Asaad AZARNEZHAD, Mina TABRIZI, Firozeh JAVAN, Parvin MEHDIPOUR

Detection of CCND1, C-MYC, and FGFR1 amplification using modified SYBR Green qPCR and FISH in breast cancer

Öz Background/aim: The aims of this study were to detect CCND1, C-MYC, and FGFR1 amplification using qPCR, confirmation with FISH, and to further assess their clinicopathological relevance. Materials and methods: Thirty-five breast tumor samples were analyzed for amplification of the selected genes using modified SYBR Green qPCR. The accuracy of the qPCR was assessed by FISH as a gold-standard method. Results: CCND1, C-MYC, and FGFR1 amplifications were observed in 34.28%, 28.57%, and 17.14% of the 35 samples, respectively. qPCR results were significantly confirmed by FISH and qPCR and FISH showed excellent correlation (P = 0.000). CCND1 amplification with tumor stage (P = 0.044), positive metastatic status (P = 0.042), positive family history (P = 0.042), and C-MYC status (P = 0.005); C-MYC amplification with tumor size (P = 0.021), tumor grade (P = 0.018), tumor stage (P = 0.032), and FGFR1 status (P < 0.000); and FGFR1 amplification with tumor size (P = 0.041) and positive ER status (P = 0.042) were statistically associated. Conclusion: Our findings revealed that the applied qPCR approach could precisely quantify the relative gene copy number. More studies with a larger sample size are suggested to confirm the clinicopathological value of CCND1, C-MYC, and FGFR1 amplification.

Anahtar Kelimeler:

Breast cancer, fluorescent in situ hybridization, gene amplification, gene copy number, real-time polymerase chain reaction

Tam Metin

Kaynak Göster

Bibtex	`@araştırma makalesi { tbtkmedical576721, journal = {Turkish Journal of Medical Sciences}, issn = {1300-0144}, eissn = {1303-6165}, address = {}, publisher = {TÜBİTAK}, year = {2018}, volume = {48}, pages = {759 - 767}, doi = {}, title = {Detection of CCND1, C-MYC, and FGFR1 amplification using modified SYBR Green qPCR and FISH in breast cancer}, key = {cite}, author = {Azarnezhad, Asaad and Tabrızı, Mina and Javan, Firozeh and Mehdıpour, Parvin} }`
APA	Azarnezhad, A , Tabrızı, M , Javan, F , Mehdıpour, P . (2018). Detection of CCND1, C-MYC, and FGFR1 amplification using modified SYBR Green qPCR and FISH in breast cancer . Turkish Journal of Medical Sciences , 48 (4) , 759-767 . Retrieved from https://dergipark.org.tr/tr/pub/tbtkmedical/issue/45702/576721
MLA	Azarnezhad, A , Tabrızı, M , Javan, F , Mehdıpour, P . "Detection of CCND1, C-MYC, and FGFR1 amplification using modified SYBR Green qPCR and FISH in breast cancer" . Turkish Journal of Medical Sciences 48 (2018 ): 759-767 <https://dergipark.org.tr/tr/pub/tbtkmedical/issue/45702/576721>
Chicago	Azarnezhad, A , Tabrızı, M , Javan, F , Mehdıpour, P . "Detection of CCND1, C-MYC, and FGFR1 amplification using modified SYBR Green qPCR and FISH in breast cancer". Turkish Journal of Medical Sciences 48 (2018 ): 759-767
RIS	TY - JOUR T1 - Detection of CCND1, C-MYC, and FGFR1 amplification using modified SYBR Green qPCR and FISH in breast cancer AU - Asaad Azarnezhad , Mina Tabrızı , Firozeh Javan , Parvin Mehdıpour Y1 - 2018 PY - 2018 N1 - DO - T2 - Turkish Journal of Medical Sciences JF - Journal JO - JOR SP - 759 EP - 767 VL - 48 IS - 4 SN - 1300-0144-1303-6165 M3 - UR - Y2 - 2021 ER -
EndNote	%0 Turkish Journal of Medical Sciences Detection of CCND1, C-MYC, and FGFR1 amplification using modified SYBR Green qPCR and FISH in breast cancer %A Asaad Azarnezhad , Mina Tabrızı , Firozeh Javan , Parvin Mehdıpour %T Detection of CCND1, C-MYC, and FGFR1 amplification using modified SYBR Green qPCR and FISH in breast cancer %D 2018 %J Turkish Journal of Medical Sciences %P 1300-0144-1303-6165 %V 48 %N 4 %R %U
ISNAD	Azarnezhad, Asaad , Tabrızı, Mina , Javan, Firozeh , Mehdıpour, Parvin . "Detection of CCND1, C-MYC, and FGFR1 amplification using modified SYBR Green qPCR and FISH in breast cancer". Turkish Journal of Medical Sciences 48 / 4 (Ağustos 2018): 759-767 .
AMA	Azarnezhad A , Tabrızı M , Javan F , Mehdıpour P . Detection of CCND1, C-MYC, and FGFR1 amplification using modified SYBR Green qPCR and FISH in breast cancer. Turkish Journal of Medical Sciences. 2018; 48(4): 759-767.
Vancouver	Azarnezhad A , Tabrızı M , Javan F , Mehdıpour P . Detection of CCND1, C-MYC, and FGFR1 amplification using modified SYBR Green qPCR and FISH in breast cancer. Turkish Journal of Medical Sciences. 2018; 48(4): 759-767.
IEEE	A. Azarnezhad , M. Tabrızı , F. Javan ve P. Mehdıpour , "Detection of CCND1, C-MYC, and FGFR1 amplification using modified SYBR Green qPCR and FISH in breast cancer", Turkish Journal of Medical Sciences, c. 48, sayı. 4, ss. 759-767, Ağu. 2018