We consider the Brezis-Nirenberg problem: $ -\triangle u=|u|^{p-1}u\pm\varepsilon u\mbox{ in }\Omega;, \mbox{ with } u=0 \mbox{ on }\partial\Omega,$ where $\Omega$ is a smooth bounded domain in $\mathbb{R}^n$, $n\geq4$, $p+1=2n/(n-2)$ is the critical Sobolev exponent, and $\varepsilon > 0$ is a positive parameter. The main result of this paper shows that if $n\geq4$ there are no sign-changing solutions $u_\varepsilon$ of $(P_{-\varepsilon})$ with two positive and one negative blow up points.