Runshen JIANG, Xingyong CHEN, Ruhua WEI, Zhaoyu GENG

Expression of plasma prolactin and pituitary prolactin mRNA around the broody cycle in Wan-xi White goose

A systematic evaluation of the dynamic changes of prolactin (PRL) around the whole cycle of broodiness in domestic fowl has not been performed. We started to investigate the overall changing profile of PRL expression at both protein and mRNA levels from pre- to post-broodiness in Wan-xi White goose, an indigenous breed in East China. The onset of broodiness was recorded, and the plasma PRL concentration and the pituitary PRL mRNA level at 5 stages were measured, in that order, around the first broody cycle. The expression level of pituitary PRL mRNA was highly correlated with the plasma PRL level (r = 0.92), and both changed synchronically around the broody cycle. They significantly increased at the onset of broodiness, but rapidly dropped during the early half period of broodiness, until the later half period of broodiness, and post-broodiness as well, where they reached a similar level as that of pre-broodiness (the laying period). Therefore, intervention on PRL could be a useful tool in regulating goose broodiness, as well as potentially for other domestic fowls.

Anahtar Kelimeler:

Key words: Prolactin, mRNA, broody cycle, Wan-xi White Goose

Expression of plasma prolactin and pituitary prolactin mRNA around the broody cycle in Wan-xi White goose

Keywords:

Key words: Prolactin, mRNA, broody cycle, Wan-xi White Goose,

PDF

___

Sockman, K.W., Schwabl, H., Sharp, P.J.: Th e role of prolactin in the regulation of clutch size and onset of incubation behavior in the American kestrel. Horm. Behav., 2000; 38: 168-76.
Reddy, I.J., David, C.G., Sarma, P.V., Singh, K.: Th e possible role of prolactin in laying performance and steroid hormone secretion in domestic hen (Gallus domesticus). Gen. Comp. Endocrinol., 2002; 127: 249-55.
Burke, W.H., Dennison, P.T.: Prolactin and luteinizing hormone levels in female turkeys (Meleagris gallopavo) during a photoinduced reproductive cycle and broodiness. Gen. Comp. Endocrinol., 1980; 41: 92-100.
Bacon W.L., Burke, W.H., Nestor, K.E., Brown, K.I.: Infl uence of genetic increases in egg production on traits associated with broodiness in turkeys. Poult. Sci., 1983; 62: 2460-73.
Talbot, R.T., Hanks, M.C., Sterling, R.J., Sang, H.M., Sharp, P.J.: Pituitary prolactin messenger ribonucleic acid levels in incubating and laying hens: eff ects of manipulating plasma levels of vasoactive intestinal polypeptide. Endocrinology, 1991; 129: 496-502.
Karatzas, C.N., Guemene, D., Zadworny, D., Kuhnlein, U.: Changes in expression of the prolactin and growth hormone gene during diff erent reproductive stages in the pituitary gland of turkeys. Reprod. Nutr. Dev., 1997; 37: 69-79.
Hall, M.R.: External stimuli aff ecting incubation behavior and prolactin secretion in the duck (Anas platyrhynchos). Horm. Behav., 1987; 21: 269-287.
Hall, M.R.: Endocrinological and behavioral changes associated with the onset of incubation in the duck. Physiol. Behav., 1991; 50: 311-6.
Yen, C.F., Lin, H.W., Hsu, J.C., Lin, C., Shen, T.F., Ding, S.T.: Th e expression of pituitary gland genes in laying geese. Poult. Sci., 2006; 85: 2265-69.
SAS Institute: SAS User’s Guide: Statistics. SAS Institute, Inc., Cary, NC, 2001.
Lea, R., Dods, A., Sharp, P., Chadwick, A.: Th e possible role of prolactin in the regulation of nesting behavior and the secretion of luteinizing hormone in broody bantams. J. Endocrinol., 1981; 91: 89-97.
March, J.B., Sharp, P.J., Wilson, P.W., Sang, H.M.: Eff ect of active immunization against recombinant-derived chicken prolactin fusion protein on the onset of broodiness and photoinduced egg laying in bantam hens. J. Reprod. Fertil., 1994; 101: 227-33.
Crisostomo, S., Guemene, D., Garr eau-Mills, M., Morvan, C., Zadworny, D.: Prevention of incubation behavior expression in turkey hens by active immunization against prolactin. Th eriogenology, 1998; 50: 675-90.
Crisostomo, S., Guemene, D., Garreau-Mills, M., Zadworny, D.: Prevention of the expression of incubation behavior using passive immunization against prolactin in turkey hens (Meleagris gallopavo). Reprod. Nutr. Dev., 1997; 37: 253-66.
Sharp, P.J., Scanes, C.G., Williams, J.B., Harvey, S., Chadwick, A.: Variations in concentration of prolactin, luteinizing hormone, growth hormone and progesterone in the plasma of broody bantams (Gallus domesticus). J. Endocrinol., 1979; 80: 51-7.
Goldsmith, A.R., Hall, M.: Prolactin concentrations in the pituitary gland and plasma of Japanese quail in relation to photoperiodically induced sexual maturation and egg laying. Gen. Comp. Endocrinol., 1980; 42: 449-54.
Bluhm, C.K., Phillips, R.E., Burke, W.H.: Serum levels of luteinizing hormone (LH), prolactin, estradiol, and progesterone in laying and nonlaying canvasback ducks (Aythya valisineria). Gen. Comp. Endocrinol., 1983; 52: 1-16.
Wong, E.A., Ferrin, N.H., Silsby, J.L.: Cloning of a turkey prolactin cDNA: Expression of prolactin mRNA throughout the reproductive cycle of the domestic turkey (Meleagris gallopavo). Gen. Comp. Endocrinol., 1991; 83: 18-26.
Kansaku, N., Ohkubo, T., Okabayashi, H., Guémené, D., Kuhnlein, U., Zadworny, D., Shimada, K.: Cloning of duck PRL cDNA and genomic DNA. Gen. Comp. Endocrinol., 2005; 141: 39-47.