Osman ÇAĞLAYAN, Üçler KISA, M. Murad BAŞAR, Mehmet FERHAT

Seminal plasma transforming growth factor- B (TGF- B) and epidermal growth factor (EGF) levels in patients with varicocele

Amaç: Bu çalışmanın amacı varikoselli infertil hastalar ile normal fertil olgular arasında seminal plazma EGF ve TGF-p düzeylerini karşılaştırmak, ve seminal plazma EGF ve TGF-P düzeyleri, semen parametreleri ve serum hormon düzeyleri arasındaki ilişkiyi incelemektir. Materyal ve Metot: Varikoselli 100 infertil hasta (Grup-1) çalışmaya alındı. İki ile 5 günlük cinsel perhiz sonrası semen analizi uygulandı. Semen analiz sonuçlarına göre bu hastalar oligoastenoeratozoospermisi olanlar (Grup-1) ve normal semen analizi olanlar (Grup-2) olarak iki gruba ayrıldı. İlave olarak, skrotal patolojisi olmayan 100 fertil olgu kontrol grubu (Grup-3) olarak kabul edildi ve semen analizi bunlarda da uygulandı. Seminal plazma EGF ve TGF-p düzeyleri ELİZA yöntemi ile ölçüldü. Ayrıca, tüm olgularda serum gonadotropin ve seks steroid düzeyleri ölçüldü, istatistiskel analiz tek yönlü ANOVA test ve post hoc Bonferoni test ile yapıldı. Bulgular: Her iki grup arasında semen analizi parametreleri bakımından anlamlı farklık vardı (P < 0,05). Seminal plazma EGF ve TGF-p düzeyleri varikoselli olgularda (Grup-1 ve Grup-2) Grup-3'den daha yüksekti. Ancak, istatistiksel fark sadece TGF-p düzeylerinde saptandı (P = 0,017). Seminal plazma EGF ve TGF-P seminal parametreler ile negatik ilişki göstermekteydi (P < 0,05). Bununa karşın, bu büyüme faktörleri ile serum hormon düzeyleri arasında ilişki, yoktu. Sonuç: Bu çalışma göstermektedir ki, seminal plasma EGF ve TGF-P düzeylerindeki artış varikoselli hastalarda seminal parametrelerde azalma ile birliktedir. Bununla birilkte, etki TGF-p ile daha belirgindir.

Varikoselli hastalarda seminal plazma transforme edici büyüme faktörü-B (TGF-B) ve epidermal büyüme faktörü (EGF) düzeyleri

Aims: The aim of this study was to analyze seminal plasma epidermal growth factor (EGF) and transforming growth factor-B (TGF-J3) levels in infertile patients with varicocele and normal fertile cases, and to evaluate the relationships between seminal plasma EGF and TGF-p, seminal parameters and serum hormone levels. Materials and Methods: A total of 100 patients with varicocele were enrolled into the study. Semen analyses were performed after 2 and 5 days of sexual abstinence. These patients were divided into two groups based on the results of semen analysis as oligoasthenoteratozoospermia (Group 1) and normal semen analysis (Group 2). Additionally, 100 fertile cases without any scrotal pathology were accepted as the control group (Group 3), and semen analysis was also performed in these cases. Seminal plasma EGF and TGF-P levels were measured with ELISA. Additionally, serum gonadotropin and serum sex steroid levels were measured in patients and controls. Statistical analysis was performed by using one-way ANOVA test and post hoc •Bonferroni test. Results: There was a statistically significant difference between patients and controls regarding seminal parameters (P < 0.05). Seminal plasma EGF and TGF-p levels were higher in patients with varicocele (Group 1 and Group 2) than Group 3; however, there was a statistically significant difference only in TGF-P levels (P = 0.017). Seminal plasma EGF and TGF-p levels showed a negative relationship with seminal parameters (P < 0.005). However, there was no relationship between these growth factors and serum hormone levels. Conclusions: This study showed that increased levels of EGF and TGF-p' were associated with decreased seminal parameters in patients with varicocele. However, the relationship was more evident with TGF-p.

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