Mikroenjeksiyon uygulanmış hibrid fare zigotlarının outbred ve hibrid alıcılara transferleri sonrasında elde edilen doğum oranlarının karşılaştırılması
The purpose of this study was to compare the birth rates after transfers of microinjected hybrid mouse zygotes into hybrid CB6F1 (C57BL/6J X BALB/c) and outbred CD-1 foster mothers. One or two hours after microinjection of a b -actin-Gfp gene construct into the male pronucleus, hybrid mouse zygotes were transferred into CB6F1 and CD-1 foster mothers. In addition, non-microinjected hybrid zygotes were transferred into foster mothers of both strains. The overall data demonstrate that outbred CD-1 foster mothers are a better choice for transfer of microinjected hybrid mouse embryos, and there was a statistical difference between the transfers of microinjected zygotes into CB6F1 and CD-1 foster mothers (P
Comparing the birth rates of microinjected hybrid mouse zygotes transferred into outbred and hybrit foster mothers
The purpose of this study was to compare the birth rates after transfers of microinjected hybrid mouse zygotes into hybrid CB6F1 (C57BL/6J X BALB/c) and outbred CD-1 foster mothers. One or two hours after microinjection of a b -actin-Gfp gene construct into the male pronucleus, hybrid mouse zygotes were transferred into CB6F1 and CD-1 foster mothers. In addition, non-microinjected hybrid zygotes were transferred into foster mothers of both strains. The overall data demonstrate that outbred CD-1 foster mothers are a better choice for transfer of microinjected hybrid mouse embryos, and there was a statistical difference between the transfers of microinjected zygotes into CB6F1 and CD-1 foster mothers (P
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- 1. Bagis, H., Papuccuo¤lu, S.: Studies on the production of transgenic mice. Tr. J. of Veterinary and Animal Sciences, 1997; 21: 287-292
- 2. Hogan, B, Beddington, R., Costantini, F., and Lacy, E.: Manipulating the Mouse Embryo (2nd edn.) Cold Spring Harbor Laboratory Press. Cold Spring Harbor, 1994; pp.217-252.
- 3. Brinster, RL., Chen, HY., Trumbauer, ME., Yagle, MK., and Palmiter, RD.: Factors affecting the efficiency of introducing foreign DNA into mice by microinjecting eggs. Proc. Natl. Acad. Sci., 1985; 82: 4438-4442.
- 4. DePamphilis, ML., Herman, SA., Enez-Salas, SA., Chalifour, LE., Wirak, DO., Cupo, DY., and Mrancla, M.: Microinjecting DNA into mouse ova to study DNA replication and gene expression and to protect transgenic animals. BioTechniques., 1988; 6: 662.
- 5. Lin, T.P.: Microinjection of mouse eggs. Science, 1966; 151, 333-337.
- 6. Bagis, H., Keskintepe, L.: Application of green fluorescent protein as a marker for selection of transgenic mouse embryos before implantation. Tr.J. of Biology., 2001; 25 (2), 123-131.
- 7. Rzucidlo, S.J., Bagis, H., McGraw, R.A., and Brackett, B.G.: Production and identification of transgenic mice for cytomegalovirus-controlled expression of glutathione synthetase. Southeastern Chapter of the Society of Toxicology Annual Meeting (SESOT). October 2-3, 1997, USA
- 8. Chatot, C.L., Ziomek, C.A., Bavister, B.D., Lewis, J.L., Torres, I.: An improved culture medium supports development of randombred 1-cell mouse embryos in vitro. J. Reprod. Fert., 1989; 86: 679-688.
- 9. Bagis, H., Keskintepe, L., Odaman H., Sagirkaya, H.: Effect of CZB medium on the two cell block of preimplantation mouse embryos. Tr.J. of Veterinary & Animal Sciences. 2001; 25 (5).
- 10. Bagis, H.: Studies on the production of transgenic mice. Ph.D thesis. Faculty of Veterinary Medicine. University of İstanbul, 1994.
- 11. Arat, S., Bagis, H, Cirakoglu, B.: Microinjection into one-cell mouse embryos. Tr.J. of Veterinary & Animal Sciences, 1994; 18, 237-240.
- 12. Arat, S., Bagis, H, Cirakoglu, B.: Studies on the transfer of single cell embryos to oviduct in mouse strains. Tr.J. of Veterinary & Animal Sciences, 1994; 18, 169-173.
- 13. Gordon, J.W., Scangos, G.A., Plotkin, D.J., Barbosa, J.A., and Rudle, R.H.: Genetic transformation of mouse embryos by microinjection of purified DNA. Proc.Natl.Acad.Sci., 1980; 77, 7380-7384.
- 14. Palmiter, R.D., Norstedt, G., gelinos, R.E., Brinster, R.L.: Metallothionenin–human GH fusion genes stimulate growth of mice. Science, 1983; 222, 809-814.
- 15. Dincturk, B., Turkmenler, A., Arat S., Bagis, H., Cirakoglu, B.: A transgenic mouse model with human growth hormone. Tr.J. of Biology, 1996; 20, 303-311.
- 16. Lovell-Badge, R.H.: Transgenic animals, New Advances in the field. Nature, 1985; 315, 628-629.
- 17. Bucchini, D., Ripoche, M.A., Stinnakre, M.G., Desbois, P., Lores, P., Monthioux, E., Absil, J., Lepesant, J.A., Picter, R., Jami, J.: Pancreatic expression of human insulin gene in transgenic mice. Proc.Natl.Acad.Sci., 1986; 83, 2511-2515.
- 18. Costantini, F., Lacy, E.: Introduction of a rabbit b-globin gene into the mouse germ line. Nature, 1981; 294, 92-94.