Mona Mohamed ZAKI, Hala ABDEL-AL, Mohamed AL-SAWI

Assessment of plasma amino acid profile in autism using cation-exchange chromatography with postcolumn derivatization by ninhydrin

Background/aim: Autism is a heterogeneous neurodevelopmental disorder. This study aimed to assess the clinical significance of amino acid profile assay in autism using cation-exchange chromatography with ninhydrin postcolumn derivatization. Materials and methods: This study included 42 autistic children and 26 apparently healthy children. All participants were subjected to the assay of plasma amino acids (essential, nonessential, and nonstandard) using cation-exchange chromatography with postcolumn derivatization by ninhydrin. Results: The levels of most of the essential amino acids were significantly lower in autistic children than controls. As regards nonessential amino acids, significantly lower levels for plasma cysteine, tyrosine, and serine and significantly higher levels for plasma glutamic acid were recorded in autistic children than controls. Finally, the autistic group demonstrated significantly lower levels of α-aminoadipic acid, carnosine, and β-alanine and significantly higher levels of hydroxyproline, phosphoserine, β-amino-isobutyric acid, and ammonia as compared to controls. Conclusion: The study revealed that autistic children exhibit distinct alterations in the plasma levels of some amino acids, which can in turn participate in the disease etiology and can be applied as a diagnostic tool for early detection of autism.

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1. Alanazi AS. The role of nutraceuticals in the management of autism. Saudi Pharm J 2013; 21: 233-243.
2. Lai MC, Lombardo MV, Baron-Cohen S. Autism. Lancet 2014; 383: 896-910.
3. Bauer AZ, Kriebel D. Prenatal and perinatal analgesic exposure and autism: an ecological link. Environ Health 2013; 12: 41.
4. Leblond CS, Heinrich J, Delorme R, Proepper C, Betancur C, Huguet G, Konyukh M, Chaste P, Ey E, Rastam M et al. Genetic and functional analyses of SHANK2 mutations suggest a multiple hit model of autism spectrum disorders. PLoS Genet 2012; 8: e1002521.
5. Tu WJ, Chen H, He J. Application of LC-MS/MS analysis of plasma amino acids profiles in children with autism. J Clin Biochem Nutr 2012; 51: 248-249.
6. Ghanizadeh A. Increased glutamate and homocysteine and decreased glutamine levels in autism: a review and strategies for future studies of amino acids in autism. Dis Markers 2013; 35: 281-286.
7. Lord C, Rutter M, Le Couteur A. Autism Diagnostic InterviewRevised: a revised version of a diagnostic interview for caregivers of individuals with possible pervasive developmental disorders. J Autism Dev Disord 1994; 24: 659-685.
8. Kim SH, Thurm A, Shumway S, Lord C. Multisite study of new autism diagnostic interview-revised (ADI-R) algorithms for toddlers and young preschoolers. J Autism Dev Disord 2013; 43: 1527-1538.
9. Csapó J, Albert C, Lóki K, Csapó-Kiss Z. Separation and determination of the amino acids by ion exchange column chromatography applying postcolumn derivatization. Acta Univ Sapientiae Alimentaria 2008; 1: 5-29.
10. Chang SC, Pauls DL, Lange C, Sasanfar R, Santangelo SL. Sexspecific association of a common variant of the XG gene with autism spectrum disorders. Am J Med Genet B Neuropsychiatr Genet 2013; 162B: 742-750.
11. Majewska MD, Hill M, Urbanowicz E, Rok-Bujko P, Bieńkowski P, Namysłowska I, Mierzejeweski P. Marked elevation of adrenal steroids, especially androgens, in saliva of prepubertal autistic children. Eur Child Adolesc Psychiatry 2014; 23: 485- 498.
12. Atladóttir HÓ, Henriksen TB, Schendel DE, Parner ET. Using maternally reported data to investigate the association between early childhood infection and autism spectrum disorder: the importance of data source. Paediatr Perinat Epidemiol 2012; 26: 373-385.
13. Farmer C, Thurm A, Grant P. Pharmacotherapy for the core symptoms in autistic disorder: current status of the research. Drugs 2013; 73: 303-314.
14. Xu XJ, Shou XJ, Li J, Jia MX, Zhang JS, Guo Y, Wei KY, Zhang XT, Han SP, Zhang R et al. Mothers of autistic children: lower plasma levels of oxytocin and arg-vasopressin and a higher level of testosterone. PLoS One 2013; 8: e74849.
15. Adams JB, Audhya T, McDonough-Means S, Rubin RA, Quig D, Geis E, Gehn E, Loresto M, Mitchell J, Atwood S et al. Nutritional and metabolic status of children with autism vs. neurotypical children, and the association with autism severity. Nutr Metab (Lond) 2011; 8: 34.
16. Tirouvanziam R, Obukhanych TV, Laval J, Aronov PA, Libove R, Banerjee AG, Parker KJ, OHara R, Herzenberg LA, Herzenberg LA et al. Distinct plasma profile of polar neutral amino acids, leucine, and glutamate in children with autism spectrum disorders. J Autism Dev Disord 2012; 42: 827-836.
17. Cubała-Kucharska M. The review of most frequently occurring medical disorders related to aetiology of autism and the methods of treatment. Acta Neurobiol Exp (Wars) 2010; 70: 141-146.
18. Naushad SM, Jain JN, Prasad CK, Naik U, Akella RR. Autistic children exhibit distinct amino acid profile. Indian J Biochem Biophys 2013; 50: 474-478.
19. Lakhan SE, Vieira KF. Nutritional therapies for mental disorders. Nutr J 2008; 7: 2.
20. Kałużna-Czaplińska J, Żurawicz E, Michalska M, Rynkowski J. A focus on homocysteine in autism. Acta Biochim Pol 2013; 60: 137-142.
21. Whiteley P, Haracopos D, Knivsberg AM, Reichelt KL, Parlar S, Jacobsen J, Seim A, Pedersen L, Schondel M, Shattock P. The ScanBrit randomised, controlled, single-blind study of a gluten- and casein-free dietary intervention for children with autism spectrum disorders. Nutr Neurosci 2010; 13: 87-100.
22. Xia W, Zhou Y, Sun C, Wang J, Wu L. A preliminary study on nutritional status and intake in Chinese children with autism. Eur J Pediatr 2010; 169: 1201-1206.
23. Waly MI, Hornig M, Trivedi M, Hodgson N, Kini R, Ohta A, Deth R. Prenatal and postnatal epigenetic programming: implications for GI, immune, and neuronal function in autism. Autism Research and Treatment 2012; 2012: 190930.
24. Main PEA, Angley MT, Thomas P, ODoherty CE, Fenech M. Folate and methionine metabolism in autism: a systematic review. Am J Clin Nutr 2010; 91: 1598-1620.
25. Santini MA, Balu DT, Puhl MD, Hill-Smith TE, Berg AR, Lucki I, Mikkelsen JD, Coyle JT. D-serine deficiency attenuates the behavioral and cellular effects induced by the hallucinogenic 5-HT (2A) receptor agonist. Behav Brain Res 2014; 259: 242- 246.
26. Shimmura C, Suda, S, Tsuchiya KJ, Hashimoto K, Ohno K, Matsuzaki H, Iwata K, Matsumoto K, Wakuda T, Kameno Y et al. Alteration of plasma glutamate and glutamine levels in children with high-functioning autism. PLoS One 2011; 6: e25340.