Dynamic thiol/disulfide homeostasis as oxidative stress marker in diabetic ketoacidosis
Dynamic thiol/disulfide homeostasis as oxidative stress marker in diabetic ketoacidosis
Background/aim: The aim of present study was to investigate the dynamic thiol/disulfide homeostasis as oxidative stress marker in diabetic ketoacidosis (DKA). Materials and methods: A total of 77 participants consisting of 32 patients with DKA and 45 healthy volunteers were included in the study. Thiol/disulfide homeostasis (TDH) [total thiol-native thiol/disulfide changes] were measured in both groups (patient group and control group) using a brand new method developed by Erel and Neselioglu. Half of the difference between total thiol and native thiol concentrations gives the amount of disulfide bond. Results: Total thiol, native thiol, and disulfide levels in blood were found to be low. The levels of total thiol (P < 0.001) and native thiol (P < 0.001 ) were significantly lower in patients with DKA than in the control group. At the same time, the level of disulfide was nonsignificantly lower in the patient group than the control group (P = 0.388). The level of IMA was higher in the patient group than in the control group (P < 0.001). Conclusion: The total thiol, native thiol, and disulfide levels in DKA decrease in favor of oxidative stress.Key words: Diabetic ketoacidosis, thiol/disulfide homeostasis, oxidative stress
___
- 1. Jain SK, McVie R, Bocchini JA Jr. Hyperketonemia (ketosis), oxidative stress and type1diabetes. Pathophysiology 2006; 13: 163-170. doi: 10.1016/j.pathophys.2006.05. 0 05
- 2. Sen CK, Packer L. Thiol homeostasis and supplements in physical exercise. Thiol homeostasis and supplements in physical exercise. The American Journal of Clinical Nutrition. 2000; 72: 653-669. doi: 10.1093/ajcn/72.2.653S
- 3. Turell L, Rafael R, Alvarez B. The thiol pool in human plasma: the central contribution of albumin to redox processes. Free Radical Biology and Medicine. 2013; 65: 244-253. doi: 10.1016/j.freeradbiomed.2013.05.050
- 4. Biswas S, Chida AS, Rahman I. Redox modifications of protein-thiols: emerging roles in cell signaling. Biochemical Pharmacology. 2006; 71: 551-564. doi : 10.1016 /j.bcp. 2005.10.044
- 5. Circu ML, Aw TY. Reactive oxygen species, cellular redox systems and apoptosis. Free Radical Biology and Medicine. 2010; 48: 749-762. doi: 10.1016/j.Freeradbiomed. 2009.12.022
- 6. Ellman G, Lysko H.A precise method for the determination of whole blood and plasma sulfhydryl groups. Analytical Biochemistry 1979; 93: 98-102.
- 7. Erel O, Neselioglu S. A novel and automated assay for thiol/ disulphide homeostasis. Clinical Biochemistry 2014; 7: 326- 332. doi: 10.1016/j.clinbiochem.2014.09.026
- 8. Ergin M, Cendek BD, Neselioglu S, Avsar AF, Erel O. Dynamic thiol-disulfide homeostasis in hyperemesis gravidarum. Journal of Perinatology 2015; 35: 788-792. doi: 10.1038/ jp.2015.81
- 9. Otal Y, Demircan S, Şener A, Alışık M, Tanrıverdi F et al. Acute renal failure and thiol-disulfide homeostasis. Journal of Nephrology & Therapeutics 2018; 8: 312. doi: 10.4172/2161- 0959.1000312
- 10. Erel Ö, Erdoğan S. Thiol-disulfide homeostasis: an integrated approach with biochemical and clinical aspects. Turkish Journal of Medical Sciences. 2020; 50: 1728-1738. doi: 10.3906/ sag-2003-64
- 11. Ercan Haydar FG, Otal Y, Şener A, Pamukçu Günaydın G, Içme F et al. The thiol-disulphide homeostasis in patients with acute pancreatitis and its relation with other blood parameters. Turkish Journal of Trauma and Emergency Surgery 2020; 26: 37-42. doi: 10.14744/tjtes.2019.38969
- 12. Ates I, Kaplan M, Yuksel M, Mese D, Alisik M et al. Determination of thiol/disulphide homeostasis in type 1 diabetes mellitus and the factors associated with thiol oxidation. Endocrine. 2016; 51: 47-51. doi: 10.1007/s12020- 015-0784-6
- 13. Tangvarasittichai S. Oxidative stress, insulin resistance, dyslipidemia and type 2 diabetes mellitus. World Journal of Diabetes 2015; 6: 456-480. doi: 10.4239/wjd.v6.i3.456
