Özlem Güzel TUNÇCAN, AYŞE KALKANCI, Elif Ayça ÜNAL, Olkar ABDULMAJED, Merve ERDOĞAN, MURAT DİZBAY, Kayhan ÇAĞLAR

The in vitro effect of antimicrobial photodynamic therapy on Candida and Staphylococcus biofilms

Öz Background/aim: This study was designed to evaluate the effect of antimicrobial photodynamic treatment (APDT) in a biofilm model using combinations of various dyes (rose bengal, riboflavin, and methylene blue) as photosensitizers and light sources (LED and UVA) against staphylococcal and candidal biofilms. Materials and methods: Sterile microtiter plates were used for the development and quantification of the biofilms. APDT was carried out using combinations of the light sources and dyes. The percentage of the growth inhibition was then calculated using a spectrophotometer. The broth media in the wells were aspirated, wells were stained with crystal violet, and optical density values were measured spectrophotometrically. SEM analysis of the impact of APDT on bacterial and fungal biofilms was also performed. Results: The experiments showed that the most efficacious combination was red LED + methylene blue against both staphylococcal and candidal biofilms. A marked inhibition (45.4%) was detected on both C. albicans and C. parapsilosis biofilms. Red LED + methylene blue was also effective on S. aureus and S. epidermidis biofilms. SEM images suggested that the number of adherent cells and biofilm mass were markedly reduced after APDT treatment. Conclusion: Although the results of this study indicated the in vitro efficacy of APDT, it might also be a promising technique for the control of biofilm growth within intravenous catheters.

Anahtar Kelimeler:

Antimicrobial photodynamic therapy, biofilm, Candida, Staphylococcus

Tam Metin

Kaynak Göster

Bibtex	`@ { tbtkmedical576743, journal = {Turkish Journal of Medical Sciences}, issn = {1300-0144}, eissn = {1303-6165}, address = {}, publisher = {TÜBİTAK}, year = {2018}, volume = {48}, pages = {873 - 879}, doi = {}, title = {The in vitro effect of antimicrobial photodynamic therapy on Candida and Staphylococcus biofilms}, key = {cite}, author = {Tunçcan, Özlem Güzel and Kalkancı, AYŞE and Ünal, Elif Ayça and Abdulmajed, Olkar and Erdoğan, Merve and Dizbay, MURAT and Çağlar, Kayhan} }`
APA	Tunçcan, Ö , Kalkancı, A , Ünal, E , Abdulmajed, O , Erdoğan, M , Dizbay, M , Çağlar, K . (2018). The in vitro effect of antimicrobial photodynamic therapy on Candida and Staphylococcus biofilms . Turkish Journal of Medical Sciences , 48 (4) , 873-879 . Retrieved from https://dergipark.org.tr/tr/pub/tbtkmedical/issue/45702/576743
MLA	Tunçcan, Ö , Kalkancı, A , Ünal, E , Abdulmajed, O , Erdoğan, M , Dizbay, M , Çağlar, K . "The in vitro effect of antimicrobial photodynamic therapy on Candida and Staphylococcus biofilms" . Turkish Journal of Medical Sciences 48 (2018 ): 873-879 <https://dergipark.org.tr/tr/pub/tbtkmedical/issue/45702/576743>
Chicago	Tunçcan, Ö , Kalkancı, A , Ünal, E , Abdulmajed, O , Erdoğan, M , Dizbay, M , Çağlar, K . "The in vitro effect of antimicrobial photodynamic therapy on Candida and Staphylococcus biofilms". Turkish Journal of Medical Sciences 48 (2018 ): 873-879
RIS	TY - JOUR T1 - The in vitro effect of antimicrobial photodynamic therapy on Candida and Staphylococcus biofilms AU - Özlem Güzel Tunçcan , AYŞE Kalkancı , Elif Ayça Ünal , Olkar Abdulmajed , Merve Erdoğan , MURAT Dizbay , Kayhan Çağlar Y1 - 2018 PY - 2018 N1 - DO - T2 - Turkish Journal of Medical Sciences JF - Journal JO - JOR SP - 873 EP - 879 VL - 48 IS - 4 SN - 1300-0144-1303-6165 M3 - UR - Y2 - 2021 ER -
EndNote	%0 Turkish Journal of Medical Sciences The in vitro effect of antimicrobial photodynamic therapy on Candida and Staphylococcus biofilms %A Özlem Güzel Tunçcan , AYŞE Kalkancı , Elif Ayça Ünal , Olkar Abdulmajed , Merve Erdoğan , MURAT Dizbay , Kayhan Çağlar %T The in vitro effect of antimicrobial photodynamic therapy on Candida and Staphylococcus biofilms %D 2018 %J Turkish Journal of Medical Sciences %P 1300-0144-1303-6165 %V 48 %N 4 %R %U
ISNAD	Tunçcan, Özlem Güzel , Kalkancı, AYŞE , Ünal, Elif Ayça , Abdulmajed, Olkar , Erdoğan, Merve , Dizbay, MURAT , Çağlar, Kayhan . "The in vitro effect of antimicrobial photodynamic therapy on Candida and Staphylococcus biofilms". Turkish Journal of Medical Sciences 48 / 4 (Ağustos 2018): 873-879 .
AMA	Tunçcan Ö , Kalkancı A , Ünal E , Abdulmajed O , Erdoğan M , Dizbay M , Çağlar K . The in vitro effect of antimicrobial photodynamic therapy on Candida and Staphylococcus biofilms. Turkish Journal of Medical Sciences. 2018; 48(4): 873-879.
Vancouver	Tunçcan Ö , Kalkancı A , Ünal E , Abdulmajed O , Erdoğan M , Dizbay M , Çağlar K . The in vitro effect of antimicrobial photodynamic therapy on Candida and Staphylococcus biofilms. Turkish Journal of Medical Sciences. 2018; 48(4): 873-879.
IEEE	Ö. Tunçcan , A. Kalkancı , E. Ünal , O. Abdulmajed , M. Erdoğan , M. Dizbay ve K. Çağlar , "The in vitro effect of antimicrobial photodynamic therapy on Candida and Staphylococcus biofilms", Turkish Journal of Medical Sciences, c. 48, sayı. 4, ss. 873-879, Ağu. 2018