Hatice BAŞ, Özlem KARA, Mustafa KARA, Dilek PANDIR

Protective effect of vardenafil on ischemia–reperfusion injury in rat ovary

To investigate the effect of vardenafil on ischemia–reperfusion (I/R) injury in rat ovary. Materials and methods: A total of 21 Wistar rats were divided into 3 groups. In group 1, ovary torsion was not performed and no drug was administered. In group 2, 1 h of ischemia and 2 h of reperfusion were performed and no drug was given. In group 3, 1 h of ischemia and 2 h of reperfusion were performed and 1 mg/kg vardenafil was administered after 30 min of ischemia. Right ovaries were surgically removed in all groups. Malondialdehyde (MDA) levels and activities of superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPx) were measured. Apoptosis protease-activating factor 1 (Apaf-1) was measured by using immunohistochemistry. Results: MDA level was significantly increased and SOD, CAT, and GPx activities were significantly decreased in group 2 compared to the other groups (P < 0.05). In light microscopic investigations, severe vascular congestion, edema, hemorrhage, and follicular degeneration were assessed in the ovary tissue. Ovarian tissue damage was higher in group 2 than group 3. Apaf-1 expression was stronger in group 2 than group 3. Conclusion: Vardenafil treatment seems to reduce microscopic histopathological changes, decrease Apaf-1 response, and alter oxidative stress in I/R injury in rat ovaries.

Anahtar Kelimeler:

Key words: Ovarian torsion, ischemia–reperfusion, vardenafil, antioxidant enzymes, apoptosis

Protective effect of vardenafil on ischemia–reperfusion injury in rat ovary

Keywords:

Key words: Ovarian torsion, ischemia–reperfusion, vardenafil, antioxidant enzymes, apoptosis,

