Antibacterial activity of bryophyte species against Paenibacillus larvae isolates
This study was performed to determine the antibacterial activity of methanol extracts of 23 bryophyte species against Paenibacillus larvae isolates that cause American foulbrood diseases in honeybee larvae. The honey and larva samples were collected from nine different locations of Rize in Turkey. A total of 22 gram-positive spore-forming bacteria were isolated from the larva and honey samples. According to the results of morphological, biochemical, and molecular (16S rRNA gene sequencing) tests, 10 isolates of the 22 gram-positive spore-forming bacteria were identified as P. larvae. A total of 10 bryophyte species (Polytrichum formasum, Polytrichum commune, Calliergonella cuspitada, Calliergonella lindbergi, Metzgeria conjugata, Isothecium alopecuroides, Syntrichia calcicola, Syntrichia intermedia, Tortella densa, and Grimmia alpestris) among 23 bryophytes showed good antimicrobial activity against P. larvae isolates according the results of agar-well diffusion method and minimal inhibition concentration experiments.
___
- Evans JD. Diverse origins of tetracycline resistance in the
honeybee bacterial pathogen
Paenibacillus larvae
. J Invertebr
Pathology 2003; 83: 46-50.
- Genersch E. American Foulbrood in honeybees and its
causative agent,
Paenibacillus larvae
. J InvertebrPathol 2010;
103 (Suppl.): 10-19.
- Thompson TS, Pernal PS, Noot DK, Melathopoulos AP, van
den Heever JP. Degradation of incurred tylosin to desmycosin
– implications for residue analysis of honey. Anal Chim Acta
2007; 586: 304-311.
- Gonzalez MJ, Marioli JM. Antibacterial activity of water
extracts and essential oils of various aromatic plants against
Paenibacillus larvae
, the causative agent of American
Foulbrood. J Invertebr Pathol 2010; 104: 209-213.
- Reybroeck W, Daeseleire E, De Brabander HF, Herman L.
Antimicrobials in beekeeping. Vet Microbiol 2012; 158: 1-11.
- Gende LB, Fernandez N, Buffa F, Ruiu L, Satta A, Fritz R,
Eguaras MJ, Floris I. Susceptibility of
Paenibacillus larvae
isolates to a tetracycline hydrochloride and cinnamon
(
Cinnamomum zeylanicum
) essential oil mixture. B Insectol
2010; 63: 247-250.
- Boligon AA, Brum TF, Zadra M, Piana M, Alves CFS, Fausto
VP, Barboza Júnior VS, Vaucher RA, Santos RCV, Athayde MI.
Antimicrobial activity of
Scutia buxifolia
against the honeybee
pathogen
Paenibacillus larvae
. J Invertebr Pathol 2013; 112:
105-107.
- Scher JM, Speakman JB, Zapp J, Becker H. Bioactivity guided
isolation of antifungal compounds from the liverwort
Bazzania
trilobata
(L.) Gray SF. Phytochemistry 2004; 65: 2583-2588.
- Dülger B, Hacioğlu N, Uyar G. Evaluation of antimicrobial
activity of some mosses from Turkey. Asian J Chem 2009; 21:
4093-4096.
- Nikolajeva V, Liepina L, Petrina Z, Krumina G, Grube M,
Muiznieks I. Antibacterial activity of extracts from some
bryophytes. Advances in Microbiology 2012; 2: 345-353.
- Deora GS, Rathore MS. Antimicrobial activity of certain
bryophytes. Biosciences Biotechnology Research Asia 2013;
10: 705-710.
- Asakawa Y. Biologically active compounds from bryophytes.
Pure Appl Chem 2007; 79: 557-580.
- Sevim E, Celebi O, Sevim A. Determination of the bacterial
flora as a microbial control agent of
Toxoptera aurantii
(Homoptera: Aphididae). Biologia 67: 397-404.
