Comparison and Evaluation of the Passive Haemagglutination Test and ELISA in Routine Screening of Contagious Equine Metritis (CEM) in Mares with Endometritis or Infertility
One hundred and twenty thoroughbred breeder mares with endometritis or infertility were investigated for the detection of antibodies to Taylorella equigenitalis by the passive haemagglutination (PHA) test and ELISA between 1997 and 1999 in the Marmara region and the two tests were compared. Fifty-eight (48.33 %) of 120 sera were found to be positive by the PHA test and 52 (43.33%) by ELISA. 22 of the sera were found to be positive by the PHA test reacted at 1/64 titer, 27 at 1/128, 7 at 1/256 and 2 at 1/512 titer. Sera from 44 mares were positive in both tests. Amongst these, 14 sera were positive in the PHA test and 8 sera were positive in ELISA. The difference between the PHA test and ELISA positivity was 8% and this was not statistically significant. In conclusion, the PHA test was recommended for routine screening of CEM because of its easy handling and rapidness.
Anahtar Kelimeler:
Taylorella equigenitalis, Contagious equine metritis, passive haemagglutination test, ELISA, infertility, endometritis
Comparison and Evaluation of the Passive Haemagglutination Test and ELISA in Routine Screening of Contagious Equine Metritis (CEM) in Mares with Endometritis or Infertility
One hundred and twenty thoroughbred breeder mares with endometritis or infertility were investigated for the detection of antibodies to Taylorella equigenitalis by the passive haemagglutination (PHA) test and ELISA between 1997 and 1999 in the Marmara region and the two tests were compared. Fifty-eight (48.33 %) of 120 sera were found to be positive by the PHA test and 52 (43.33%) by ELISA. 22 of the sera were found to be positive by the PHA test reacted at 1/64 titer, 27 at 1/128, 7 at 1/256 and 2 at 1/512 titer. Sera from 44 mares were positive in both tests. Amongst these, 14 sera were positive in the PHA test and 8 sera were positive in ELISA. The difference between the PHA test and ELISA positivity was 8% and this was not statistically significant. In conclusion, the PHA test was recommended for routine screening of CEM because of its easy handling and rapidness.
Keywords:
Taylorella equigenitalis, Contagious equine metritis, passive haemagglutination test, ELISA, infertility, endometritis,
- ISSN: 1300-0128
- Yayın Aralığı: Yılda 6 Sayı
- Yayıncı: TÜBİTAK
Sayıdaki Diğer Makaleler
Photoinactivation of Buffalo Liver and Kidney Tissue Arginases and its Kinetic Properties
Effects of Copper Sulphate Administration on Body Weight Gain and Some Blood Parameters in Lambs
Tekin ŞAHİN, İbrahim ÇİMTAY, Gürbüz AKSOY
Detection of Antibodies Against Salmonella Enteritidis in Chicken Serum and Egg Yolk with ELISA
Suphi DENİZ, Şakir Doğan TUNCER, Murat DEMİREL, Oktay KAPLAN, Taylan AKSU
Kazım ŞAHİN, O. Nihat ERTAŞ, Talat GÜLER, Mehmet ÇİFTÇİ
Use of Sunflower Meal with Enzyme Mixture Substituted for Soybean Meal in Broiler Diets