C). Six individuals were used in each experiment. After quantifying the anaesthetic time for each animal, which was defined as the loss of sucking intensity (Stage A3), the specimens were transferred to a plastic recovery tank (430 L of aerated seawater), defined as regular breathing (Stage R4; 12-13 breaths min-1), where they were observed for 48 h to document any mortality. There were significant differences in induction and recovery time among the various 2-PhOH concentrations (ANOVA, P < 0.05). The musky octopus was anaesthetised effectively at 1.2, 1.4, and 1.6 mL L-1 of 2-PhOH (P > 0.05) and no mortality was observed within 48 h. However, 50% mortality occurred at 1 mL L-12-PhOH within 24 h after anaesthesia, and 100% mortality at 1.8 mL L-1 of 2-PhOH within 48 h. Based on the recovery time, a dose of 1.6 mL L-1 of 2-PhOH was considered the most effective dose for anaesthetising the musky octopus.

Keywords:

2-phenoxyethanol, Eledone moschata, musky octopus, Anaesthetic,

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