- de Graaf DC, Alippi AM, Antúnez K, Aronstein KA, Budge
G, De Koker D, De Smet L, Dingman DW, Evans JD, Foster
LJ et al. Standard methods for American foulbrood research. J
Apicult Res 2013; 52: 1-27.
- Sambrook J, Fritsch EF, Maniatis T. Molecular Cloning: A
Laboratory Manual. 2nd ed. Cold Spring Harbor, NY, USA:
Cold Spring Harbor Laboratory Press; 1989.
- Demirci M, Sevim E, Demir I, Sevim A. Culturable bacterial
microbiota of
Plagiodera versicolora
(L.) (Coleoptera:
Chrysomelidae) and virulence of the isolated strains. Folia
Microbiol 2013; 58: 201-210.
- Tamura K, Peterson D, Peterson N, Stecher G, Nei M, Kumar
S. MEGA5: Molecular evolutionary genetics analysis using
maximum likelihood, evolutionary distance, and maximum
parsimony methods. Mol Biol Evol 2011; 28: 2731-2739.
- Clinical and Laboratory Standards Institute. Performance
Standards for Antimicrobial Susceptibility Testing. Fifteenth
Informational Supplement M100-S15. Wayne, PA, USA:
Clinical and Laboratory Standards Institute; 2006.
- Aghdasi M, Mofid Bojnoordi M, Mianabadi M, Nadaf M.
Chemical components of the
Epherda major
from Iran. Nat
Prod Res 2013; 30: 369-371.
- Balouiri M, Sadiki M, Ibnsouda SK. Methods for
in vitro
evaluating antimicrobial activity: a review. Journal of
Pharmaceutical Analysis 2016; 6: 71-79.
- Clinical and Laboratory Standarts Institute. Methods for
Dilution Antimicrobial Susceptibility Tests for Bacteria That
Grow Aerobically. 9th ed. Approved Standard M7-A6. Wayne,
PA, USA: Clinical and Laboratory Standards Institute; 2008.
- Dobbelaere W, Graaf DC, Peeters JE. Development of a fast and
reliable diagnostic method for American foulbrood disease
(
Paenibacillus larvae
subsp.
larvae
) using a 16S rRNA gene
based PCR. Apidologie 2001; 32: 363-370.
- Neuendorf S, Hedtke K, Tangen G, Genersch E. Biochemical
characterization of different genotypes of
Paenibacillus larvae
subsp.
larvae
, a honey bee bacterial pathogen. Microbiology
2004; 150: 2381-2390.
- Kochansky J, Knox DA, Feldlaufer M, Pettis JS. Screening
alternative antibiotics against oxytetracycline-susceptible and
-resistant
Paenibacillus larvae
. Apidologie 2001; 32: 215-222.
- Alippi AM, Leon IE, Lopez AC. Tetracycline-resistance
encoding plasmids from
Paenibacillus larvae
, the causal agent
of American foulbrood disease, isolated from commercial
honeys. Int Microbiol 2014; 17: 49-61.
- Cowan MM. Plant products as antimicrobial agents. Clin
Microbiol Rev 1999; 12: 564-582.
- Zhu RL, Wang D, Xu L, Shi RP, Wang J, Zheng M. Antibacterial
activity in extracts of some bryophytes from China and
Mongolia. J Hattori Bot Lab 2006; 100: 603-615.
- İlhan S, Savaroğlu F, Çolak F, Filik İşçen C, Erdemgil FZ.
Antimicrobial activity of
Palustriella commutata
(Hedw.)
Ochyra extracts (Bryophyta). Turk J Biol 2006; 30: 149-152.
- Savaroglu F, Ilhan S, Filik-Iscen C. An evaluation of the
antimicrobial activity of some Turkish mosses. J Med Plants
Res 2011; 5: 3286-3292.
- Elibol B, Ezer T, Kara R, Yuvalı Çelik G, Çolak E. Antifungal
and antibacterial effects of some acrocarpic mosses. Afr J
Biotechnol 1998; 10: 986-989.