Effect of Effective Microorganisms on intestinal morphology and morphometry in Japanese quails
The aim of this study was to determine the effect of supplementation with Effective Microorganisms (EM) on the morphology
and the morphometry of the intestine, and on proliferation activity of crypt cells in Japanese quails. The EM group (16 birds) received
EM for 30 days in water (3 L/1000 L of water) and in feed (5 kg/1000 kg of feed) mixed with standard diet. The second group (16
birds) was established as the control group (no EM supplementation). Histopathological analysis revealed infiltration of lymphoid
cells mainly in the duodenum and jejunum similarly in the EM and the control groups. The EM group showed irregular villus surfaces
when compared to the control group. Morphometric analysis revealed significant differences in the EM group for villus width in the
duodenum (186.0 μm to 170.7 μm; P ≤ 0.05), in crypt depth (129.2 ?m to 110.7 μm; P ≤ 0.05), muscular layer thickness in the jejunum
(70.88 ?m to 57.44 ?m) (P ≤ 0.05), and muscular layer thickness in the ileum (76.88 ?m to 64.76 ?m;
P ≤ 0.05). Immunohistochemical
analysis of proliferating cell nuclear antigen activity was similar for the EM and the control groups: in the duodenum 90.82% in the
control group and 91.89% in the EM group, in the jejunum 93.32% in the control group and 94.53% in the EM group, and in the ileum
92.53% in the control group and 93.92% in the EM group. Supplementation with EM resulted in improved structure and functions of
the villi and changed their surface, height, width, and crypt depth.
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- Laudadio V, Passantino L, Perillo A, Lopresti G, Passantino A,
Khan RU, Tufarelli V. Productive performance and histological
features of intestinal mucosa of broiler chickens fed different
dietary protein levels. Poult Sci 2012; 91: 265-270.
- Starck JM, Rahmaan GHA. Phenotypic flexibility of structure
and function of the digestive system of Japanese quails. J Exp
Biol 2003; 206: 1887-1897.
- Murakami AE, Sakamoto MI, Natali MRM, Souza LMG,
Franco JRG. Supplementation of glutamine and vitamin E on
the morphometry of the intestinal mucosa in broiler chickens.
Poult Sci 2007; 86: 488-495.
- Smith MW, Mitchell MA, Peacock MA. Effects of genetic
selection on growth rate and intestinal structure in domestic
fowl (
Gallus domesticus
). Comp Biochem Physiol A Mol Integr
Physiol 1990; 97: 57-63.
- Timmerman HM, Veldman A, van den Elsen E, Rombouts
FM, Beynen AC. Mortality and growth performance of broilers
given drinking water supplemented with chicken-specific
probiotics. Poult Sci 2006; 85: 1383-1388.
- Kalavathy R, Abdullah N, Jalaludin S, Ho YW. Effects of
Lactobacillus
cultures on growth performance, abdominal
fat deposition, serum lipids and weight of organs of broiler
chickens. Br Poult Sci 2003; 44: 139-144.
- Khambualai O, Yamauchi K, Ruttanavut J, Incharoen T,
Kashimura J. Effect of sugar cane extract, commercial probiotic
and their mixture on growth performance and intestinal
histology in broiler chickens. Am J Anim Vet Sci 2010; 5: 132-
138.
- Pelicano ERL, Souza PA, Souza HBA,
Figueiredo DF, Boiago
MM, Carvalho SR, Bordon VF. Intestinal mucosa development
in broiler chickens fed natural growth promoters. Braz J
Poultry Sci 2005; 7: 221-229.
- Hassanpour H, Zamani Moghaddam AK, Khosravi M, Mayahi
M. Effects of synbiotic on the intestinal morphology and
humoral immune response in broiler chickens. Livest Sci 2013;
153: 116-122.
- Awad W, Ghareeb K, Bohn J. Intestinal structure and function
of broiler chickens on diets supplemented with a synbiotic
containing
Enterococcus faecium
and oligosaccharides. Int J
Mol Sci 2008; 9: 2205-2216.
