Yavuz ÖZORAN, Etem ALHAN, Hayrettin ÇIRAY, Yavuz TEKELİOĞLU, Ümit ÇOBANOĞLU

The Significance of DNA Ploidy in the Malignant Potential of Colorectal Adenocarcinomas

Aim: The aim of this study was to evaluate the association between flow cytometric DNA ploidy and the potential for invasiveness and lymph node metastasis in colorectal adenocarcinomas. Materials and Methods: The study included 40 colorectal carcinoma cases that were examined with flow cytometry and light microscopy. DNA ploidy in 4 different areas (deep tumor tissue, superficial tumor tissue, normal adjacent mucosa, and lymph node metastasis) was analyzed with flow cytometry and compared with invasiveness and lymph node metastasis Results: Statistically significant differences in DNA content were observed between the deep and superficial layers of tumor tissues. The rate of aneuploidy was similar in the deep region of the tumors and lymph node metastases. Conclusions: The results suggest that the level of aneuploidy observed in the deep invasive region of tumors was associated with lymph node metastases.

Anahtar Kelimeler:

Colorectal adenocarcinoma, DNA ploidy

The Significance of DNA Ploidy in the Malignant Potential of Colorectal Adenocarcinomas

Keywords:

Colorectal adenocarcinoma, DNA ploidy,

PDF

___

1. Salud A, Porcel JM, Raikundalia B, Camplejohn RS, Taub NA. Tumor angiogenesis and mast cell density in the prognostic assessment of colorectal carcinomas. Dig Liver Dis 2005; 37: 162-169.
2. Eminovic-Behrem S, Trobonja Z, Petrovecki M, Dobi-Babic R, Dujmovic M, Jonjic N. Prognostic significance of DNA ploidy pattern and nucleolar organizer regions (AgNOR) in colorectal carcinoma. Croat Med J 2000; 41: 154-158.
3. Kouri M, Pyrhonen S, Mecklin JP, Jarvinen H, Laasonen A, Franssila K et al. The prognostic value of DNA-ploidy in colorectal carcinoma: a prospective study. Br J Cancer 1990; 62: 976-981.
4. Lanza G, Gafa R, Santini A, Marstri I, Dubini A, Gilli G et al. Prognostic significance of DNA ploidy in patients with stage II and stage III colon carcinoma. Cancer 1998; 82: 49-59.
5. Quirke P, Dixon MF, Clayden AD, Durdey P, Dyson JE, Williams NS et al. Prognostic significance of DNA aneuploidy and cell proliferation in rectal adenocarcinomas. J Pathol 1987; 151: 285-291.
6. Rognum TO, Lund E, Meling GI, Langmark F. Near diploid large bowel carcinomas have better five-year survival than aneuploid ones. Cancer 1991; 68: 1077-1081.
7. Heimann TM, Miller F, Martinelli F, Mester J, Kurtz RJ, Szporn A et al. Significance of DNA content abnormalities in small rectal cancers. Am J Surg 1990; 159: 199-203.
8. Karelia NH, Patel DD, Desai NS, Mehta HV, Yadav PK, Patel SM et al. Prognostic significance of DNA aneuploidy and p21 ras oncoprotein expression in colorectal cancer and their role in the determination of treatment modalities. Int J Biol Markers 2001; 16: 97-104.
9. Kim YJ, Ngoi SS, Godwin TA, DeCosse JJ, Staiano-Coico L. Ploidy in invasive colorectal cancer. Implicatins for metastatic disease. Cancer 1991; 68: 638-641.
10. Lin JK, Chang SC, Yang SH, Jiang JK, Chen WC, Lin TC. Prognostic value of DNA ploidy patterns of colorectal adenocarcinoma. Hepatogastroenterology 2003; 50: 1927- 1932.
11. Tang R, Ho YS, You YT, Hsu KC, Chen JS, Changchien CR et al. Prognostic evaluation of DNA flow cytometric and histopathologic parameters of colorectal cancer. Cancer 1995; 76: 1724-1730.
