S1PR2 deficiency enhances neuropathic pain induced by partial sciatic nerve ligation

Öz Background/aim: Sphingosine 1-phosphate receptor 2 (S1PR2), a member of the seven-transmembrane receptor family, can be activated by its natural ligand sphingosine 1-phosphate (S1P) to initiate signal transduction and is involved in a wide range of biological effects such as immune cell migration and vascular permeability. Its relationship with neuropathic pain (NP) has not been reported. In this study, the effects of S1PR2 on the development of NP were studied.Materials and methods: We generated a model of NP by partial sciatic nerve ligation (pSNL). The 50% paw withdrawal threshold of the wild-type (WT) group and the S1PR2 deficiency group were measured at several time points after surgery. The inflammatory factor levels of the two groups were measured by real-time quantitative polymerase chain reaction (RT-PCR). Neutrophil infiltration and glial cell activation were detected by immunofluorescence. Matrix metalloproteinase 9 (MMP9) and its substrate myelin basic protein (MBP) were measured by RT-PCR, western blotting, and immunofluorescence.Result: The S1PR2 deficiency group showed a reduction in 50% paw withdrawal threshold compared with WT mice (P < 0.05) at 3 days after the operation. In the ligated sciatic nerve of the S1PR2 deficiency group, the mRNA expression of IL-1ß was increased; the numbers of infiltrating neutrophils and activated astrocytes were also increased. The expression of MMP9 was elevated while MBP was decreased. Conclusion: S1PR2 deficiency could increase the pain sensitivity of a NP mouse model and promote the development of NP.

Kaynak Göster

Bibtex @ { tbtkmedical577835, journal = {Turkish Journal of Medical Sciences}, issn = {1300-0144}, eissn = {1303-6165}, address = {}, publisher = {TÜBİTAK}, year = {2019}, volume = {49}, pages = {412 - 421}, doi = {}, title = {S1PR2 deficiency enhances neuropathic pain induced by partial sciatic nerve ligation}, key = {cite}, author = {Gu, Qiang and Hou, Jin Chao and Fang, Xiang Ming} }
APA Gu, Q , Hou, J , Fang, X . (2019). S1PR2 deficiency enhances neuropathic pain induced by partial sciatic nerve ligation . Turkish Journal of Medical Sciences , 49 (1) , 412-421 . Retrieved from https://dergipark.org.tr/tr/pub/tbtkmedical/issue/45750/577835
MLA Gu, Q , Hou, J , Fang, X . "S1PR2 deficiency enhances neuropathic pain induced by partial sciatic nerve ligation" . Turkish Journal of Medical Sciences 49 (2019 ): 412-421 <https://dergipark.org.tr/tr/pub/tbtkmedical/issue/45750/577835>
Chicago Gu, Q , Hou, J , Fang, X . "S1PR2 deficiency enhances neuropathic pain induced by partial sciatic nerve ligation". Turkish Journal of Medical Sciences 49 (2019 ): 412-421
RIS TY - JOUR T1 - S1PR2 deficiency enhances neuropathic pain induced by partial sciatic nerve ligation AU - Qiang Gu , Jin Chao Hou , Xiang Ming Fang Y1 - 2019 PY - 2019 N1 - DO - T2 - Turkish Journal of Medical Sciences JF - Journal JO - JOR SP - 412 EP - 421 VL - 49 IS - 1 SN - 1300-0144-1303-6165 M3 - UR - Y2 - 2021 ER -
EndNote %0 Turkish Journal of Medical Sciences S1PR2 deficiency enhances neuropathic pain induced by partial sciatic nerve ligation %A Qiang Gu , Jin Chao Hou , Xiang Ming Fang %T S1PR2 deficiency enhances neuropathic pain induced by partial sciatic nerve ligation %D 2019 %J Turkish Journal of Medical Sciences %P 1300-0144-1303-6165 %V 49 %N 1 %R %U
ISNAD Gu, Qiang , Hou, Jin Chao , Fang, Xiang Ming . "S1PR2 deficiency enhances neuropathic pain induced by partial sciatic nerve ligation". Turkish Journal of Medical Sciences 49 / 1 (Şubat 2019): 412-421 .
AMA Gu Q , Hou J , Fang X . S1PR2 deficiency enhances neuropathic pain induced by partial sciatic nerve ligation. Turkish Journal of Medical Sciences. 2019; 49(1): 412-421.
Vancouver Gu Q , Hou J , Fang X . S1PR2 deficiency enhances neuropathic pain induced by partial sciatic nerve ligation. Turkish Journal of Medical Sciences. 2019; 49(1): 412-421.
IEEE Q. Gu , J. Hou ve X. Fang , "S1PR2 deficiency enhances neuropathic pain induced by partial sciatic nerve ligation", Turkish Journal of Medical Sciences, c. 49, sayı. 1, ss. 412-421, Şub. 2019

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