- Gao J, Zhang HJ, Yu SH, Wu SG, Yoon I, Quigley J, Gao YP, Qi
GH. Effects of yeast culture in broiler diets on performance and
immunomodulatory functions. Poult Sci 2008; 87: 1377-1384.
- Santin E, Maiorka A, Macari M, Grecco M, Sanchez JC,
Okada TM, Myasaka AM. Performance and intestinal
mucosa development of broiler chickens fed diets containing
Saccharomyces cerevisiae
cell wall. J Appl Poult Res 2001; 10:
236-244.
- Zhao Q. Effect of EM on peanut production and soil fertility in
the red soil region of China. In: Proceedings of the Conference
on Effective Microorganisms for a Sustainable Agriculture and
Environment. France; 1995. pp. 99-102.
- Xu HL. Effects of a microbial inoculant and organic fertilizers
on the growth, photosynthesis and yield of sweet corn. J Crop
Prod 2001; 3: 183-214.
- Yamada K, Xu HL. Properties and applications of an organic
fertilizer inoculated with Effective Microorganisms. J Crop
Prod 2001; 3: 255-268.
- Yan PS, Xu HL. Influence of EM Bokashi on nodulation,
physiological characters and yield of peanut in nature farming
fields. J Sustain Agr 2002; 19: 105-112.
- Simeamelak M, Solomon D, Taye T. The effect of effective
microorganisms on production and quality performance of
Rhode Island Red layers. International Journal of Livestock
Production 2013; 4: 22-29.
- Gnanadesigan M, Sandhanasamy I, Ponnusamy S,
Lakshmanan R, Natarajan M, Rajagopal R. Quality evaluation
of egg composition and productivity of layers in EM (Effective
Microorganisms) treatments: a field report. Egyptian Journal
of Basic and Applied Sciences 2014; 1: 161-166.
- Jwher DMT, Abd SK, Mohammad AG. The study of using
effective microorganisms (EM) on health and performance of
broiler chicks. Iraqi Journal of Veterinary Sciences 2013; 27:
73-78.
- Wondmeneh E, Getachew T, Dessie T. Effect of effective
microorganisms (EM®) on the growth parameters of Fayoumi
and Horro chicken. International Journal of Poultry Science
2011; 10: 185-188.
- Wondmeneh E, Melesse A, Dessie T. Effect of effective
microorganisms on growth parameters and serum cholesterol
levels in broilers. Afr J Agric Res 2011; 6: 3841-3846.
- Sokół R, Michalczyk M, Spodniewska A, Barski D. The
influence of administering “effective microorganisms” to
pullets on chosen haematological and biochemical blood
indexes. Pol J Vet Sci 2009; 12: 519-522.
- Gesek M, Paździor K, Otrocka-Domagała I, Rotkiewicz T,
Szarek J. Fibromuscular dysplasia in arteries and in a vein in
broiler chickens. Pol J Vet Sci 2013; 16: 93-99.
- Braga III IS, Tanaka S, Itakura C, Mizutani M. Fibromuscular
dysplasia in intramuscular arteries of Japanese Quail (
Coturnix
coturnix japonica
). J Comp Pathol 1996; 114: 123-130.
- Gesek M, Sokół R, Welenc J, Tylicka Z, Korzeniowska
P, Kozłowska A, Wiśniewska AM, Otrocka-Domagała I.
Histopathological observations of the internal organs during
toltrazuril (Baycox®) treatment against naturally occurring
coccidiosis in Japanese Quail. Pak Vet J 2015; 35: 479-483.
- Tsirtsikos P, Fegeros K, Balaskas C, Kominakis A, Mountzouris
KC. Dietary probiotic inclusion level modulates intestinal
mucin composition and mucosal morphology in broilers. Poult
Sci 2012; 91: 1860-1868.
- Samli HE, Senkoylu N, Koc F, Kanter M, Agma A. Effects of
Enterococcus faecium
and dried whey on broiler performance,
gut histomorphology and intestinal microbiota. Arch Anim
Nutr 2007; 61: 42-49.