Hui DONG, Jie ZHOU, Xiaodong ZHANG, Yueyun DING, Zongjun YIN, Ning ZHOU, Xiang QIN, Ning WANG

Characterization of porcine adiponectin gene (ADIPOQ) polymorphismsand their association with litter size

Litter size is one of the most important economic traits for pig production as it is directly related to production efficiency. To evaluate the effects of the adiponectin (ADIPOQ) gene on porcine litter size, we investigated ADIPOQ polymorphisms in three breeds, Wannan Black pigs, Berkshire pigs, and BW pigs (crossbred pigs produced from mating male Berkshire pigs and female Wannan Black pigs), using the PCR-SSCP method. Three SNPs (c. 178G > A, c. 1165A > G, and c. 1138G > A) were identified. In Wannan Black pigs, association analysis indicated that the c. 178G > A SNP genotype was significantly associated with an effect on total number born (TNB; P < 0.05) and the number born alive (NBA; P < 0.05) in primiparity. The c.1138G > A SNP was significantly associated with an effect on TNB (P < 0.05) and NBA (P < 0.05) in primiparity and multiparity. In addition, three of the nine possible genotype combinations, AACC, AACD, and AGCD, were found. AGCD resulted in primiparous and multiparous sows with the highest TNB. These results indicated that polymorphisms in the ADIPOQ gene could be used for marker-assisted selection programs for the genetic improvement of reproductive characteristics in pigs.

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1. Li FE, Xiong YZ, Deng CY. Major gene of reproductive traits of sow and advances of QTL gene. Pigs Poult 2003; 23: 3135.
2. Rothschild MF, Ruvinsky A. The Genetics of the Pig. Wallingford, UK: CAB International; 2010.
3. Cepica S, Masopust M, Knoll A, Rohrer GA. Linkage and RH mapping of the porcine adiponectin gene on chromosome 13. Anim Genet 2005; 36: 276277.
4. Dai MH, Xia T, Zhang GD, Chen XD, Gan L, Feng SQ, Qiu H, Peng Y, Yang ZQ. Cloning, expression and chromosome localization of porcine adiponectin and adiponectin receptors genes. Domest Anim Endocrinol 2006; 30: 8897.
5. Jacobi SK, Ajuwon KM, Weber TE, Kuske JL, Dyer CJ, Spurlock MK. Cloning and expression of porcine adiponectin, and its relationship to adiposity, lipogenesis and the acute phase response. J Endocrinol 2004; 182: 133144.
6. Palin MF, Bordignon VV, Murphy BD. Adiponectin and the control of female reproductive functions. Vitam Horm 2012; 90: 240287.
7. Kaminski T, Smolinska N, Maleszka A, Kiezun M, Dobrzyn K, Czerwinska J, Szeszko K, Nitkiewicz A. Expression of adiponectin and its receptors in the porcine hypothalamus during the oestrous cycle. Reprod Dom Anim 2014; 49: 378386.
8. Wen JP, Lv WS, Yang J, Nie AF, Cheng XB, Yang Y, Ge Y, Li XY, Ning G. Globular adiponectin inhibits GnRH secretion from GT1-7 hypothalamic GnRH neurons by induction of hyperpolarization of membrane potential. Biochem Biophys Res Commun 2008; 371: 756761.
9. Rodriguez-Pacheco F, Martinez-Fuentes AJ, Tovar S, Pinilla L, Tena-Sempere M, Dieguez C, Castano JP, Malagon MM. Regulation of pituitary cell function by adiponectin. Endocrinology 2007; 148: 401410.
10. Lu M, Tang Q, Olefsky JM, Mellon PL, Webster NJ. Adiponectin activates adenosine monophosphate-activated protein kinase and decreases luteinizing hormone secretion in LβT2 gonadotropes. Mol Endocrinol 2008; 22: 760771.
11. Houde AA, Murphy D, Mathieu O, Bordignon V, Palin F. Characterization of swine adiponectin and adiponectin receptor polymorphisms and their association with reproductive traits. Anim Genet 2008; 39: 249257.
12. Sambrook J, Russell DW. Molecular Cloning. A Laboratory Manual. 3rd ed. Cold Spring Harbor, NY, USA: Cold Spring Laboratory Press; 2001.
13. Weaver B, Wuensch KL. SPSS and SAS programs for comparing Pearson correlations and OLS regression coefficients. Behav Res Methods 2013; 45: 880895.
14. Goto Y, Yue L, Yokoi A, Nishimura R, Uehara T, Koizumi S, Saikawa Y. A novel single-nucleotide polymorphism in the 3′-untranslated region of the human dihydrofolate reductase gene with enhanced expression. Clin Cancer Res 2001; 7: 19521956.
15. Alberobello AT, Congedo V, Liu H, Cochran C, Skarulis MC, Forrest D, Celi FS. An intronic SNP in the thyroid hormone receptor β gene is associated with pituitary cell-specific over-expression of a mutant thyroid hormone receptor β2 (R338W) in the index case of pituitary-selective resistance to thyroid hormone. J Trans Med 2011; 9: 144.
16. Ross J. mRNA stability in mammalian cells. Microbiol Rev 1995; 59: 423450.
17. Gingerich TJ, Feige JJ, LaMarre J. AU-rich elements and the control of gene expression through regulated mRNA stability. Anim Health Res Rev 2004; 5: 4963.
18. Haap M, Machicao F, Stefan N, Thamer C, Tschritter O, Schnuck F, Wallwiener D, Stumvoll M, Haring HU, Fritsche A. Genetic determinants of insulin action in polycystic ovary syndrome. Exp Clin Endocrinol Diabetes 2005; 113: 275281.
19. Guo X, Liu JQ, You LP, Li G, Huang YN, Li YL. Association between adiponectin polymorphisms and the risk of colorectal cancer. Genet Test Mol Biomarkers 2015; 19: 913.
20. Morsci NS, Sellner EM, Schnabel RD, Taylor JF. Association analysis of adiponectin and somatostatin polymorphisms on BTA1 with growth and carcass traits in Angus cattle. Anim Genet 2006; 37: 554562.
21. Lan XY, Wei TB, Zhang LZ, Chen H, Hu SR, Lei CZ, Fang XT. Novel polymorphism at the 3′-UTR of the caprine adiponectin gene. Biochem Genet 2009; 47: 251256.
22. Kim ST, Marquard K, Stephens S, Louden E, Allsworth J, Moley KH. Adiponectin and adiponectin receptors in the mouse preimplantation embryo and uterus. Hum Reprod 2011; 26: 8295.
23. Klenke U, Taylor-Burds C, Wray S. Metabolic influences on reproduction: adiponectin attenuates GnRH neuronal activity in female mice. Endocrinology 2014; 155: 18511863.