Dilek DEMİREL, Tuğba AYÇİÇEK, Seda GÜN, Sertaç HANCIOĞLU, Ünal BIÇAKCI, Ender ARITÜRK, Ferit BERNAY

Evaluation of the histopathological effects of salbutamol inhaler treatment in an experimentally induced rat model of pulmonary contusion

Background/aim: This study aimed to evaluate the effects of salbutamol inhaler treatment in an experimentally induced model of pulmonary contusion. Materials and methods: Thirty-two male Wistar albino rats were randomly divided into four groups: the control group (CG), sham group (SG), treatment group 1 (TG1), and treatment group 2 (TG2). Experimental contusion was established by targeting the right lung tissue. After 72 h, histopathological evaluation for the severity of edema, hemorrhage, and leukocyte infiltration was performed in both sides of the lungs. Results: Examination of right lung tissues revealed a significant difference in edema, hemorrhage, leukocyte infiltration, and total lung injury scores between the CG and SG. Both TG1 and TG2 had less edema, hemorrhage, and leukocyte infiltration and lower total lung injury scores compared with the SG. There was no significant difference in edema, hemorrhage, and total lung injury scores between the CG and TG1 or TG2. A significant difference in hemorrhage scores between the SG and TG1 and edema scores between the SG and TG2 was observed, with treatment groups having lower values. A significant difference in total lung injury score was also found between SG and TG1. Conclusion: Salbutamol inhaler therapy during pulmonary contusion may prevent complications by reducing edema, hemorrhage, leukocyte infiltration, and total lung injury score.

Keywords:

