Analysis of promoter methylation of Dickkopf1 (DKK1) gene in breast cancer
Breast cancer is the most common malignancy in women worldwide. The Dickkopf1 (DKK1) gene product is a potent inhibitor of the Wnt pathway. DKK1 methylation status and its protein expression were examined in normal and cancer tissues of breast cancer patients. Materials and methods: We examined methylation changes in 83 tumor and adjacent normal tissues, and also in 9 normal breast tissues from unaffected women (no breast cancer history) as controls. DKK1 promoter methylation was assessed by methylation-specific polymerase chain reaction. Immunohistochemical staining was used to investigate DKK1 protein expression in the 9 controls and in 70 out of the 83 tumors as well as their adjacent normal tissues. Immunohistochemical results were categorized as positive or negative for DKK1. Results: DKK1 gene methylation was detected in 3 out of the 9 (33%) normal breast tissue samples of unaffected women. The DKK1 gene was hypermethylated in 58 out of the 83 (70%) tumor samples and 51 out of the 83 (61%) adjacent normal tissue samples. In immunohistochemistry, 33% of normal breast tissue from unaffected women, 31% of the tumors, and 17% of the adjacent normal tissues in the breast cancer patients were found to be DKK1-positive. DKK1 promoter methylation and protein expression were not associated with age, tumor size, lymph node status, histological grade, estrogen/progesterone receptor status, or HER2 positivity. Conclusion: Our results indicate that there is a concordant DKK1 methylation change between normal and cancerous breast tissue.
Analysis of promoter methylation of Dickkopf1 (DKK1) gene in breast cancer
Breast cancer is the most common malignancy in women worldwide. The Dickkopf1 (DKK1) gene product is a potent inhibitor of the Wnt pathway. DKK1 methylation status and its protein expression were examined in normal and cancer tissues of breast cancer patients. Materials and methods: We examined methylation changes in 83 tumor and adjacent normal tissues, and also in 9 normal breast tissues from unaffected women (no breast cancer history) as controls. DKK1 promoter methylation was assessed by methylation-specific polymerase chain reaction. Immunohistochemical staining was used to investigate DKK1 protein expression in the 9 controls and in 70 out of the 83 tumors as well as their adjacent normal tissues. Immunohistochemical results were categorized as positive or negative for DKK1. Results: DKK1 gene methylation was detected in 3 out of the 9 (33%) normal breast tissue samples of unaffected women. The DKK1 gene was hypermethylated in 58 out of the 83 (70%) tumor samples and 51 out of the 83 (61%) adjacent normal tissue samples. In immunohistochemistry, 33% of normal breast tissue from unaffected women, 31% of the tumors, and 17% of the adjacent normal tissues in the breast cancer patients were found to be DKK1-positive. DKK1 promoter methylation and protein expression were not associated with age, tumor size, lymph node status, histological grade, estrogen/progesterone receptor status, or HER2 positivity. Conclusion: Our results indicate that there is a concordant DKK1 methylation change between normal and cancerous breast tissue.