Tekin GÜNEY, Murat ALIŞIK, Sema AKINCI, Salim NEŞELİOĞLU, İmdat DİLEK, Özcan EREL

Evaluation of oxidant and antioxidant status in patients with vitamin B12 deficiency

Background/aim: The aim of this study was to examine the theory that oxidative stress might have an important mediating effect on the deleterious results of vitamin B12 metabolism deficiency seen throughout the body. Materials and methods: Forty patients with vitamin B12 deficiency and 40 healthy controls were included in the study. Venous blood samples were collected from all participants to evaluate serum vitamin B12, homocysteine, methylmalonic acid, total antioxidant status (TAS), and total oxidant status (TOS) levels in the pre- and posttreatment periods. Results: There were no significant differences in TAS, TOS, or oxidative stress index (OSI) levels between the pretreatment patient and control groups, and there were no significant differences in TAS or TOS levels between the posttreatment and control groups. In addition, there were no significant differences between the pre- and posttreatment TAS, TOS, and OSI levels of the patients. Conclusion: These findings are remarkable in that cyanocobalamin treatment had no direct effect on oxidant and antioxidant status in patients with vitamin B12 deficiency. The fact that there were no differences in oxidant and antioxidant status between the patients and the controls might suggest that oxidative stress does not play a role in the systemic negative effects of vitamin B12 deficiency.

Anahtar Kelimeler:

Vitamin B12, homocysteine, oxidative stress, total antioxidant status, total oxidant status

Evaluation of oxidant and antioxidant status in patients with vitamin B12 deficiency

Keywords:

Vitamin B12, homocysteine, oxidative stress, total antioxidant status, total oxidant status,

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Tefferi A, Pruthi RK. The biochemical basis of cobalamin deficiency. Mayo Clin Proc 1994; 69: 181–186.
Allen RH, Stabler SP, Savage DG, Lindenbaum J. Metabolic abnormalities in cobalamin (vitamin B12) and folate deficiency. FASEB J 1993; 7: 1344–1353.
Scalabrino G, Veber D, Mutti E. New pathogenesis of the cobalamin-deficient neuropathy. Med Secoli 2007; 19: 9–18.
Scalabrino G, Peracchi M. New insights into the pathophysiology of cobalamin deficiency. Trends Mol Med 2006; 12: 247–254.
Antony AC. Megaloblastic anemias. In: Hoffman R, Benz E, Silberstein L, Heslop H, Weitz J, Anastasi J, editors. Hematology: Basic Principles and Practice. 6th ed. Philadelphia, PA, USA: Elsevier; 2013. pp. 473–504.
Rogers EJ, Chen S, Chan A. Folate deficiency and plasma homocysteine during increased oxidative stress. N Engl J Med 2007; 357: 421–422. 7. Sibrian-Vazquez M, Escobedo JO, Lim S, Samoei GK, Strongin RM. Homocystamides promote free-radical and oxidative damage to proteins. P Natl Acad Sci USA 2010; 107: 551–554.
Al-Maskari MY, Waly MI, Ali A, Al-Shuaibi YS, Ouhtit A. Folate and vitamin B12 deficiency and hyperhomocysteinemia promote oxidative stress in adult type 2 diabetes. Nutrition 2012; 28: e23–26. 9. Erel O. A novel automated method to measure total antioxidant response against potent free radical reactions. Clin Biochem 2004; 37: 112–119.
Erel O. A new automated colorimetric method for measuring total oxidant status. Clin Biochem 2005; 38: 1103–1111.
Guney E, Fatih Ceylan M, Tektas A, Alisik M, Ergin M, Goker Z, Senses Dinc G, Ozturk O, Korkmaz A, Eker S et al. Oxidative stress in children and adolescents with anxiety disorders. J Affect Disorders 2014; 156: 62–66.
Herrmann W, Schorr H, Purschwitz K, Rassoul F, Richter V. Total homocysteine, vitamin B12, and total antioxidant status in vegetarians. Clin Chem 2001; 47: 1094–1101.
Aghamohammadi V, Gargari BP, Aliasgharzadeh A. Effect of folic acid supplementation on homocysteine, serum total antioxidant capacity, and malondialdehyde in patients with type 2 diabetes mellitus. J Am Coll Nutr 2011; 30: 210–215.
Qureshi GA, Qureshi AA, Devrajani BR, Chippa MA, Syed SA. Is the deficiency of vitamin B12 related to oxidative stress and neurotoxicity in Parkinson’s patients? CNS Neurol Disord-Dr 2008; 7: 20–27.