Determination of in situ degradation kinetics of some legume waste not used for human consumption

The objective of this experiment was to evaluate the chemical composition, in situ ruminal degradation kinetics, and proteinfractions of subsieve dry beans (DB), chickpeas (CCP), red lentils (RL), and green lentils (GL). Four samples of those legumes wereutilized as replicates and were incubated for up to 48 h in the rumen of 3 rams. RL and CCP had higher organic matter (OM) than DBand GL. Crude protein (CP) was the highest in GL (P < 0.05). Ether extract (EE) concentrations were higher in CCP and DB comparedto those of RL and GL (P < 0.05). Crude fiber (CF), acid detergent fiber (ADF), and neutral detergent fiber (NDF) contents were thehighest in RL (P < 0.05). The acid insoluble nitrogen (ADIN-N) content was similar in all samples evaluated. While RL had the lowestOM and CP degradability and water-soluble OM and CP concentrations, DB and CCP had the highest potentially degradable OM andCP contents at the end of the 48 h incubation period (P < 0.05). Escaped protein content was the highest in RL and the lowest in DB (P< 0.05). In conclusion, some of the pulse species that are produced locally and not suitable for human consumption can be used in thediet of ruminant animals as a source of protein based on OM and CP degradabilities and escape protein contents.

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1. Ertuğrul M, Dellal G, Cedden F, Akman N, Yener SM, Türkoğlu M, Elibol O, Fıratlı Ç, Gençer HV. Hayvan Yetiştirme. 1. Baskı. Eskişehir, Türkiye: Anadolu Üniversitesi Açıköğretim Fakültesi Yayını; 2011. s. 4-20 (in Turkish).
2. Kutlu HR, Gül A, Görgülü M. Türkiye Hayvancılığının Sorunları ve Çözüm Yolları; I. Damızlık Hayvan-Kaliteli Yem. Yem Magazin; Sayı: 34, s. 40-46 (in Turkish).
3. Konularına Göre İstatistikler. Türkiye İstatistik Kurumu; 2012 (in Turkish).
4. Association of Official Analytical Chemists. Official Methods of Analysis, 15th ed. Washington, DC. 1; 1990.
5. Crompton EW, Maynard LA. The relation of cellulose and lignin content to the nutritive value of animal feed. J Nutr 1938; 15: 383-395.
6. Van Soest PJ, Robertson JB. Systems of analyses for evaluation of fibrous feed. In Pigden WJ, Balch CC, Graham M, editors. Proceedings of International Workshop on Standardization of Analytical Methodology for Feeds. Ottawa, Canada: International Development Research Centre; 1979.
7. Goering HK, Van Soest PJ. Forage Fiber Analyses (Apparatus Reagents, Procedures and Some Applications). Agriculture Handbook; US Department of Agriculture, 1970.
8. Çetinkaya N. Yem maddelerinin değerlendirilmesinde naylon torba metodunun kullanılması. Yem Magazin Dergisi 1992; 1: 28-30 (in Turkish).
9. Mertens DR. Dietary components: Relationship to rate and extent of ruminal digestion. Fed Proc 1977; 36: 187-195.
10. Wechsler FS. Mathematical models for kinetics of fiber digestion and their application to tropical forages grown in controlled environments. PhD, University of Georgia, Athens, Georgia, 1981.
11. Statistical Analysis Software: Programme User Guide. Statistics (Version 5 Ed.). SAS Institute Inc. Carry, NC, USA; 1995.
12. Mullahey JJ, Walker SS, Moore KJ, Moser LE, Klopfenstein TJ. In situ ruminal protein degradation of swichgrass and smooth bromegrass. Agron J 1992; 84: 183-188.
13. Steel RG, Torrie JH. Principle and Procedures of Statistics, 2nd ed. New York, NY, USA: McGraw Hill; 1980.
14. Ensminger ME, Oldfield JE, Heinemann WW. Feeds and Nutrition. 2nd ed. Clovis, CA, USA: The 21. Özyiğit Y, Bilgen M. Bazı baklagil yem bitkilerinde farklı biçim dönemlerinin bazı kalite faktörleri üzerine etkisi. Akdeniz Üniversitesi Ziraat Fakültesi Dergisi 2006; 19: 29-34 (in Turkish). 22. Goelema JO, Spreeuwenberg MAM, Hof G, Van Der Poel AFB, Tamminga S. Effect of pressure toasting on the rumen degradability and intestinal digestibility of whole and broken peas, lupins and faba beans and a mixture of these feedstuffs. Anim Feed Sci Tech 1998; 76: 35-50. 23. Abreu JMF, Bruno-Soares AM. Chemical composition, organic matter digestibility and gas production of nine legume grains. Anim Feed Sci Tech 1998; 70: 49-57. 24. El-Niely HFG. Effect of radiation processing on antinutrients, in-vitro protein digestibility and protein efficiency ratio bioassay of legume seeds. Radiat Phys Chem 2007; 76: 1050- 1057. 25. Ragg EM, Galbusera V, Scarafoni A, Negri A, Tedeschi G, Consonni A, Sessa F, Duranti M. Inhibitory properties and solution structure of a potent Bowman-Birk protease inhibitor from lentil (lens culinaris, L) seeds. FEBS J 2006; 273: 4024- 4039. 26. Guillamon E, Pedrosa MM, Burbano C, Cuadrado C, Sanchez MC, Muzquız M. The trypsin inhibitors present in seed of different grain legume species and cultivar. Food Chem 2008; 107: 68-74. 27. Abreu JMF, Bruno-Soares AM. Characterization and utilization of rice, legume and rape straws. CIHEAM Publ 1998; 39-51. 28. Hedley C. Introduction. In: Carbohydrates in Grain Legume Seeds. 1st ed. Norwich, UK: CAB eBooks; 2000. 29. Wang M, Jiang J, Tan ZL, Tang SX, Sun ZH, Han XF. In situ ruminal crude protein and starch degradation of three classes of feedstuffs in goats. J Appl Anim Res 2009; 36: 23-28. 30. Holt NW, Soluski FW. Nonprotein nitrogen contents of some grain legumes. Can J Plant Sci 1981; 61: 515-523. 31. Seifdavati J, Taghizadeh A. Effects of moist heat treatment on ruminal nutrient degradability of and in vitro intestinal digestibility of crude protein from some of legume seeds. J Food Agric Environ 2012; 10: 390-397.Ensminger Publishing Company; 1990.
