Determining the insulin secretion potential for certain specific G-protein coupled receptors in MIN6 pancreatic beta cells

Öz Background/aim: The polypeptide hormone insulin is essential for the maintenance of whole-body fuel homeostasis, and defects in insulin secretion and/or action are associated with the development of type 1 and type 2 diabetes. The aim of this study was to assess the role of some G-protein coupled receptors (GPCRs), GPR54, GPR56, and GPR75, and cannabinoid receptors CB1R and CB2R, in the regulation of pancreatic ß-cell function.Materials and methods: Insulin secretion from mouse insulinoma ß-cell line (MIN6) monolayers was assessed via insulin radioimmu-noassay (RIA). Reverse transcription-polymerase chain reaction (RT-PCR) was used to assess the expression of some specific GPCRs and the other receptors by MIN6 pancreatic ß-cells. Results: The agonists were not found to be toxic for the MIN6 pancreatic ß-cells within the range of the doses used in this study, whereas insulin secretion altered depending on the ligands and receptors. In addition, arachidonyl-2'-chloroethylamide (ACEA), carbachol, chemokine (C-C motif ) ligand-5 (CCL5), and exendin as well as phorbol myristate acetate (PMA) ligands showed significant increases in the insulin secretion of MIN6 pancreatic ß-cells.Conclusion: Understanding the normal ß-cell function and identifying the defects in ß-cell function that lead to the development of diabetes will generate new therapeutic targets.

Kaynak Göster

Bibtex @ { tbtkmedical577834, journal = {Turkish Journal of Medical Sciences}, issn = {1300-0144}, eissn = {1303-6165}, address = {}, publisher = {TÜBİTAK}, year = {2019}, volume = {49}, pages = {403 - 411}, doi = {}, title = {Determining the insulin secretion potential for certain specific G-protein coupled receptors in MIN6 pancreatic beta cells}, key = {cite}, author = {Gençoğlu, Hasan and Şahin, Kazim and Jones, Peter M.} }
APA Gençoğlu, H , Şahin, K , Jones, P . (2019). Determining the insulin secretion potential for certain specific G-protein coupled receptors in MIN6 pancreatic beta cells . Turkish Journal of Medical Sciences , 49 (1) , 403-411 . Retrieved from https://dergipark.org.tr/tr/pub/tbtkmedical/issue/45750/577834
MLA Gençoğlu, H , Şahin, K , Jones, P . "Determining the insulin secretion potential for certain specific G-protein coupled receptors in MIN6 pancreatic beta cells" . Turkish Journal of Medical Sciences 49 (2019 ): 403-411 <https://dergipark.org.tr/tr/pub/tbtkmedical/issue/45750/577834>
Chicago Gençoğlu, H , Şahin, K , Jones, P . "Determining the insulin secretion potential for certain specific G-protein coupled receptors in MIN6 pancreatic beta cells". Turkish Journal of Medical Sciences 49 (2019 ): 403-411
RIS TY - JOUR T1 - Determining the insulin secretion potential for certain specific G-protein coupled receptors in MIN6 pancreatic beta cells AU - Hasan Gençoğlu , Kazim Şahin , Peter M. Jones Y1 - 2019 PY - 2019 N1 - DO - T2 - Turkish Journal of Medical Sciences JF - Journal JO - JOR SP - 403 EP - 411 VL - 49 IS - 1 SN - 1300-0144-1303-6165 M3 - UR - Y2 - 2021 ER -
EndNote %0 Turkish Journal of Medical Sciences Determining the insulin secretion potential for certain specific G-protein coupled receptors in MIN6 pancreatic beta cells %A Hasan Gençoğlu , Kazim Şahin , Peter M. Jones %T Determining the insulin secretion potential for certain specific G-protein coupled receptors in MIN6 pancreatic beta cells %D 2019 %J Turkish Journal of Medical Sciences %P 1300-0144-1303-6165 %V 49 %N 1 %R %U
ISNAD Gençoğlu, Hasan , Şahin, Kazim , Jones, Peter M. . "Determining the insulin secretion potential for certain specific G-protein coupled receptors in MIN6 pancreatic beta cells". Turkish Journal of Medical Sciences 49 / 1 (Şubat 2019): 403-411 .
AMA Gençoğlu H , Şahin K , Jones P . Determining the insulin secretion potential for certain specific G-protein coupled receptors in MIN6 pancreatic beta cells. Turkish Journal of Medical Sciences. 2019; 49(1): 403-411.
Vancouver Gençoğlu H , Şahin K , Jones P . Determining the insulin secretion potential for certain specific G-protein coupled receptors in MIN6 pancreatic beta cells. Turkish Journal of Medical Sciences. 2019; 49(1): 403-411.
IEEE H. Gençoğlu , K. Şahin ve P. Jones , "Determining the insulin secretion potential for certain specific G-protein coupled receptors in MIN6 pancreatic beta cells", Turkish Journal of Medical Sciences, c. 49, sayı. 1, ss. 403-411, Şub. 2019

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