Granny Smith Apple Extract Lowers Inflammation and Improves Antioxidant Status in L-arginineinduced Exocrine Pancreatic Dysfunction in Rats

Objectives: Granny Smith is a cultivated hybrid variety of apple with a high antioxidant content relative to all other species of apple. Acute pancreatitis (AP) is an instantly emerging inflammatory condition with a high mortality rate. The preferred treatment is restricted to symptomatic relief and supportive care. The present study was undertaken to evaluate the favorable effects of Granny Smith apple extract (GSAE) as a prophylactic treatment for L-arginine-induced AP in rats. Materials and Methods: Male Sprague Dawley rats were divided in to five groups (n=6): Normal control (saline), disease control (a single dose of L-arginine 2.5 g/kg I.P.), positive control (pelatonin 10 mg/kg I.P.), and GSAE I and II (200 mg/kg and 400 mg/kg, orally, respectively). All groups were treated for 7 days. At the end of the study, blood samples were collected from the retro-orbital plexus, serum separated, and subjected to estimation of biomarker enzymes such as amylase, lipase, antioxidant enzymes, etc. The animals were then sacrificed, and the pancreas was isolated and subjected to estimation of tissue biomarkers, DNA fragmentation assay, and histopathological studies. Results: Serum levels of amylase and lipase were significantly (p

Granny Smith Elma Ekstresi, Sıçanlarda L-arginin Kaynaklı Ekzokrin Pankreas Bozukluğunda İnflamasyonu Azaltır ve Antioksidan Durumunu İyileştirir

Amaç: Granny Smith, diğer elma türlerine göre yüksek antioksidan içeriğine sahip, yetiştirilmiş bir melez elma çeşididir. Akut pankreatit (AP), yüksek ölüm oranına sahip, anında ortaya çıkan bir enflamatuvar süreçtir. Tercih edilen tedavi, semptomatik rahatlama ve destekleyici bakım ile sınırlıdır. Bu çalışma, Granny Smith elma özütünün (GSAE) sıçanlarda L-arginin kaynaklı AP için profilaktik bir tedavi olarak olumlu etkilerini değerlendirmek için yapılmıştır. Gereç ve Yöntemler: Erkek Sprague Dawley sıçanları beş gruba ayrıldı (n=6): Normal kontrol (salin), hastalık kontrol (tek doz L-arginin 2,5 g/kg I.P.), pozitif kontrol (pelatonin 10 mg/kg I.P.) ve GSAE I ve II (sırasıyla 200 mg/kg ve 400 mg/kg ağızdan). Tüm gruplar 7 gün tedavi edildi. Çalışmanın sonunda, retro-orbital pleksustan kan örnekleri alındı, serum ayrıldı ve amilaz, lipaz, antioksidan enzimler gibi biyobelirteç enzimler analiz edildi. Hayvanlar daha sonra öldürüldü ve pankreas izole edildi. Doku biyobelirteçlerinin analizi, DNA fragmantasyon analizi ve histopatolojik çalışmalara tabi tutulmuştur. Bulgular: Serum amilaz ve lipaz seviyeleri, L-arginin ile tedavi edilen sıçanlarda önemli ölçüde azaldı (p

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Turkish Journal of Pharmaceutical Sciences
  • ISSN: 1304-530X
  • Yayın Aralığı: Yılda 6 Sayı
  • Başlangıç: 2000

6.6b294

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