12. Visscher DW, Zarbo RJ, Ma CK, Sakr WA, Crissman JD. Flow cytometric DNA and clinicopathologic analysis of Dukes’ A&B colonic adenocarcinomas: a retrospective study. Mod Pathol 1990; 3: 709-712.
13. Zarbo RJ, Nakhleh RE, Brown RD, Kubus JJ, Ma CK, Mackowiak P. Prognostic significance of DNA ploidy and proliferation in 309 colorectal carcinomas as determined by two-color multiparametric DNA flow cytometry. Cancer 1997; 79: 2073- 2086.
14. Jass JR, Sobin LH. World Health Organization international histological classification of tumors: histological typing of intestinal tumors. 2nd ed. New York: Springer-Verlag; 1989.
15. Bazan V, Migliavacca M, Zanna I, Tubiolo C, Corsale S, Calo V et al. DNA ploidy and S-phase fraction, but not p53 or NM23-H1 expression, predict outcome in colorectal cancer patients. Result of a 5-year prospective study. J Cancer Res Clin Oncol 2002; 128: 650-658.
16. Giaretti W, Danova M, Geido E, Mazini G, Sciallero S, Aste H. Flow cytometric DNA index in the prognosis of colorectal cancer. Cancer 1991; 67: 1921-1927.
17. Thomas GDH, Dixon MF, Smeeton NC, Williams NS: Observer variation in the histologic grading of rectal carcinoma. J Clin Pathol 1983; 36: 385-391.
18. Böttger TC, Potraiz D, Stöckle M, Wellek S, Klupp J, Junginger T. Prognostic value of DNA analysis in colorectal carcinoma. Cancer 1993; 71: 3579-3587.
19. Emdin SO, Stenling R, Roos G. study with some methodological aspects. Cancer 1987; 60: 1282-1287.
20. Baretton G, Gille J, Oevermann E, Lohrs U. Flow-cytometric analysis of the DNA-content in paraffin-embedded tissue from colorectal carcinomas and its prognostic significance. Virchows Arch B Cell Pathol Inc Mol Pathol 1991; 60: 123-311.
21. Dean PA, Vernava AM III. Flow cytometric analysis of DNA content in colorectal carcinoma. Dis Colon Rectum 1992; 35: 95-102.
22. Chen HS, Chen SMS, Chang C. DNA index and S-phase fraction in curative resection of colorectal adenocarcinoma: Analysis of prognosis and current trends. World J Surg 2002; 26: 626-630.
23. Tonouchi H, Matsumoto K, Kinoshita T, Itoh H, Suzuki H. Prognostic value of DNA ploidy patterns of colorectal adenocarcinoma: Univariate and Multivariate Analysis. Dig Surg 1998; 15: 687-692.
24. Pinto AE, Chaves P, Fidalgo P. Flow cytometric DNA ploidy and Sphase fraction correlate with histopathologic indicators of tumor behavior in colorectal carcinoma. Dis Colon Rectum 1997; 40: 411-419.
25. Scott NA, Wieand HS, Moertel CG, Cha SS, Beart RW, Lieber MM. Colorectal cancer: Dukes’ stage, tumor site, preoperative plasma CEA level, and patient prognosis related to tumor DNA ploidy pattern. Arch Surg 1987; 122: 1375-1379.
26. Rognum TO, Thorud E, Lund E. Survival of large bowel carcinoma patients with different DNA ploidy. Br J Cancer 1987; 56: 633- 636.
27. Armitage NC, Ballantyne KC, Sheffield JP, Clarke P, Evans DF, Hardcastle JD. A prospective evaluation of the effect of tumor cell DNA content on recurrence in colorectal cancer 1991; 67: 2599- 2604.
28. Jones DJ, Moore M, Schofield PF. Refining the prognostic significance of DNA ploidy status in colorectal cancer. Int J Cancer 1988; 41: 206-210.
29. Giovagnoli MR, Giarnieri E, Midiri G, Tesoriere A, Ferraro S, Vecchione A. Intratumoral heterogeneity in colorectal carcinoma: trucut sampling for DNA ploidy analysis. Anticancer Res 1999; 19 (5C): 4577-4580.
30. Saccani Jotti G, Fontanesi M, Orsi N, Sarli L, Pietra N, Peracchia A et al. DNA content in human colon cancer and non-neoplastic adjacent mucosa. Int J Biol Markers 1995; 10: 11-16.