Experimental, pulmonary contusion, lung, salbutamol, inhalation, trauma,

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Peclet MH, Newman KD, Eichelberger MR, Gotschall CS, Garcia VF, Bowman LM. Thoracic trauma in children: an indicator of increased mortality. J Pediatr Surg 1990; 25: 961- 966.
Wu XJ, Xia ZY, Wang LL, Luo T, Zhan LY, Meng QT, Song XM. Effects of phencyclidine hydrochloride on pulmonary contusion from blunt chest trauma in rats. Injury 2012; 43: 232-236.
Bliss D, Silen M. Pediatric thoracic trauma. Crit Care Med 2002; 30: 409-415.
Akdemir HU, Güzel A, Katı C, Duran L, Alaçam H, Gacar A, Güvenç T, Murat N, Şişman B. The evaluation of different treatment protocols for trauma-induced lung injury in rats. J Thorac Dis 2014; 6: 66-73.
Ozdinc S, Oz G, Ozdemir C, Kilic I, Karakaya Z, Bal A, Koken T, Solak O. Melatonin: is it an effective antioxidant for pulmonary contusion? J Surg Res 2016; 204: 445-451.
Klassen TP, Rowe PC, Sutcliffe T, Ropp LJ, McDowell IW, Li MM. Randomized trial of salbutamol in acute bronchiolitis. J Pediatr 1991; 118: 807-811.
Eteraf-Oskouei T, Akbarzadeh-Atashkhosrow A, Maghsudi M, Najafi M. Effects of salbutamol on the inflammatory parameters and anjiogenesisi in the rat air pouch model of inflammation. Res Pharm Sci 2017; 12: 364-372.
McAuley DF, Frank JA, Fang X, Matthay MA. Clinically relevant concentrations of beta2-adrenergic agonists stimulate maximal cyclic adenosine monophosphate-dependent airspace fluid clearance and decrease pulmonary edema in experimental acid-induced lung injury. Crit Care Med 2004; 32: 1470-1476.
Perkins GD, Gates S, Park D, Gao F, Knox C, Holloway B, McAuley DF, Ryan J, Marzouk J, Cooke MW et al. The beta agonist lung injury trial prevention. A randomized controlled trial. Am J Respir Crit Care Med 2014; 189: 674-683.
Perkins GD, Nathani N, McAuley DF, Gao F, Thickett DR. In vitro and in vivo effects of salbutamol on neutrophil function in acute lung injury. Thorax 2007; 62: 36-42.
Roca O, Gomez-Olles S, Cruz MJ, Munoz X, Griffiths MJ, Masclans JR. Effects of salbutamol on exhaled breath condensate biomarkers in acute lung injury: prospective analysis. Crit Care 2008; 12: R72.
Akpinar ME, Tekke NS, Yigit O, Ercan F, Durna Y, Kiran D. Histological effects of inhaled corticosteroids and β2-agonists on laryngeal mucosa in allergic rat model. Otolaryngol Head Neck Surg 2013; 149: 457-465.
Raghavendran K, Davidson BA, Helinski JD, Marschke CJ, Manderscheid P, Woytash JA, Notter RH, Knight PR. A rat model for isolated bilateral lung contusion from blunt chest trauma. Anesth Analg 2005; 101: 1482-1489.
Yang Z, Deng Y, Su D, Tian J, Gao Y, He Z, Wang X. TLR4 as receptor for HMGB1-mediated acute lung injury after liver ischemia/reperfusion injury. Lab Invest 2013; 93: 792-800.
Holmes JF, Sokolove PE, Brant WE, Kuppermann N. A clinical decision rule for identifying children with thoracic injuries after blunt torso trauma. Ann Emerg Med 2002; 39: 492.
Cooper A, Barlow B, DiScala C, String D. Mortality and truncal injury: the pediatric perspective. J Pediatr Surg 1994; 29: 33-38.
Ganie FA, Lone H, Lone GN, Wani ML, Singh S, Dar AM, Wani N, Wani SN, Nazeer N. Lung contusion: a clinico-pathological entity with unpredictable clinical course. Bull Emerg Trauma 2013; 1: 7-16.
Peclet MH, Newman KD, Eichelberger MR, Gotschall CS, Garcia VF, Bowman LM. Thoracic trauma in children: an indicator of increased mortality. J Pediatr Surg 1990; 25: 961- 966.
Cohn SM, Dubose JJ. Pulmonary contusion: an update on recent advances in clinical management. World J Surg 2010; 34: 1959-1970.
Aksu B, Ayvaz S, Aksu F, Karaca T, Cemek M, Ayaz A, Demirtas S. Effects of sphingosylphosphorylcholine against oxidative stress and acute lung injury induced by pulmonary contusion in rats. J Pediatr Surg 2015; 50: 591-597.
Boybeyi O, Bakar B, Aslan MK, Atasoy P, Kisa U, Soyer T. Evaluation of dimethyl sulfoxide and dexamethasone on pulmonary contusion in experimental blunt thoracic trauma. Thorac Cardiovasc Surg 2014; 62: 710-715.
Ateş G, Yaman F, Bakar B, Kısa U, Atasoy P, Büyükkocak U. Evaluation of the systemic antiinflammatory effects of levosimendan in an experimental blunt thoracic trauma model. Ulus Travma Acil Cer 2017; 23: 368-376.
Cazzola M, Page CP, Rogliani P, Matera MG. β2-Agonist therapy in lung disease. Am J Respir Crit Care Med 2013; 187: 690-696.
Yilmaz S, Yildizdas D, Daglioglu K, Acikalin A, Acipayam C, Bayram I, Gumurdulu D, Tanyeli A. The effects of salbutamol in an experimental model with acute respiratory distress syndrome. J Acute Dis 2012; 1: 94-99.
O’Kane CM, McKeown SW, Perkins GHD, Bassford CR, Gao F, Thickett DR, McAuley DF. Salbutamol up-regulates matrix metalloproteinase-9 in the alveolar space in the acute respiratory distress syndrome. Crit Care Med 2009; 37: 2242- 2249.
Matthay MA, Brower RG, Carson S, Douglas IS, Eisner M, Hite D, Holets S, Kallet RH, Liu KD, MacIntyre N et al. Randomized, placebo-controlled clinical trial of an aerosolized β2-agonist for treatment of acute lung injury. Am J Respir Crit Care Med 2011; 184: 561-568.
Hellinger A, Konerding MA. Does lung contusion affect both the traumatized and the noninjured lung parenchyma? A morphological and morphometric study in the pig. J Trauma 1995; 39: 712-719.