Tuğba KOCA, Mustafa AKÇAM, Meltem ÖZGÖÇMEN, Nurcan DOGAN, Duygu KUMBUL DOĞUÇ

The protective effect of Capparis ovata in acute hepatotoxicity induced by paracetamol

Background/aim: To investigate the efficiency of Capparis ovata as a protective agent against acute paracetamol toxicity of the liver. Materials and methods: A total of 36 Wistar albino rats were divided into 4 groups: 1) paracetamol, 2) Capparis ovata + paracetamol, 3) Capparis ovata, and 4) control. Groups 2 and 3 were given Capparis ovata and Groups 1 and 4 distilled water for 8 days. On day 8, 3000 mg kg 1 paracetamol was administered orally to Groups 1 and 2. Samples were taken on day 9. AST, ALT, total bilirubin, direct bilirubin, GGT, and ALP levels were assessed. Lipid peroxidation markers and thiobarbituric acid-reactive substance (TBARS) levels were measured in the blood and liver. Liver tissues were evaluated histologically. Results: AST, ALT, and total bilirubin levels were lower in Group 2 than in Group 1 (P < 0.05). TBARS levels were lower in Groups 2 (P = 0.000), 3 (P = 0.001), and 4 (P = 0.001) than in Group 1. Degenerative findings were lower in the Capparis ovata + paracetamol group than in the paracetamol group (P < 0.05). Conclusion: It can be concluded that Capparis ovata has a protective effect on the liver, both histopathologically and biochemically, against paracetamol-induced liver injury

PDF

___

1. Donnelly PJ, Walker RM, Racz WJ. Inhibition of mitochondrial respiration in vivo is an early event in acetaminophen-induced hepatotoxicity. Arch Toxicol 1994; 68: 110118.
2. Dargan PI, Jones AL. Management of paracetamol poisoning. Trends Pharmacol Sci 2003; 24: 154157.
3. Germanò MP, De Pasquale R, DAngelo V, Catania S, Silvari V, Costa C. Evaluation of extracts and isolated fraction from Capparis spinosa L. buds as an antioxidant source. J Agric Food Chem 2002; 50: 11681171.
4. Kamalakkannan N, Stanely Mainzen Prince P. Rutin improves the antioxidant status in streptozotocin-induced diabetic rat tissues. Mol Cell Biochem 2006; 2293: 211219.
5. Proestos C, Boziaris IS, Nychas GJE, Komaitis M. Analysis of flavonoids and phenolic acids in Greek aromatic plants: investigation of their antioxidant capacity and antimicrobial activity. Food Chem 2006; 5: 664671.
6. Tülümen T, Ayata A, Özen M, Sütçü R, Canatan D. The protective effect of Capparis ovata on 6-mercaptopurineinduced hepatotoxicity and oxidative stress in rats. J Pediat Hematol Oncol 2015; 37: 290294.
7. Baravalia Y, Chanda S. Protective effect of Woodfordia fruticosa flowers against acetaminophen-induced hepatic toxicity in rats. Pharm Biol 2011; 49: 826832.
8. Abdel-Wahhab MA, Nada SA, Arbid MS. Ochratoxicosis: prevention of developmental toxicity by L -methionine in rats. J Appl Toxicol 1999; 19: 712.
9. Andıran N, Sarıkayalar F. Pattern of acute poisonings in childhood in Ankara: what has changed in twenty years? Turk J Pediatr 2004; 46: 147152.
10. Jones A. Over-the-counter analgesics: atoxicologic perspective. Am J Ther 2002; 9: 245257.
11. Mintegi S, Fernández A, Alustiza J, Canduela V, Mongil I, Caubet I, Clerigué N, Herranz M, Crespo E, Fanjul JL et al. Emergency visits for childhood poisoning: a 2-year prospective multicenter survey in Spain. Pediatr Emerg Care 2006; 22: 334338.
12. Gadgoli C, Mishra S. Antihepatotoxic activity of p-methoxy benzoic acid from Capparis spinosa. J Ethnopharmacol 1999; 6: 187192.
13. Siracusa L, Kulisic-Bilusic T, Politeo O, Krause I, Dejanovic B, Ruberto G. Phenolic composition and antioxidant activity of aqueous infusions from Capparis spinosa L. and Crithmum maritimum L. before and after submission to a two-step in vitro digestion model. J Agric Food Chem 2011; 59: 1245312459.
14. Bonina F, Puglia C, Ventura D, Aquino R, Tortora S, Sacchi A, Saija A, Tomaino A, Pellegrino ML, de Caprariis P. In vitro antioxidant and in vivo photoprotective effects of a lyophilized extract of Capparis spinosa L. buds. J Cosmet Sci 2002; 53: 321 335.
15. Baijal R, Patel N, Kolhapure SA. Evaluation of efficacy and safety of Liv.52 DS tablets in acute viral hepatitis: a prospective, double-blind, randomized, placebo-controlled, phase III clinical trial. Medicine Update 2004; 12: 4153.
16. Goyal R, Grewal RB. The influence of teent (Capparis decidua) on human plasma triglycerides, total lipids and phospholipids. Nutr Health 2003; 17: 7176.
17. Madhavan V, Pandey AS, Murali A, Yoganarasimhan SN. Protective effects of Capparis sepiaria root extracts against acetaminophen-induced hepatotoxicity in Wistar rats. J Complement Med 2012; 9: 112.
18. Anju Aniyathi MJ, Latha PG, Manikili P, Suja SR, Shyamal S, Shine VJ, Sini S, Anuja GI, Shikha P, Vidyadharan MK et al. Evaluation of hepatoprotective activity of Capparis brevispina DC. stem bark. IJNPR 2009; 8: 514519.
19. Kumar Mishra S, Singh PN, Dubey SD. Evaluation of antioxidant activities in ethanolic extract of Capparis Zeylanica Linn. root. Rev Lat Quim 2013; 1: 720.
20. Aghel N, Rashidi I, Mombeini A. Hepatoprotective activity of Capparis spinosa root bark against CCl4 induced hepatic damage in mice. Iran J Pharm Res 2007; 6: 285290.