15. Hadjıpanayiotou M. Chemical composition, digestibility and in situ degradability of narbon vetch grain and straw grown in a Mediterranean region. Ann Zootech 2000; 49: 475-478.
16. Canbolat Ö, Bayram G. Bazı baklagil danelerinin in vitro gaz üretim parametreleri, sindirilebilir organik madde ve metabolik enerji içeriklerinin karşılaştırılması. U.Ü. Ziraat Fakültesi Dergisi 2007; 21: 31-42 (in Turkish).
17. Kaya F. Ülkemizde yetiştirilen bazı mercimek çeşitlerinin bileşimlerinin belirlenmesi. Yüksek Lisans Tezi, Çukurova Üniversitesi Fen Bilimleri Enstitüsü, Adana, Türkiye, 2010 (in Turkish).
18. Wang N, Daun JK. The Chemical Composition and Nutritive Value of Canadian Pulses: Lentil (Lens culinaris). Canadian Grain Commission Grain Research Laboratory. Winnipeg, Canada 2004; 8-18.
19. Çomaklı B, Yanar M, Menteşe Ö, Turgut L. Kültürel uygulamaların kaba yemlerin besleme değerine etkileri. International Animal Nutrition Congress. Isparta, Türkiye; Süleyman Demirel Üniv. Ziraat Fakültesi; 2000; s. 456-463 (in Turkish).
20. Ergün A, Tuncer ŞD, Çolpan İ, Yalçın S, Yıldız G, Küçükersan MK, Küçükersan S, Şehu A, Saçaklı P. Yemler, Yem Hijyeni ve Teknolojileri. 4. Baskı. Ankara, Türkiye: Pozitif Yayınevi; 2011 (in Turkish).
21. Özyiğit Y, Bilgen M. Bazı baklagil yem bitkilerinde farklı biçim dönemlerinin bazı kalite faktörleri üzerine etkisi. Akdeniz Üniversitesi Ziraat Fakültesi Dergisi 2006; 19: 29-34 (in Turkish).
22. Goelema JO, Spreeuwenberg MAM, Hof G, Van Der Poel AFB, Tamminga S. Effect of pressure toasting on the rumen degradability and intestinal digestibility of whole and broken peas, lupins and faba beans and a mixture of these feedstuffs. Anim Feed Sci Tech 1998; 76: 35-50.
23. Abreu JMF, Bruno-Soares AM. Chemical composition, organic matter digestibility and gas production of nine legume grains. Anim Feed Sci Tech 1998; 70: 49-57.
24. El-Niely HFG. Effect of radiation processing on antinutrients, in-vitro protein digestibility and protein efficiency ratio bioassay of legume seeds. Radiat Phys Chem 2007; 76: 1050- 1057.
25. Ragg EM, Galbusera V, Scarafoni A, Negri A, Tedeschi G, Consonni A, Sessa F, Duranti M. Inhibitory properties and solution structure of a potent Bowman-Birk protease inhibitor from lentil (lens culinaris, L) seeds. FEBS J 2006; 273: 4024- 4039.
26. Guillamon E, Pedrosa MM, Burbano C, Cuadrado C, Sanchez MC, Muzquız M. The trypsin inhibitors present in seed of different grain legume species and cultivar. Food Chem 2008; 107: 68-74.
27. Abreu JMF, Bruno-Soares AM. Characterization and utilization of rice, legume and rape straws. CIHEAM Publ 1998; 39-51.
28. Hedley C. Introduction. In: Carbohydrates in Grain Legume Seeds. 1st ed. Norwich, UK: CAB eBooks; 2000.
29. Wang M, Jiang J, Tan ZL, Tang SX, Sun ZH, Han XF. In situ ruminal crude protein and starch degradation of three classes of feedstuffs in goats. J Appl Anim Res 2009; 36: 23-28.
30. Holt NW, Soluski FW. Nonprotein nitrogen contents of some grain legumes. Can J Plant Sci 1981; 61: 515-523.
31. Seifdavati J, Taghizadeh A. Effects of moist heat treatment on ruminal nutrient degradability of and in vitro intestinal digestibility of crude protein from some of legume seeds. J Food Agric Environ 2012; 10: 390-397.