Microalbuminuria is considered to be an early indicator of diabetic nephropathy. We have previously reported the establishment of A-HAlb/98 hybridoma secreting monoclonal antibody that specifically recognizes human serum albumin (HSA). In this report, an in-house enzyme-linked immunosorbent assay (ELISA) was developed using this antibody for the measurement of urinary albumin (UA) and UA was examined in 24 different samples. A-HAlb/98 was used as the capture antibody by immobilizing onto microtitre plates in ELISA format. HSA-specific polyclonal rabbit immunoglobulin was labelled with biotin and then used as the tracer antibody. The dynamic assay range of ELISA for albumin was established to be 10-1000 ng/ml, and the albumin levels determined by ELISA were well correlated (r = 0.8831) with those obtained by the liquid-phase immunoprecipitation method (The Orion Diagnostica TURBOX® U-albumin assay). The ability of this in-house ELISA to quantitate UA in nanogram ranges makes it a favourable candidate for utilization in diagnostic applications as well as in research studies.

Microalbuminuria is considered to be an early indicator of diabetic nephropathy. We have previously reported the establishment of A-HAlb/98 hybridoma secreting monoclonal antibody that specifically recognizes human serum albumin (HSA). In this report, an in-house enzyme-linked immunosorbent assay (ELISA) was developed using this antibody for the measurement of urinary albumin (UA) and UA was examined in 24 different samples. A-HAlb/98 was used as the capture antibody by immobilizing onto microtitre plates in ELISA format. HSA-specific polyclonal rabbit immunoglobulin was labelled with biotin and then used as the tracer antibody. The dynamic assay range of ELISA for albumin was established to be 10-1000 ng/ml, and the albumin levels determined by ELISA were well correlated (r = 0.8831) with those obtained by the liquid-phase immunoprecipitation method (The Orion Diagnostica TURBOX® U-albumin assay). The ability of this in-house ELISA to quantitate UA in nanogram ranges makes it a favourable candidate for utilization in diagnostic applications as well as in research studies.