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Becker JH, Jan de Graaff J, Vosb CM. Torsion of the ovary: a known but frequently missed diagnosis. Eur J Emerg Med 2009; 16: 124–6.
Halici Z, Karaca M, Keles ON, Borekci B, Odabasoglu F, Suleyman H et al. Protective effects of amlodipine on ischemia-reperfusion injury of rat ovary: biochemical and histopathologic evaluation. Fertil Steril 2008; 90: 2408–15.
Kara M, Daglioglu YK, Kuyucu Y, Tuli A, Tap A. The effect of edaravone on ischemia–reperfusion injury in rat ovary. Eur J Obstet Gynecol Reprod Biol 2012; 162: 197–202.
Abramov AY, Scorziello A, Duchen MR. Three distinct mechanisms generate oxygen free radicals in neurons and contribute to cell death during anoxia and reoxygenation. J Neurosci 2007; 27: 1129–38.
Demircioğlu Rİ, Usta B, Sert H, Muslu B, Gözdemir M. Taurine is protective against oxidative stress during cold ischemia in the rat kidney. Turk J Med Sci 2011; 41: 843–9.
Özdemir B, Kaya A, Söğüt Ö, Kaya H, Gökdemir MT, Celbiş O. Oxidative stress status of individuals involved in traffic accidents. Turk J Med Sci 2012; 42: 507–14.
Kalender S, Kalender Y, Durak D, Ogutcu A, Uzunhisarcikli M, Cevrimli BS. Methyl parathion induced nephrotoxicity in male rats and protective role of vitamins C and E. Pestic Biochem Physiol 2007; 88: 213–8.
Parlakpinar H, Sahna E, Acet A, Mizrak B, Polat A. Protective effect of caffeic acid phenethyl ester (CAPE) on myocardial ischemia–reperfusion induced apoptotic cell death. Toxicol 2005; 209: 1–14.
Kalender Y, Kaya S, Durak D, Uzun FG, Demir F. Protective effects of catechin and quercetin on antioxidant status, lipid peroxidation and testis histoarchitecture induced by chlorpyrifos in male rats. Environ Toxicol Phar 2012; 33: 141–
Çömelekoğlu Ü, Yalın S, Ballı E, Berköz M. Ovariectomy decreases biomechanical quality of skin via oxidative stress in rat. Turk J Med Sci 2012; 42: 201–9.
Sayın O, Arslan N, Altun ZS, Akdoğan G. In vitro effect of resveratrol against oxidative injury of human coronary artery endothelial cells. Turk J Med Sci 2011; 41: 211–8.
Hajra KM, Liu JR. Apoptosome dysfunction in human cancer. Apoptosis 2004; 9: 691–704.
Watson AJM. Apoptosis and colorectal cancer. Gut 2004; 53: 1701–9.
Shabsigh R. Therapy of ED: PDE-5 inhibitors. Endocrine 2004; 23: 135–41.
Zhang RL, Zhang Z, Zhang L, Wang Y, Zhang C, Chopp M. Delayed treatment with sildenafil enhances neurogenesis and improves functional recovery in aged rats after focal cerebral ischemia. J Neurosci Res 2006; 15;83: 1213–9.
Hascalik S, Celik O, Turkoz Y, Mizrak B. Clip Turcica: a new apparatus for experimental ovarian ischemia and reperfusion model in rats. Fertil Steril 2005; 84: 219–20.
Aebi H. Catalase in vitro. Methods Enzymol 1984; 105: 121–6.
Paglia DE, Valentine WN. Studies on the quantitative and qualitative characterization of glutathione peroxidase. J Lab Med 1987; 70: 158–65.
Marklund S, Marklund G. Involvement of the superoxide anion radical in the autoxidation of pyrogallol and a convenient assay for superoxide dismutase. Eur J Biochem 1974; 47: 469–74.
Ohkawa H, Ohishi N, Yagi K. Assay for lipid peroxides in animal tissues by thiobarbituric acid rection. Anal Biochem 1979; 95: 351–8.
Lowry OH, Rosebrough NJ, Farr AL, Randall RJ. Protein measurement with the Folin phenol reagent. J Biol Chem 1951; 193: 265–75.
Obermaier R, Benz S, Von Dobschuetz E, Drognitz O, Schareck W, Jonas L et al. Characterization of microcirculatory disturbance in a novel model of pancreatic ischemiareperfusion using intravital fluorescence-microscopy. Pancreas 2002; 25: 142–8.
Soydan G, Sokmensuer C, Kilinc K, Tuncer M. The effects of sildenafil on the functional and structural changes of ileum induced by intestinal ischemia-reperfusion in rats. Eur J Pharmacol 2009; 610: 87–92.
Erol B, Tokgoz H, Hanci V, Bektas S, Akduman B, Yencilek F. Vardenafil reduces testicular damage following ischemia/ reperfusion injury in rats. Kaohsiung J Med Sci 2009; 25: 374–
Arikan DC, Bakan V, Kurutas EB, Sayar H, Coskun A. Protective effect of tadalafil on ischemia/reperfusion injury of rat ovary. J Pediatr Surg 2010; 45: 2203–9.
Oral A, Odabasoglu F, Halici Z, Keles ON, Unal B, Coskun AK et al. Protective effects of montelukast on ischemia-reperfusion injury in rat ovaries subjected to torsion and detorsion: biochemical and histopathologic evaluation. Fertil Steril 2011; 95: 1360–6.
Yigiter M, Halici Z, Odabasoglu F, Keles ON, Atalay F, Unal B et al. Growth hormone reduces tissue damage in rat ovaries subjected to torsion and detorsion evaluation. Eur J Obstet Gynecol Reprod Biol 2011; 157: 94–100.
Bayir Y, Karagoz Y, Karakus E, Albayrak A, Sengul O, Can I et al. Nigella sativa reduces tissue damage in rat ovaries subjected to torsion and detorsion: oxidative stress, proinflammatory response and histopathological evaluation. Gynecol Obstet Invest 2012; 74: 41–9.
Osmanağaoğlu M, Kesim M, Yuluğ E, Menteşe A, Karahan CS. The effect of high dose methylprednisolone on experimental ovarian torsion/reperfusion injury in rats. Geburtsh Frauenheilk 2012; 72: 70–4.
Robles R, Tao XJ, Trbovich AM, Maravel DV, Nahum R, Perez GI et al. Localization, regulation and possible consequences of apoptotic protease-activating factor-1 (Apaf-1) expression in granulosa cells of the mouse ovary. Endocrinology 1999; 140: 2641–4.