Sami Mehmet SERİN, Seda TEZCAN, Gönül ASLAN, Selim ÖZER, Sevim Pınar BULDUKLU

Genetic heterogeneity and antibiotic susceptibility of Vibro alginolyticus strains isolated from horse-mackerel (Trachurus trachurus L., 1758)

Bu çalışma istavrit cinsi (Trachurus trachurus L, 1758) balıklarda halofılik Vibrio tülerinin varlığının araştırılması amacıyla gerçekleştirilmiştir. Mersin ilindeki balık pazarları ve süpermarketlerden 04.04.2006 ve 16.05.2006 tarihleri arasında toplam 80 balık örneği toplanmıştır. Bütün balık örnekleri halofılik Vibrio türleri yönünden mikrobiyolojik olarak incelenmiştir. Dokuz adet (% 11,25) Vibrio türü izole edilmiştir ve bunların hepsi V. alginolyticus olarak identifiye edilmiştir. V. alginolyticus suşlarımn moleküler epidemiyolojik ayrımı arbitrarily primed-polimeraz zincir reaksiyonu (AP-PZR) ve antibiyotik duyarlılıkları Kirby-Bauer disk difüzyon metotları ile incelenmiştir. AP-PZR ile genotipik heterojenite ayrımı gerçekleştirilen bu 9 izolatın, 3 tanesinin aynı genotipik kaynaktan, diğer 6 izolatın da farklı kaynaktan köken aldığı ortaya~ çıkarılmıştır. İzolatların tümü ampisilin, sefalotin ve streptomisine dirençli ve levofloksasin, ofloksasin, sefotaksim, nalidiksik asit, tetrasiklin, piperasilin-tazobaktam, kloramfenikol, imipenem ve aztreonam'a karşı duyarlı olarak bulunmuştur. İzolatlardan biri cefepime'e, biri nitrofurantoin ve biri de siprofloksasin'e dirençli bulunmuştur. Dokuz izolattan birinin eritromisin, birinin novobiosin ve üçünün sulbactam-sefaperozon'a duyarlı olduğu tespit edilmiştir.

İstavrit (Trachurus trachurus L., 1758) balığından izole edilen Vibrio alginolyticus suşlarının genetik heterojenitesi ve antibiyotik hassasiyeti

This study was performed to investigate the presence of halophylic Vibrio spp. in horse-mackerel (Trachurus trachurus L., 1758). In total, 80 fish samples were collected from fish markets and supermarkets between 04.04.2006 and 16.05.2006 in the city of Mersin. All fish samples were microbiologically tested for halophylic Vibrio spp. Nine (11.25%) Vibrio spp. strains were isolated and identified as V. alginolyticus. Molecular epidemiological discrimination and antimicrobial susceptibility patterns of V. alginolyticus strains were also analysed by arbitrarily primed polymerase chain reaction (AP-PCR) and Kirby-Bauer disc diffusion, respectively. Genotypic heterogeneity of these 9 isolates discriminated by AP-PCR determined that while only 3 isolates were from the same genotypic source the other 6 isolates were from different sources. All isolates were resistant to ampicillin, cephalothin, and streptomycin, and susceptible to levofloxacin, ofloxacin, cefotaxime, nalidixic acid, tetracycline, piperacillin-tazobactam, chloramphenicol, imipenem, and aztreonam. One isolate was resistant to cefepime, 1 to nitrofurantoin, and 1 to ciprofloxacin. One of the 9 isolates was susceptible to erythromycin and 1 to novobiocin, and 3 isolates were susceptible to sulbactam-cefoperazone.

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