A Preliminary Study on the Antioxidant Activity of Origanum haussknechtii Boiss.

Bu çalışmanın amacı Türkiye için endemik bir tür olan Origanum haussknechtii Boiss. (Lamiaceae)'nin toprak üstü kısımlarından hazırlanan sulu ve metanollü ekstrelerin antioksidan aktivitesinin tayin edilmesidir. Ekstreler antioksidan kapasiteleri için 2,2-difenil-1-pikrilhidrazil (DPPH) radikal süpürücü, troloks'a eşdeğer antioksidan kapasite (TEAC) ve tiyobarbitürik asit (TBA) testi ile lipid peroksidasyonun inhibisyonu bakımından değerlendirilmiştir. Ayrıca ekstrelerin total fenolik içerikleri spektrofotometrik metot ile tayin edilmiştir. Ekstrelerin kullanılan test modellerinde farklı düzeylerde antioksidan özelliklere sahip olduğu bulunmuştur. Her iki ekstrenin referanslar ile kıyaslandığında DPPH radikal süpürücü aktiviteye sahip olduğu görülmüştür, bununla birlikte metanollü ekstrenin (IC50=1.22 ± 1.25 mg/mL) daha belirgin aktiviteye sahip olduğu bulunmuştur. TEAC testinde, metanollü ekstrenin (1.35 ± 0.28 mmol trolox/g) sulu ekstreden daha yüksek antioksidan kapasiteye sahip olduğu bulunmuştur. TBA metotunda da metanollü ekstre (IC50=23.62 ± 2.42 µg/mL) propil gallat ile karşılaştırıldığında lipid peroksidasyon üzerine sulu ekstreden daha yüksek inhibitör aktivite göstermiştir. Sonuçlar O. haussknechtii'den hazırlanan metanollü ekstrenin doğal orijinli iyi bir antioksidan kaynağı olduğnu göstermiştir

Origanum haussknechtii Boiss.'nin Antioksidan Aktivitesi Üzerine Bir Ön Çalışma

The aim of this study was to determine antioxidant activity of the water and methanol extracts from the aerial parts of Origanum haussknechtii Boiss. (Lamiaceae), which is an endemic to Turkey. The water and methanol extracts of the plant were evaluated for their antioxidant capacity in 2,2-diphenyl-1picrylhydrazyl (DPPH) radical scavenging, trolox equivalent antioxidant capacity (TEAC) and inhibition of lipid peroxidation with thiobarbituric acid (TBA) assays. In addition, the total phenolic content in both extracts was determined with spectrophotometric method. The extracts were found to have different levels of antioxidant properties in the test models used. Both extracts of the plant were shown to possess DPPH radical scavenging activity compared to reference substances, but that of methanol extract (IC50=1.22 ± 1.25 mg/mL) was found to be more pronounced. In the TEAC assay, the methanol extract displayed higher antioxidant capacity (1.35 ± 0.28 mmol trolox/g) than the water extract. In the TBA method, the methanol extract (IC50=23.62 ± 2.42 µg/mL) also showed higher inhibitory activity on the lipid peroxidation than the water extract when compared to propyl gallate. The results revealed that the methanol extract of O. haussknechtii is a good antioxidant source of natural origin

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Turkish Journal of Pharmaceutical Sciences
  • ISSN: 1304-530X
  • Yayın Aralığı: Yılda 6 Sayı
  • Başlangıç: 2000

7b314

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