Mehmet Derya DEMİRAĞ, Mehmet Akif ÖZTÜRK, Zeliha TUFAN KOÇAK, İbrahim Celalettin HAZNEDAROĞLU

Effects of Ankaferd hemostat on the synovial fluid of patients with osteoarthritis

Ankaferd Blood Stopper (ABS) is a novel topical hemostatic agent with antiinfective, wound healing, and antineoplastic properties. In this study, we evaluated the macroscopic and biochemical effects of ABS in the joint fluid of patients with osteoarthritis. Materials and methods: Synovial fluid samples were obtained from 10 patients with osteoarthritis (5 women, 5 men; mean age 57.6 ± 11.2 years). From each patient, 4 mL of synovial fluid was drawn and put into 2 different serum separator tubes, and 1 mL of ABS was added to the first sample and 1 mL of 0.9% saline was added to the second sample. Macroscopic evaluation and biochemical analyses for glucose, protein, albumin, and lactate dehydrogenase (LDH) were performed. Results: The addition of ABS into synovial fluid revealed a rapid and apparent reaction resulting in dense coagulation within seconds in all of the samples. No reaction was observed in the saline group. Total protein, albumin, globulin, and LDH levels significantly decreased in the ABS group, while glucose levels remained unchanged. Conclusion: ABS can work in the synovial fluid, causes rapid frozen gel-like solidification, and decreases the protein component of the synovial fluid. This observation, together with hemostatic and wound healing properties of ABS, might provide an important starting point for testing its actions on distinct hemorrhagic, neoplastic, or inflammatory joint disorders.

Anahtar Kelimeler:

Key words: Ankaferd Blood Stopper (ABS), synovial effusion, osteoarthritis

Effects of Ankaferd hemostat on the synovial fluid of patients with osteoarthritis

Keywords:

