Evaluation of the corpus leteum size throughout the cycle by ultrasonography and progesterone assay in cows

Bu çalışmanın amacı, ineklerde östrus siklusu boyunca corpus luteum (CL) (kaviteli veya kavitesiz) boyutları ile progesteron düzeyleri arasındaki ilişkinin saptanmasıdır. Postpartum ilk östrusunu göstermiş 10 Holstein inekte ultrasonografi kullanılarak 24 saat aralıklarla CL görüntüleri alındı. Progesteron düzeyi radioimmunoassay (RIA) tekniği ile belirlendi, ineklerin 1 tanesinde kısa (13 gün), 7 tanesinde normal (ortalama 22 gün) ve 2 tanesinde uzun (30 gün) siklus görüldü. Ölçümler bu siklus uzunluklarına göre değerlendirildi. Corpus luteum boyutları, ovulasyondan sonraki 2. günden itibaren ölçüldü. Corpus luteum maksimum boyutlara kısa siklusta (KS) 6. günde, normal siklusta (NS) ortalama 9. günde, uzun siklusta (US) 10. günde ulaştı. Progesteron düzeyi KS'de 0-4, NS ve US'de 0-2 günler arası 1 ng/ml'nin altındayken, KS'de 8. günde (7,31 ng/ml), NS'de 10. günde (9,81 ng/ml), US'de 18. günde (6,91 ng/ml) maksimum düzeye ulaştı. Corpus luteum boyutlarıyla P4 değeri arasındaki korelasyon KS'de 0,72 NS'de 0,92 ve US'de 0,54 olarak . saptandı. Sonuç olarak ineklerde CL boyutları ile P4 arasında korelasyon saptandı. Corpus luteum çapı ile alan, hacim ve çevre arasındaki korelasyon önemli bulundu.

İneklerde siklus boyunca corpus leteum boyutunun ultrasonografi ve progesteron ölçümü ile izlenmesi

The purpose of this study was to assess the correlation between the sizes of the corpus luteum (CL) with or without a cavity throughout the estrous cycle and progesterone (P4) levels in cows. Images of the CL were obtained by ultrasonography at intervals of 24 h in 10 Holstein cows after their first postpartum estrus. Progesterone levels were measured by râdioimmunassay (RIA). The duration of the estrous cycle was determined to be short (13 days), normal (approximately 22 days) and long (30 days) in 1, 7, and 2 cows, respectively. Measurements were evaluated based on the established cycle durations. The size of the CL was measured from the beginning of the second day after ovulation. The CL reached its maximum size on days 6, 9, and 10 in short (SC), normal (NC) and long (LC) cycles, respectively. The P4 levels were below 1 ng/ml on days 0-4 in SC, and 0-2 in NC and LC, and reached a maximum on days 8 (7.31 ng/ml), 10 (9.81 ng/ml) and 18 (6.91 ng/ml) in SC, NC and LC, respectively. The correlation between the size of the CL and the level of P4 was r: 0.72, 0.92, and 0.54 in cows indicating SC, NC, and LC, respectively. In conclusion, CL size and P4 level in cows were correlated. The correlation between the diameter, area, volume and perimeter of the CL was significant.

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