- 14. Ates I, Kaplan M, Inan B, Alisik M, Erel O et al. How does thiol/disulphide homeostasis change in prediabetic patients?Diabetes Research and Clinical Practice. 2015; 110: 166-171. doi: 10.1016/j.diabres.2015.09.011
- 15. Rains JL, Jain SK. Oxidative stress, insulin signaling, and diabetes. Free Radical Biology & Medicine 2011; 1: 567-575. doi: 10.1016/j.freeradbiomed.2010.12.006
- 16. Yuan T, Yang T, Chen H, Fu D, Hu Y et al. New insights into oxidative stress and inflammation during diabetes mellitusaccelerated atherosclerosis. Redox Biology 2019; 20: 247-260. doi: 10.1016/j.redox.2018.09.025
- 17. Gerber PA, Rutter GA. The role of oxidative stress and hypoxia in pancreatic beta-cell dysfunction in diabetes mellitus. antioxidants & redox signaling. 2017; 26: 501-518. doi: 10.1089/ars.2016.6755. Epub 2016 Jun 30
- 18. Mertoğlu C, Gunay M, Siranli G, Kulhan M, Gok G et al. The effect of the 50 g glucose challenge test on the thiol/disulfide homeostasis in pregnancy. Fetal and Pediatric Pathology 2018; 37: 147-156. doi: 10.1080/15513815.2018.1453002
- 19. Köseoğlu H, Alışık M, Başaran M, Yürekli ÖT, Solakoğlu T et al. Dynamic thiol/disulphide homeostasis in acute pancreatitis. Turkish Journal of Gastroenterology 2018; 29: 348-353. doi: 10.5152/tjg.2018.17499
- 20. Kundi H, Ates I, Kiziltunc E, Cetin M, Cicekcioglu H et al. A novel oxidative stress marker in acute myocardial infarction; thiol/disulphide homeostasis. American Journal of Emergency Medicine 2015; 33: 1567-1571. doi: 10.1016/j.ajem.2015.06.016
- 21. Ayar G, Sahin S, Yazici MU, Neselioglu S, Erel O et al. effects of hemodialysis on thiol-disulphide homeostasis in critically ill pediatric patients with acute kidney injury. BioMed Research International 2018: 1898671. doi: 10.1155/2018/1898671
- 22. Asfuroğlu EK, Boz S, Erel Ö, Neşelioğlu S, Kalkan Ç et al. Thiol/disulfide homeostasis and ischemia modified albumin levels in autoimmune gastritis and their relations with gastric emptying. Turkish Journal of Medical Sciences 2020; 50: 163- 170. doi: 10.3906/sag-1902-17
- 23. Kilinc F, Akbas A, Sener S, Ergin M, Baran P et al. The effect of tinea versicolor on thiol/disulphide homeostasis. Advances in Dermatology and Allergology 2018; 35: 299-303. doi: 10.5114/ ada.2018.76227
- 24. Sebeková K, Klenovicsová K, Ferenczová J, Hedvig J, Podracká L et al. Advanced oxidation protein products and advanced glycation end products in children and adolescents with chronic renal insufficiency. Journal of Renal Nutrition 2012; 22: 143-148. doi: 10.1053/j.jrn.2011.10.022
- 25. Yang XB, Hou FF, Wu Q, Zhou H, Liu Z et al. Increased levels of advanced oxidation protein products are associated with atherosclerosis in chronic kidney disease. Zhonghua Nei Ke Za Zhi 2005; 44: 342-346
- 26. Ozben S, Huseyinoglu N, Hanikoglu F, Guvenc TS, Yildirim BZ et al. Advanced oxidation protein products and ischaemiamodified albumin in obstructive sleep apnea.European Journal of Clinical Investigation. 2014; 44: 1045-1052. doi: 0.1111/ eci.12338
- 27. Erdogan HK, Bulur I, Kocaturk E, Saracoglu ZN, Alatas O et al. Advanced oxidation protein products and serum total oxidant/antioxidant status levels in rosacea. Advances in Dermatology and Allergology 2018; 35: 304-308. doi: 10.5114/ ada.2018.76228
- 28. Hazini A, Cemek M, Işıldak I, Alpdağtaş S, Önül A et al. Investigation of ischemia modified albumin, oxidant and antioxidant markers in acute myocardial infarction. Advances in Interventional Cardiology 2015; 11: 298-303. doi: 10.5114/ pwki.2015.55600
- 29. Hacker M, Hoyer HX, la Fougère C, Akcakoyunlu E, Schuhmann C et al. Effects of peripheral vascular intervention on ischemiamodified albumin. Coronary Artery Disease 2007; 18: 375-379. doi: 10.1097/MCA.0b013e3281ac2094
- 30. Cichota LC, Moresco RN, Duarte MM, Da Silva JEP. Evaluation of ischemia-modified albumin in anemia associated to chronic kidney disease. Journal of Clinical Laboratory Analysis. 2008; 22: 1-5. doi: 10.1002/jcla.20226