Nurhayat GÜLMEZ, Şahin ASLAN, Hakan KOCAMIŞ, Mümtaz NAZLI

Immunohistochemical distribution of cells containin insulin, glucagon and somatostatin in the goose (Anser anser) pancreas

Bu çalışmanın amacı, insulin (B), glukagon (A) ve somatostatin (D) içeren hücrelerin kaz pankreasındaki dağılımını saptamaktı. Küçük doku parçalan anestezi altındaki beş erişkin kaz pankreasından alındı. Kesitler, genel gözlem için Crossman'ın bağ doku boyasıyla, pankreas adacıkları için ise Gomori'nin boyama metodu ile boyandı. A, B, ve D hücrelerinin dağılımı için, Avidin-Biotin-Kompleks metodu kullanıldı. A hücrelerinin çoğunlukta olduğu A adacıklarında B hücrelerine rastlanmadı. B adacıklarında hücrelerin çoğu kapillarların etrafında daire tarzında yerleşmiş B hücreleriydi, bu adacıklarda periferal yerleşimli A hücrelerine de rastlandı. Insulin granülleri B hücrelerinin damar kutbuna yerleşmişti. D hücreleri A adacıklarının her tarafına dağılmış olmalarına rağmen, B adacıklarında genellikle periferal olarak yerleşmişti. Pankreas dokusunda genel olarak D hücreleri, A ve B hücrelerine göre daha yaygındı. Kazların pankreasında insulin, glukagon, ve somatostatin içeren hücrelerin dağılımının, küçük farklarla birlikte, diğer kanatlılarınki ile benzerlik gösterdiği saptanmıştır.

Insulin, glukagon ve somatostatin içeren hücrelerin kaz (Anser anser) pankreasında immunohistokimyasal dağılımı

Abstract: The purpose of the present study was to determine the distribution of cells containing glucagon (A), insulin (B), and somatostatin (D) in the goose pancreas. Small pieces of tissue from 5 adult geese (Anser anser) pancreases were dissected under deep ether anaesthesia. Sections were stained with Crossmon's connective tissue stain for general observations and Gomori's method for pancreatic islet cells. Sections were further processed for standard immunohistochemical techniques using the avidin-biotin-complex method for the distribution of A, B and D cells. There were no B cells inside the A islets, which were mainly composed of A cells. B islets were mainly composed of insulin-producing B cells arranged in a circular fashion around blood capillaries. Granules containing insulin were localised in blood vessel sites of the B cells. Although D cells were distributed in almost all sites of the A islets, they were mainly localised at the periphery of the B islets. D cells throughout the pancreas were more common than that of B and A cells. The distribution of cells containing insulin, glucagon and somatostatin in the goose pancreas, with small differences, was similar to that of other avian species.

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