Yüksel ONARAN, Esra Aktepe KESKİN, Zehra Candan İltemir DUVAN

Prohepcidin in maternal circulation: is it related to spontaneous preterm labor?

To investigate whether spontaneous preterm labor (PTL) with intact membranes is associated with changes in maternal serum prohepcidin concentrations. Materials and methods: The study consisted of patients with spontaneous PTL with intact membranes (n = 25), a control group of healthy pregnant women between the 24th and 37th gestational weeks (n = 22), and uncomplicated term pregnancies in spontaneous labor (n = 19). Blood samples were collected from patients at the time of clinical diagnosis. Levels of prohepcidin, hemoglobin, serum ferritin, serum iron, unsaturated iron binding capacity, total iron binding capacity, transferrin and transferrin saturation, C reactive protein, and interleukin-6 were measured. Results: Patients with spontaneous PTL had significantly lower maternal serum prohepcidin concentrations than term delivery and control subjects. Conclusion: Maternal serum prohepcidin concentration is lower in patients with spontaneous PTL compared to term delivery and control subjects. This suggests that measuring maternal serum prohepcidin concentrations in PTL may be a feasible method for understanding etiologic causes of spontaneous preterm delivery, but, before suggesting this as a course of action, low levels of prohepcidin in patients with PTL need to be more fully investigated.

Anahtar Kelimeler:

Spontaneous preterm labor, term pregnancy, prohepcidin

Prohepcidin in maternal circulation: is it related to spontaneous preterm labor?

Keywords:

Spontaneous preterm labor, term pregnancy, prohepcidin,

PDF

___

Nemeth E, Valore EV, Territo M, Schiller G, Lichtenstein A, Ganz T. Hepcidin, a putative mediator of anemia of inflammation, is a type II acute-phase protein. Blood 2003; 101: 2461–2463.
Nicolas G, Chauvet C, Viatte L, Danan JL, Bigard X, Devaux I, Beaumont C, Kahn A, Vaulont S. The gene encoding the iron regulatory peptide hepcidin is regulated by anemia, hypoxia, and inflammation. J Clin Invest 2002; 110: 1037–1044.
Lee P, Peng H, Gelbart T, Wang L, Beutler E. Regulation of hepcidin transcription by interleukin-1 and interleukin-6. Proc Natl Acad Sci USA 2005; 102: 1906–1910.
Yapakçi E, Tarcan A, Celik B, Ozbek N, Gürakan B. Serum pro-hepcidin levels in term and preterm newborns with sepsis. Pediatr Int 2009; 51: 289–292.
Pitiphat W, Gillman MW, Joshipura KJ, Williams PL, Douglass CW, Rich-Edwards JW. Plasma C-reactive protein in early pregnancy and preterm delivery. Am J Epidemiol 2005; 162: 1108–1113.
Sozmen S, Mungan T, Micozkadioglu SD, Tapisiz OL. Predictive value of maternal serum and vaginal interleukin-6 levels in preterm labor. J Soc Gynecol Investig 2005; 12: 1–6.
Plevyak MP, Lambert-Messerlian GM, Farina A, Groome NP, Canick JA, Silver HM. Concentrations of serum total activin A and inhibin A in preterm and term labor patients: a cross- sectional study. J Soc Gynecol Investig 2003; 10: 231–236.
Petersen LK, Skajaa K, Uldbjerg N. Serum relaxin as a potential marker for preterm labour. Br J Obstet Gynaecol 1992; 92: 292–295.
Roe MA, Spinks C, Heath AL, Harvey LJ, Foxall R, Wimperis J, Wolf C, Fairweather-Tait SJ. Serum pro-hepcidin concentration: no association with iron absorption in healthy men; and no relationship with iron status in men carrying HFE mutations, hereditary haemochromatosis patients undergoing phlebotomy treatment, or pregnant women. Br J Nutr 2007; 97: 544–549.
Alexander GR, Himes JH, Kaufman RB, Mor J, Kogan M. A United States national reference for fetal growth. Obstet Gynecol 1996; 87: 163–168.
Halliwell B, Gutteridge JMC. Free Radicals in Biology and Medicine. 3rd ed. New York, NY, USA: Oxford University Press; 1999.
Gambling L, Dunford S, Wallace DI, Zuur G, Solanky N, Srai SK, McArdle HJ. Iron deficiency during pregnancy affects post- natal blood pressure in the rat. J Physiol 2003; 552: 603–610.
O’Brien KO, Zavaleta N, Abrams SA, Caulfield LE. Maternal iron status influences iron transfer to the fetus during the third trimester of pregnancy. Am J Clin Nutr 2003; 77: 924–930.
Nathan DG, Oski FA. Hematology of Infancy and Childhood. 4th ed. Philadelphia, PA, USA: Saunders; 1993.
De Domenico I, Ward DM, Langelier C, Vaughn MB, Nemeth E, Sundquist WI, Ganz T, Musci G, Kaplan J. The molecular mechanism of hepcidin mediated ferroportin down-regulation. Mol Biol Cell 2007; 18: 2569–2578.
Krause A, Neitz S, Mägert HJ, Schulz A, Forssmann WG, Schulz-Knappe P, Adermann K. LEAP-1, a novel highly disulfide bonded human peptide, exhibits antimicrobial activity. FEBS Lett 2000; 480: 147–150.
Muehlenbachs A, Fried M, Lachowitzer J, Mutabingwa TK, Duffy PE. Genome wide expression analysis of placental malaria reveals features of lymphoid neogenesis during chronic infection. J Immunol 2007; 179: 557–565.
McArdle HJ, Andersen HS, Jones H, Gambling L. Copper and iron transport across the placenta: regulation and interactions. J Neuroendocrinol 2008; 20: 427–431.
Nemeth E, Tuttle MS, Powelson J, Vaughn MB, Donovan A, Ward DM, Ganz T, Kaplan J. Hepcidin regulates cellular iron efflux by binding to ferroportin and inducing its internalization. Science 2004; 306: 2090–2093.
Ganz T. Hepcidin in iron metabolism. Curr Opin Hematol 2004; 11: 251–254.
Kulaksiz H, Gehrke SG, Janetzko A, Rost D, Bruckner T, Kallinowski B, Stremmel W. Pro-hepcidin: expression and cell specific localisation in the liver and its regulation in hereditary haemochromatosis, chronic renal insufficiency, and renal anaemia. Gut 2004; 53: 735–743.
Rams L, Drozdowska-Rams L, Malyszko J, Pawlak K, Malyszko J. Prohepcidin in pregnancy: acute phase protein, marker of iron metabolism, or both? Eur J Gynecol Reprod Biol 2008; 140: 293–294.
Ulukol B, Orhon FS, Hanoluk A, Akar N. Serum pro-hepcidin levels and relationship with ferritin in healthy non-anaemic infants. Acta Haematol 2007; 118: 70–72.
Tiker F, Celik B, Tarcan A, Kilicdag H, Ozbek N, Gurakan B. Serum pro-hepcidin levels and relationship with iron parameters in healthy preterm and term newborns. Pediatr Hematol Oncol 2006; 23: 293–297.
Ganz T, Olbina G, Girelli D, Nemeth E, Westerman M. Immunoassay for human hepcidin. Blood 2008; 112: 4292– 4297.
Grebenchtchikov N, Geurts-Moespot AJ, Kroot JJ, den Heijer M, Tjalsma H, Swinkels DW, Sweep FG. High-sensitive radioimmunoassay for human serum hepcidin. Br J Haematol 2009; 146: 316–325.