Key words: Ankaferd Blood Stopper (ABS), synovial effusion, osteoarthritis,

PDF

___

1. Beyazit Y, Kurt M, Kekilli M, Goker H, Haznedaroglu IC. Evaluation of hemostatic eff ects of Ankaferd as an alternative medicine. Altern Med Rev 2010; 15: 329-36.
2. Haznedaroglu BZ, Haznedaroglu IC, Walker SL, Bilgili H, Goker H, Kosar A et al. Ultrastructural and morphological analyses of the in vitro and in vivo hemostatic eff ects of Ankaferd Blood Stopper. Clin Appl Th romb Hemost 2010; 16: 446-53.
3. Haznedaroglu IC. Molecular basis of the pleiotropic eff ects of Ankaferd Blood Stopper. IUBMB Life 2009; 61: 290.
4. Goker H, Haznedaroglu IC, Ercetin S, Kirazli S, Akman U, Ozturk Y et al. Haemostatic actions of the folkloric medicinal plant extract, Ankaferd blood stopper. J Int Med Res 2008; 36: 163-70.
5. Bilgili H, Kosar A, Kurt M, Onal IK, Goker H, Captug O et al. Hemostatic effi cacy of Ankaferd Blood Stopper in a swine bleeding model. Med Princ Pract 2009; 18: 165-9.
6. Huri E, Akgül KT, Yücel MO, Astarcı HM, Üstün H, Germiyanoğlu RC. Th e second step in vitro trial of Ankaferd® Bloodstopper®: comparison with other hemostatic agents. Turk J Med Sci 2011; 41: 7-15.
7. Tasdelen Fisgin M, Tanrıverdi Cayci Y, Coban AY, Ozatli D, Tanyel E, Durupinar B et al. Antimicrobial activity of plant extract Ankaferd Blood Stopper. Fitoterapia 2009; 80: 48-50.
8. Akkoc N, Akcelik M, Haznedaroglu I, Goker H, Aksu S, Kirazli S et al. In vitro antibacterial activities of Ankaferd Blood Stopper. Int J Lab Hematol 2008; 30:95.
9. Isler SC, Demircan S, Cakarer S, Cebi Z, Keskin C, Soluk M et al. Eff ects of folk medicinal plant extract Ankaferd Blood Stopper on early bone healing. J Appl Oral Sci 2010; 18: 409-14.
10. Coban YK, Ozerol E, Tanber K, Erbatur S, Aytekin AH, Fırat C. Hemeostatic effi cacy of Ankaferd aft er excision of full thickness burns: a comparative experimental study in rats. Surgical Science 2011; 2: 16-21.
11. Göker H, Çetinkaya D, Kılıç E, Haznedaroğlu IC, Kirazlı S, Fırat H. Anti-cancer activity of Ankaferd blood stopper on osteosarcom (SAOS-2) cell lines in vitro. In: Haznedaroğlu I.C. GH, Özdemir O, Koşar A, Fırat H, editors. Ankaferd: Scientifi c perspectives and basic-clinical data. İstanbul: Naviga Publications; 2008. p.109.
12. Göker H, Kılıc E, Çetinkaya D, Haznedaroğlu IC, Kirazlı S, Fırat H. Anti-cancer activity of Ankaferd on human colon cancer (CACO-2) in vitro. In: Haznedaroğlu IC, Göker H, Özdemir O et al., editors. Ankaferd: Scientifi c Perspectives and Basic-Clinical Data. İstanbul: Naviga Publications; 2008. p.108.
13. Karaman K, Celep B, Bostanci EB, Teke Z, Ulas M, Dincer N et al. Eff ects of Ankaferd Blood Stopper on pancreatic fl uid: an in vitro study. ANZ J Surg 2010; 80: 946-7
14. Freemont AJ, Denton J. Synovial fl uid analysis. In: Hochberg MC, Silman AJ, Smolen JS, Weinblatt ME, Weisman MH, editors. Rheumatology. Elsevier Mosby, 5th ed, Philadelphia 2011. p.267-72.
15. Schumacher HR. Synovial fl uid analysis and synovial biopsy. In: Ruddy S, Harris ED, Sledge B, editors. Kelley’s textbook of rheumatology. W.B. Saunders Company, 6th ed, Philadelphia 2001. p.605-19.
16. Cipil HS, Kosar A, Kaya A, Uz B, Haznedaroglu IC, Goker H et al. In vivo hemostatic eff ect of the medicinal plant extract Ankaferd Blood Stopper in rats pretreated with warfarin. Clin Appl Th romb Hemost 2009; 15: 270-6.
17. Kosar A, Cipil HS, Kaya A, Uz B, Haznedaroglu IC, Goker H et al. Th e effi cacy of Ankaferd Blood Stopper in antithrombotic drug-induced primary and secondary hemostatic abnormalities of a rat-bleeding model. Blood Coagul Fibrinolysis 2009; 20: 185-90.
18. Zulfi kar OB, Emiroglu HH, Kebudi R. Nasogastric application of topical Ankaferd Blood Stopper for bleeding from primary esophageal adenocarcinoma in a child with disseminated intravascular coagulation. Dig Liver Dis 2011; 43: 247-8.
19. Öner AF, Doğan M, Kaya A, Sal E, Bektas MS, Yesilmen O et al. New coagulant agent (Ankaferd blood stopper) for open hemorrhages in hemophilia with inhibitor. Clin Appl Th romb Hemost 2010; 16: 705-7.
20. Canatan D, Savas Ç, Kubulu AE, Anıl H, Duman H, Gürtuna A. RFVIIA and Ankaferd use in a hemophilia patient with inhibitor (abstract). 34th National Congress of Haematology. İzmir, Turkey; 2008: Abstract B056.
21. Baykul T, Alanoglu EG, Kocer G. Use of Ankaferd Blood Stopper as a hemostatic agent: a clinical experience. J Contemp Dent Pract 2010; 11: E088-E094.
22. Çalıskan Ü, Uçar Albayrak C, Acıpayamlı C. Stopping of bleeding aft er tooth extraction with Ankaferd administration in a case with TAR syndrome (abstract). 34th National Congress of Haematology. İzmir, Turkey; 2008: Abstract B0139.
23. Çalıskan Ü, Uçar Albayrak C, Acıpayamlı C. Stopping of bleeding aft er tooth extraction and circumcision with Ankaferd administration in a case with Glanzmann thrombasthenia (abstract). 34th National Congress of Haematology. İzmir, Turkey; 2008: Abstract B0140.
24. Blanchette VS. Prophylaxis in the haemophilia population. Haemophilia 2010; 16 Suppl 5: 181-8.