Göksu ELPEK, Gülay ÖZCENGİZ, Bülent İÇGEN

Colicinogeny, lipopolysaccharide and outer membrane Salmonella typhimurium isolates from Turkey

A total of 259 clinical isolates of non-repetitive non-typhi salmonellae (NTS) were previously examined for their antibiotic resistance patterns and plasmid contents. Multi-drug resistant strains comprised 19.3% (50/259) of the isolates and almost all were Salmonella typhimurium. In the present study, 35 of these multi-drug resistant S. typhimurium isolates were further characterized for colicinogeny, lipopolysaccharide (LPS) and outer membrane protein (OMP) profiles. Fourteen of the 35 (40%) isolates were found to be colicin producers. All isolates showed smooth-type LPS. In 26 of the (74.3%) isolates, OMPs of 30.6 and 34.6 kDa were observed whereas three (8.6%) of them were found to carry only a 30.6 kDa protein and six (17.1%) carried 27.2, 30.6 and 34.6 kDa proteins. There was no direct correlation between the plasmid contents, antibiotic resistance patterns, colicinogeny, LPS and OMP profiles of the isolates.

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1. Chang YH. Prevalence of Salmonella spp. in poultry broilers and shell eggs in Korea. J Food Protection 63: 655-658,20Q0.
2. Rajashekara G, Haverly E, Halvorsan DA, etal. M-ultidrug-resistant Salmonella typhimurium DT104 in poultry. J Food Protection 63: 155-161, 2000.
3. Tassios PT, Markogiannakis A,Vatopoulos AC, ef al. Molecular epidemiology of antibiotic resistance of 5. enteritidis during a 7-year period in Greece. J Clin Microbiol 35: 1316- 1321,1997.
4. Barker RM. Colicinogeny in Salmonella typhimurium. J Gen Microb 120: 21-6,1980.
5. Ward LR, De Sa JDH, Rowe B. A phage typing scheme for S. enteritidis. Epidemiol Infect 99: 291-304, 1987.
6. Millemann Y, Lesage MC, Chaslus-Dancla E, et al. Value of plasmid profiling, ribotyping and detection of IS200 for tracing avian isolates of Salmonella typhimurium and S. enteritidis. J Clin Microbiol 33: 173- 179, 1995.
7. Plate DJ, Brown DJ, Old DC, etal. Old and new techniques together resolve a problem of infection by S. tyhphimurium. Epidemiol Infect 99:137-142, 1987.
8. Davies RL. Outer membrane protein profiles of Yersinia ruckeri. Vet Microb 26: 125-140, 1991.
9. Blaser MJ, Hopkins JA, Berka RM, etal.Identification and characterization of Campylobacter jejuni outer membrane proteins. Infect Immun 42: 276-284,1983.
10. Threlfall EJ, Chart H. Special Article.Interrelationship between strains of Salmonella enteritidis. Epidemiol Infect 111: 1 -8, 1993.
11. Yıldırmak T, Yazgan A, Özcengiz G. Multiple drug resistance patterns and plasmid profiles of non-typhi salmonellae in Turkey. Epidemiol Infect 121:303-307, 1998.
12. Duguid J.P., Anderson E.S., Campbell I.: Fimbriae and adhesive properties in salmonellae. J Pathol Bacteriol 92: 107-138, 1966.
13. Hitchcock PJ,Brown TM.Morphological heterogeneity among Salmonella lipopolysaccharide chemotypes in silver-stained polyacrylamide gels. J Bacteriol 154:269-277, 1983.
14. Achtman M, Mercer A, Küsecek B, etal.Six widespread bacterial clones among Escherichia coll K1 isolates, infect Immun 39: 315-335, 1983.
15. Laemmli UK. Cleavegae of structural proteins during the assembly of the head of bacteriophage T4. Nature 227: 680-685/1970.
16. Tsai CM, Frasch CE. A sensitive silver stain for detecting LPS in polyacrylamide gels. Anal Biochem 119:115-119, 1982.
17. Martini A, Filetici E, Fantasia M.Epidemioiogicai markers of Salmonella typhimurium isolates in Rome. J Med Microbiol 37: 104-108, 1992.
18. Brockelsberg CA, Nolak LK, Brown J.Characterization of Salmonella dublin and Salmonella typhimurium (Copenhagen) isolates from cattle. Vet Res Commun 21: 409-20, 1997.
19. Threlfall EJ, Frost JA. The identification,typing and fingerprinting of Salmonella:laboratory aspects and epidemioiogicai applications. J Appl Bacteriol 68: 5-16,1990.
20. El-Khouly AE, Abou-Shleib H. Effect Of curing R-plasmids in Salmonella species on their serum sensitivity. Int j clin Pharm ThrTox 24: 433-437, 1986
21. Chart H, Threlfall EJ, Rowe B. Virulence of S. enteritidis phage type 4 is related to possession of a 38 M Da plasmid.FEMS Microbiol Lett 58: 299-304,1989a.
22. Chart H, Rowe B, Threifall EJ, et al.Conversion of S. enteritidis phage type 4 to phage type 7 involves loss of lipopolysaccharide with concomitant loss of virulence. FEMS Microbiol Lett 66: 37-40, 1989b.
23. Foster JW, Spector MP. How Salmonella survive against the odds.Ann Rev Microbiol 49: 145-74, 1995.
24. Gulig PA, Curtiss R. Plasmid-associated virulence of Salmonella typhimurium.Infect Immun 55: 2891-901, 1987.
25. Helmuth R, Stephan R, Bunge C, etal.Epidemiology of virulence-associated plasmids and outer membrane protein patterns within seven common Salmonella serovars. Infect Immun 48:175-182, 1985.
26. Poppe C, McFadden KA, Brouwer AM,et al. Characterization of S. enteritidis strains. Can J Res 57: 176-184, 1993.
27. Chart H, Rowe B. Antibodies to lipopolysaccharide and outer membrane proteins of 5. enteritidis FT 4 are not involved in protection from experimental infection. FEMS Microbiol Lett 84: 345-350, 1991.