Application of an indirect MilA ELISA for the detection of Mycoplasma bovis antibodies in bovine milk

Öz The objective of this study was to detect Mycoplasma bovis specific antibodies using a recently reported MilA ELISA with the aim to detect M. bovis antibodies in milk. An indirect ELISA, based on a recombinant fragment of the Mycoplasma immunogenic lipase A MilA protein, was conducted on 291 milk samples for the detection of M. bovis antibodies. Samples were also tested with conventional Mycoplasma culture and M. bovis PCR. Samples were collected from individual cows from 2 commercial dairy herds in South Australia. Of 291 samples tested, 68 23.4% were detected positive for M. bovis antibodies, 150 51.5% were positive for M. bovis in PCR and 166 57.0% in bacterial culture. These results indicate that MilA indirect ELISA can be utilized for the detection of M. bovis antibodies in milk.

Kaynakça

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Kaynak Göster

Bibtex @ { tbtkveterinary783790, journal = {Turkish Journal of Veterinary and Animal Sciences}, issn = {1300-0128}, eissn = {1303-6181}, address = {}, publisher = {TÜBİTAK}, year = {2020}, volume = {44}, pages = {752 - 755}, doi = {10.3906/vet-1811-62}, title = {Application of an indirect MilA ELISA for the detection of Mycoplasma bovis antibodies in bovine milk}, key = {cite}, author = {Farha, Abd Al Bar Al and Wawegama, Nadeeka and Hemmatzadeh, Farhıd and Fırestone, Sımon and Moffat, Jamıe and Kojourı, Gholam Alı and Ahanıazarı, Anıa and Amanollahı, Reza and Hoare, Andrew and Petrovskı, Kıro} }
APA Farha, A , Wawegama, N , Hemmatzadeh, F , Fırestone, S , Moffat, J , Kojourı, G , Ahanıazarı, A , Amanollahı, R , Hoare, A , Petrovskı, K . (2020). Application of an indirect MilA ELISA for the detection of Mycoplasma bovis antibodies in bovine milk . Turkish Journal of Veterinary and Animal Sciences , 44 (3) , 752-755 . DOI: 10.3906/vet-1811-62
MLA Farha, A , Wawegama, N , Hemmatzadeh, F , Fırestone, S , Moffat, J , Kojourı, G , Ahanıazarı, A , Amanollahı, R , Hoare, A , Petrovskı, K . "Application of an indirect MilA ELISA for the detection of Mycoplasma bovis antibodies in bovine milk" . Turkish Journal of Veterinary and Animal Sciences 44 (2020 ): 752-755 <https://dergipark.org.tr/tr/pub/tbtkveterinary/issue/56433/783790>
Chicago Farha, A , Wawegama, N , Hemmatzadeh, F , Fırestone, S , Moffat, J , Kojourı, G , Ahanıazarı, A , Amanollahı, R , Hoare, A , Petrovskı, K . "Application of an indirect MilA ELISA for the detection of Mycoplasma bovis antibodies in bovine milk". Turkish Journal of Veterinary and Animal Sciences 44 (2020 ): 752-755
RIS TY - JOUR T1 - Application of an indirect MilA ELISA for the detection of Mycoplasma bovis antibodies in bovine milk AU - Abd Al Bar Al Farha , Nadeeka Wawegama , Farhıd Hemmatzadeh , Sımon Fırestone , Jamıe Moffat , Gholam Alı Kojourı , Anıa Ahanıazarı , Reza Amanollahı , Andrew Hoare , Kıro Petrovskı Y1 - 2020 PY - 2020 N1 - doi: 10.3906/vet-1811-62 DO - 10.3906/vet-1811-62 T2 - Turkish Journal of Veterinary and Animal Sciences JF - Journal JO - JOR SP - 752 EP - 755 VL - 44 IS - 3 SN - 1300-0128-1303-6181 M3 - doi: 10.3906/vet-1811-62 UR - https://doi.org/10.3906/vet-1811-62 Y2 - 2021 ER -
EndNote %0 Turkish Journal of Veterinary and Animal Sciences Application of an indirect MilA ELISA for the detection of Mycoplasma bovis antibodies in bovine milk %A Abd Al Bar Al Farha , Nadeeka Wawegama , Farhıd Hemmatzadeh , Sımon Fırestone , Jamıe Moffat , Gholam Alı Kojourı , Anıa Ahanıazarı , Reza Amanollahı , Andrew Hoare , Kıro Petrovskı %T Application of an indirect MilA ELISA for the detection of Mycoplasma bovis antibodies in bovine milk %D 2020 %J Turkish Journal of Veterinary and Animal Sciences %P 1300-0128-1303-6181 %V 44 %N 3 %R doi: 10.3906/vet-1811-62 %U 10.3906/vet-1811-62
ISNAD Farha, Abd Al Bar Al , Wawegama, Nadeeka , Hemmatzadeh, Farhıd , Fırestone, Sımon , Moffat, Jamıe , Kojourı, Gholam Alı , Ahanıazarı, Anıa , Amanollahı, Reza , Hoare, Andrew , Petrovskı, Kıro . "Application of an indirect MilA ELISA for the detection of Mycoplasma bovis antibodies in bovine milk". Turkish Journal of Veterinary and Animal Sciences 44 / 3 (Haziran 2020): 752-755 . https://doi.org/10.3906/vet-1811-62
AMA Farha A , Wawegama N , Hemmatzadeh F , Fırestone S , Moffat J , Kojourı G , Ahanıazarı A , Amanollahı R , Hoare A , Petrovskı K . Application of an indirect MilA ELISA for the detection of Mycoplasma bovis antibodies in bovine milk. Turkish Journal of Veterinary and Animal Sciences. 2020; 44(3): 752-755.
Vancouver Farha A , Wawegama N , Hemmatzadeh F , Fırestone S , Moffat J , Kojourı G , Ahanıazarı A , Amanollahı R , Hoare A , Petrovskı K . Application of an indirect MilA ELISA for the detection of Mycoplasma bovis antibodies in bovine milk. Turkish Journal of Veterinary and Animal Sciences. 2020; 44(3): 752-755.
IEEE A. Farha , N. Wawegama , F. Hemmatzadeh , S. Fırestone , J. Moffat , G. Kojourı , A. Ahanıazarı , R. Amanollahı , A. Hoare ve K. Petrovskı , "Application of an indirect MilA ELISA for the detection of Mycoplasma bovis antibodies in bovine milk", Turkish Journal of Veterinary and Animal Sciences, c. 44, sayı. 3, ss. 752-755, Haz. 2020, doi:10.3906/vet-1811-62

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