Hojjatolah ZAMANI, Ali SALEHZADEH

Biofilm formation in uropathogenic Escherichia coli: association with adhesion factor genes

Background/aim: Biofilm formation is a major determinant factor in the development of urinary tract infections (UTIs) byuropathogenic Escherichia coli (UPEC). Several adhesion factors are involved in attachment of bacterial cells to the urinary tract andbiofilm development.Materials and methods: The possible relationship between different adhesion factor genes (AFGs) and biofilm formation among UPECisolates was investigated. Prevalence of different AFGs including fimA, fimH, papAH, papC, papEF, sfa-S, foc/G, and bmaE and theirassociation to biofilm formation were investigated. Phenotypic expression of type 1 and P fimbriae was also investigated.Results: Our results showed that 84% of UPEC were moderate to strong biofilm producers. Prevalence of the type 1 and P fimbriaeassociated genes (fimA, fimH, papEF, papC) as well as the F1C fimbriae gene (foc/G) did not show a difference among different biofilmproducing groups, while higher prevalence of papAH, bmaE (M fimbriae), and sfaS (S-fimbriae) was observed for the strains producingmoderate to strong biofilms. Phenotypic expression of type 1 fimbriae was associated with biofilm formation, while no association wasobserved for P fimbriae.Conclusion: Different AFGs (especially M and S fimbria) and their expression levels affect the biofilm formation ability of UPEC.

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Emo L, Kerenyi M, Nagy G. Virulence factors of uropathogenic Escherichia coli. Int J Antimicrob Agents 2003; 22: 29-33.
Ejrnaes K, Stegger M, Reisner A, Ferry S, Monsen T, Holm SE. Characteristics of Escherichia coli causing persistence or relapse of urinary tract infections: phylogenetic groups, virulence factors and biofilm formation. Virulence 2011; 2: 528-537.
Boll EJ, Struve C, Boisen N, Olesen B, Stahlhut SG, Krogfelt KA. Role of enteroaggregative Escherichia coli virulence factors in uropathogenesis. Infect Immune 2013; 81: 1164-1171.
Peirano G, Mulvey GL, Armstrong GD, Pitout JD. Virulence potential and adherence properties of Escherichia coli that produce CTX-M and NDM β-lactamases. J Med Microbial 2013; 62: 525-530.
Feraco D, Blaha M, Khan S, Green JM, Plotkin BJ. Host environmental signals and effects on biofilm formation. Microb Pathog 2016; 99: 253-263.
Khan AS, Kniep B, Oelschlaeger TA, Van Die I, Korhonen T, Hacker J. Receptor structure for F1 C fimbriae of uropathogenic Escherichia coli. Infect Immun 2000; 68: 3541-3547.
Oelschlaeger TA, Dobrindt U, Hacker J. Virulence factors of uropathogens. Curr Opin Urol 2012; 12: 33-38.
Das S, Dash HR. Microbial Biotechnology - A Laboratory Manual for Bacterial Systems. India: Springer; 2015.
Samet M, Ghaemi E, Hassan Nejadc M, Jamalid A. Prevalence of different virulence factors and biofilm production ability of urinary Escherichia coli isolates. Int J Biologic Med Res 2014; 5: 4546-4549.
Salo J, Sevander JJ, Tapiainen T, Ikäheimo I, Pokka T, Koskela M. Biofilm formation by Escherichia coli isolated from patients with urinary tract infections. Clin Nephrology 2009; 71: 501- 507.
Melican K, Sandoval RM, Kader A, Josefsson L, Tanner GA, Molitoris BA. Uropathogenic Escherichia coli P and Type 1 fimbriae act in synergy in a living host to facilitate renal colonization leading to nephron obstruction. PLoS Pathog 2011; 7: 1001298.
Schembri MA, Kjærgaard K, Klemm P. Global gene expression in Escherichia coli biofilms. Mol Microbiol 2003; 48: 253-267.
Soto SM, Smithson A, Martinez JA, Horcajada JP, Mensa J, Vila J. Biofilm formation in uropathogenic Escherichia coli strains: relationship with prostatitis, urovirulence factors and antimicrobial resistance. J Urol 2007; 177: 365-368.
Naves P, del Prado G, Huelves L, Gracia M, Ruiz V, Blanco J. Correlation between virulence factors and in vitro biofilm formation by Escherichia coli strains. Microb Pathog 2008; 45: 86-91.
Wang MC, Tseng CC, Wu AB, Lin WH, Teng CH, Yan JJ. Bacterial characteristics and glycemic control in diabetic patients with Escherichia coli urinary tract infection. J Microbiol Immunol Infect 2013; 46: 24-29.
Yun KW, Kim HY, Park HK, Kim W, Lim IS. Virulence factors of uropathogenic Escherichia coli of urinary tract infections and asymptomatic bacteriuria in children. J Microbiol Immun Infect 2014; 47: 455-461.
Kanamaru S, Kurazono H, Terai A, Monden K, Kumon H, Mizunoe Y. Increased biofilm formation in Escherichia coli isolated from acute prostatitis. Int J Antimicrob Agents 2006; 28: 21-25.
Reisner A, Krogfelt KA, Klein BM, Zechner EL, Molin S. In vitro biofilm formation of commensal and pathogenic Escherichia coli strains: impact of environmental and genetic factors. J Bacteriol 2006; 188: 3572-3581.
Fattahi S, Kafil HS, Nahai MR, Asgharzadeh M, Nori R, Aghazadeh M. Relationship of biofilm formation and different virulence genes in uropathogenic Escherichia coli isolates from Northwest Iran. GMS Hyg Infect Control 2015; 10: 1-7.
Floyd KA, Mitchell CA, Eberly A, Colling SJ, Zhang EW, DePas W. The UbiI (VisC) aerobic ubiquinone synthase is required for expression of type 1 pili, biofilm formation, and pathogenesis in uropathogenic Escherichia coli. J Bacteriol 2016; 198: 2662- 2672.
Zhao L, Gao S, Huan H, Xu X, Zhu X, Yang W. Comparison of virulence factors and expression of specific genes between uropathogenic Escherichia coli and avian pathogenic E. coli in a murine urinary tract infection model and a chicken challenge model. Microbiol 2009; 155: 1634-1644.
Johnson JR, Stell AL. Extended virulence genotypes of Escherichia coli strains from patients with urosepsis in relation to phylogeny and host compromise. J Infect Dis 2000; 181: 261- 272.